Restriction map for
pF1KB5588
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII BamHI
BbeI BbrPI BcgI BfrI BlpI
Bpu1102I BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BstAPI BstBI
BstEII BstPI Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 440 TCCGGA T^CCGGA
AcyI 759 GACGCC G[GA]^CG[CT]C
Aor51HI 509 AGCGCT AGC^GCT
ApaI 2159 GGGCCC GGGCC^C
AvrII 897 CCTAGG C^CTAGG
BanI 1889 GGTGCC G^G[CT][GA]CC
BbvCI 1875 CCTCAGC CCTCAGC(-5/-2)
BciVI 1324 GGATAC GTATCC(6/5)
BglII 12 AGATCT A^GATCT
BlnI 897 CCTAGG C^CTAGG
BsaAI 499 TACGTG [CT]AC^GT[GA]
BsaBI 633 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 759 GACGCC G[GA]^CG[CT]C
BsaI 1271 GAGACC GGTCTC(1/5)
BseAI 440 TCCGGA T^CCGGA
BsgI 1912 CTGCAC GTGCAG(16/14)
BsmI 1973 GAATGC GAATGC(1/-1)
BspEI 440 TCCGGA T^CCGGA
BsrBI 199 GAGCGG CCGCTC(-3/-3)
BsrDI 424 GCAATG GCAATG(2/0)
BsrFI 1103 GCCGGC [GA]^CCGG[CT]
BssSI 1600 CACGAG CACGAG(-5/-1)
Bst1107I 2076 GTATAC GTA^TAC
BstZ17I 2076 GTATAC GTA^TAC
Cfr10I 1103 GCCGGC [GA]^CCGG[CT]
Eco47III 509 AGCGCT AGC^GCT
Hin1I 759 GACGCC G[GA]^CG[CT]C
KspI 906 CCGCGG CCGC^GG
MamI 633 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 440 TCCGGA T^CCGGA
NaeI 1103 GCCGGC GCC^GGC
NcoI 1586 CCATGG C^CATGG
NgoMIV 1103 GCCGGC G^CCGGC
PflMI 572 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 550 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 805 CAGCTG CAG^CTG
SacII 906 CCGCGG CCGC^GG
SapI 918 GCTCTTC GCTCTTC(1/4)
SfiI 2004 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 989 CCCGGG CCC^GGG
SmlI 1047 CTTGAG C^T[CT][GA]AG
SphI 468 GCATGC GCATG^C
Tsp509I 157 AATT ^AATT
Van91I 572 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1284 TCTAGA T^CTAGA
XmaCI 989 CCCGGG C^CCGGG
XmaI 989 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 1639 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1825 GACAGGCAGTC
AspEI 1639 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1825 GACAGGCAGTC
AspHI 685 GAGCAC G[AT]GC[AT]^C
1666 GAGCAC
BbsI 1414 GAAGAC GAAGAC(2/6)
1654 GTCTTC
BclI 389 TGATCA T^GATCA
1278 TGATCA
BglI 26 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2005 GCCAGGGAGGC
BpuAI 1414 GAAGAC GAAGAC(2/6)
1654 GTCTTC
BsaWI 440 TCCGGA [AT]^CCGG[AT]
944 ACCGGA
BsiEI 414 CGGCCG CG[GA][CT]^CG
1499 CGGCCG
BsiHKAI 685 GAGCAC G[AT]GC[AT]^C
1666 GAGCAC
BspMI 1484 ACCTGC ACCTGC(4/8)
615 GCAGGT
EagI 414 CGGCCG C^GGCCG
1499 CGGCCG
Eam1105I 1639 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1825 GACAGGCAGTC
EclXI 414 CGGCCG C^GGCCG
1499 CGGCCG
Eco52I 414 CGGCCG C^GGCCG
1499 CGGCCG
FbaI 389 TGATCA T^GATCA
1278 TGATCA
HgaI 759 GACGC GACGC(5/10)
555 GCGTC
MseI 167 TTAA T^TAA
1526 TTAA
NspI 281 ACATGC [GA]CATG^[CT]
468 GCATGC
ScaI 173 AGTACT AGT^ACT
2132 AGTACT
SfcI 177 CTACAG C^T[GA][CT]AG
559 CTACAG
StuI 767 AGGCCT AGG^CCT
2153 AGGCCT
Tru9I 167 TTAA T^TAA
1526 TTAA
XcmI 1697 CCATCGTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1835 CCATGATCCTAGTGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AvaI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII