Restriction map for
pF1KB7015
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AlwI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BamHI
BciVI BfrI BlnI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI DraI
DraIII DrdI EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KpnI MfeI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI SalI SfuI
SgrAI SnaBI SpeI SspI SwaI
TfiI Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 2008 GTCTAC GT^[AC][GT]AC
AflIII 1283 ACGCGT A^C[GA][CT]GT
Aor51HI 740 AGCGCT AGC^GCT
BbrPI 868 CACGTG CAC^GTG
BcgI 1371 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2174 TGATCA T^GATCA
BfaI 961 CTAG C^TAG
BlpI 174 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
BsaBI 1890 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 291 GAATGC GAATGC(1/-1)
BsrDI 1862 GCAATG GCAATG(2/0)
BssSI 301 CACGAG CACGAG(-5/-1)
CelII 174 GCTGAGC GC^T[ACGT]AGC
CpoI 662 CGGACCG CG^G[AT]CCG
EarI 2107 CTCTTC CTCTTC(1/4)
Eco47III 740 AGCGCT AGC^GCT
FbaI 2174 TGATCA T^GATCA
Ksp632I 2107 CTCTTC CTCTTC(1/4)
MaeI 961 CTAG C^TAG
MamI 1890 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1283 ACGCGT A^CGCGT
NcoI 203 CCATGG C^CATGG
NspI 2193 GCATGC [GA]CATG^[CT]
PaeR7I 1369 CTCGAG C^TCGAG
PmaCI 868 CACGTG CAC^GTG
PmlI 868 CACGTG CAC^GTG
PshAI 1262 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 662 CGGACCG CG^G[AT]CCG
SapI 2106 GCTCTTC GCTCTTC(1/4)
SbfI 762 CCTGCAGG CCTGCA^GG
SphI 2193 GCATGC GCATG^C
Sse8387I 762 CCTGCAGG CCTGCA^GG
Tsp509I 1027 AATT ^AATT
XhoI 1369 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 2026 GACGTC GACGT^C
2262 GACGTC
Alw44I 1231 GTGCAC G^TGCAC
1924 GTGCAC
ApaLI 1231 GTGCAC G^TGCAC
1924 GTGCAC
AspI 2002 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2245 GACCGTGTC
AviII 1193 TGCGCA TGC^GCA
1313 TGCGCA
BbvCI 1128 CCTCAGC CCTCAGC(-5/-2)
421 GCTGAGG
BglII 1238 AGATCT A^GATCT
2057 AGATCT
BsaAI 868 CACGTG [CT]AC^GT[GA]
1654 TACGTG
BsgI 1632 CTGCAC GTGCAG(16/14)
2154 CTGCAC
BsiEI 500 CGGCCG CG[GA][CT]^CG
1959 CGGCCG
BsmAI 1360 GTCTC GTCTC(1/5)
1401 GTCTC
Bsp1407I 830 TGTACA T^GTACA
2168 TGTACA
BsrGI 830 TGTACA T^GTACA
2168 TGTACA
BstEII 1681 GGTAACC G^GT[ACGT]ACC
1872 GGTGACC
BstPI 1681 GGTAACC G^GT[ACGT]ACC
1872 GGTGACC
BstYI 1238 AGATCT [GA]^GATC[CT]
2057 AGATCT
EagI 500 CGGCCG C^GGCCG
1959 CGGCCG
EclXI 500 CGGCCG C^GGCCG
1959 CGGCCG
Eco52I 500 CGGCCG C^GGCCG
1959 CGGCCG
EcoNI 1431 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2204 CCTCCCAGAGG
EcoO65I 1681 GGTAACC G^GT[ACGT]ACC
1872 GGTGACC
FspI 1193 TGCGCA TGC^GCA
1313 TGCGCA
KspI 585 CCGCGG CCGC^GG
666 CCGCGG
MflI 1238 AGATCT [GA]^GATC[CT]
2057 AGATCT
PflFI 2002 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2245 GACCGTGTC
PstI 280 CTGCAG CTGCA^G
763 CTGCAG
PvuII 321 CAGCTG CAG^CTG
2227 CAGCTG
SacII 585 CCGCGG CCGC^GG
666 CCGCGG
ScaI 1817 AGTACT AGT^ACT
2306 AGTACT
SfiI 42 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
492 GGCCGTGCCGGCC
SspBI 830 TGTACA T^GTACA
2168 TGTACA
StuI 1212 AGGCCT AGG^CCT
1991 AGGCCT
Tth111I 2002 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2245 GACCGTGTC
XcmI 1205 CCAAGAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCAACACACCACTGG
XhoII 1238 AGATCT [GA]^GATC[CT]
2057 AGATCT
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XmaCI XmaI