Restriction map for
pF1KB7022
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AviII AvrII BamHI BbeI BbrPI
BclI BfrI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoT22I FbaI FspI KasI KspI
MfeI MluI MroI MunI NarI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 2509 GACGTC GACGT^C
Acc65I 1268 GGTACC G^GTACC
AccII 2342 CGCG CG^CG
AlwNI 1405 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1363 CCTGAGG CC^T[ACGT]AGG
Asp718I 1268 GGTACC G^GTACC
AspI 2485 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 703 GTCTTC GAAGAC(2/6)
BcgI 406 GCAGGCGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2614 GGATAC GTATCC(6/5)
BlpI 671 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 671 GCTCAGC GC^T[ACGT]AGC
BpuAI 703 GTCTTC GAAGAC(2/6)
BsaBI 558 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 411 CGTCTC CGTCTC(1/5)
Bsp1407I 1610 TGTACA T^GTACA
BsrBI 2758 GAGCGG CCGCTC(-3/-3)
BsrGI 1610 TGTACA T^GTACA
BssSI 1927 CACGAG CACGAG(-5/-1)
BstAPI 1404 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2342 CGCG CG^CG
BstXI 1156 CCAGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1363 CCTGAGG CC^T[ACGT]AGG
CelII 671 GCTCAGC GC^T[ACGT]AGC
DraIII 1390 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1540 CTCTTC CTCTTC(1/4)
Eco81I 1363 CCTGAGG CC^T[ACGT]AGG
EcoRV 2872 GATATC GAT^ATC
FseI 433 GGCCGGCC GGCCGG^CC
HaeII 280 AGCGCC [GA]GCGC^[CT]
HindIII 1048 AAGCTT A^AGCTT
HpaI 691 GTTAAC GTT^AAC
KpnI 1268 GGTACC GGTAC^C
Ksp632I 1540 CTCTTC CTCTTC(1/4)
MamI 558 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2342 CGCG CG^CG
NcoI 1962 CCATGG C^CATGG
NdeI 1218 CATATG CA^TATG
NheI 330 GCTAGC G^CTAGC
PaeR7I 2110 CTCGAG C^TCGAG
PflFI 2485 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1702 CAGCTG CAG^CTG
SalI 2089 GTCGAC G^TCGAC
ScaI 1991 AGTACT AGT^ACT
SexAI 1637 ACCAGGT A^CC[AT]GGT
SfiI 2670 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1610 TGTACA T^GTACA
Tsp509I 2317 AATT ^AATT
Tth111I 2485 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2110 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2089 GTCGAC GT^[AC][GT]AC
2491 GTCTAC
AhdI 2358 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2509 GACGTCTGGTC
AspEI 2358 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2509 GACGTCTGGTC
AvaI 2077 CCCGAG C^[CT]CG[GA]G
2110 CTCGAG
BbvCI 204 CCTCAGC CCTCAGC(-5/-2)
2035 GCTGAGG
BglI 1913 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2671 GCCTGCTGGGC
BglII 2540 AGATCT A^GATCT
2714 AGATCT
BsaI 263 GAGACC GGTCTC(1/5)
1423 GAGACC
BsoBI 2077 CCCGAG C^[CT]CG[GA]G
2110 CTCGAG
BspHI 1907 TCATGA T^CATGA
2408 TCATGA
BsrDI 131 GCAATG GCAATG(2/0)
2438 GCAATG
BsrFI 434 GCCGGC [GA]^CCGG[CT]
817 GCCGGC
BstEII 111 GGTGACC G^GT[ACGT]ACC
936 GGTGACC
BstPI 111 GGTGACC G^GT[ACGT]ACC
936 GGTGACC
BstYI 2540 AGATCT [GA]^GATC[CT]
2714 AGATCT
Cfr10I 434 GCCGGC [GA]^CCGG[CT]
817 GCCGGC
Eam1105I 2358 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2509 GACGTCTGGTC
EcoO65I 111 GGTGACC G^GT[ACGT]ACC
936 GGTGACC
MflI 2540 AGATCT [GA]^GATC[CT]
2714 AGATCT
MseI 692 TTAA T^TAA
1001 TTAA
NaeI 434 GCCGGC GCC^GGC
817 GCCGGC
NgoMIV 434 GCCGGC G^CCGGC
817 GCCGGC
NspI 813 ACATGC [GA]CATG^[CT]
1565 GCATGT
PleI 2203 GACTC GAGTC(4/5)
2416 GACTC
RcaI 1907 TCATGA T^CATGA
2408 TCATGA
SmlI 906 CTTGAG C^T[CT][GA]AG
2110 CTCGAG
Tru9I 692 TTAA T^TAA
1001 TTAA
XhoII 2540 AGATCT [GA]^GATC[CT]
2714 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI