Restriction map for
pF1KB3103
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BbeI BbrPI
BciVI BfaI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 505 GTAGAC GT^[AC][GT]AC
AflII 205 CTTAAG C^TTAAG
AspI 346 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 936 GCAGAAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 396 TGATCA T^GATCA
BfrI 205 CTTAAG C^TTAAG
BglII 1529 AGATCT A^GATCT
BlpI 1290 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1290 GCTGAGC GC^T[ACGT]AGC
BsaI 67 GAGACC GGTCTC(1/5)
BsiEI 498 CGACCG CG[GA][CT]^CG
BsmAI 67 GAGAC GTCTC(1/5)
BsmI 957 GAATGC GAATGC(1/-1)
Bsp1407I 1334 TGTACA T^GTACA
BspMI 339 GCAGGT ACCTGC(4/8)
BsrBI 1138 GAGCGG CCGCTC(-3/-3)
BsrFI 1607 GCCGGT [GA]^CCGG[CT]
BsrGI 1334 TGTACA T^GTACA
BstYI 1529 AGATCT [GA]^GATC[CT]
CelII 1290 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1607 GCCGGT [GA]^CCGG[CT]
CpoI 350 CGGTCCG CG^G[AT]CCG
DraIII 22 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1068 CCTCGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 396 TGATCA T^GATCA
HaeII 625 AGCGCC [GA]GCGC^[CT]
HincII 963 GTCAAC GT[CT]^[GA]AC
HindII 963 GTCAAC GT[CT]^[GA]AC
MflI 1529 AGATCT [GA]^GATC[CT]
MseI 206 TTAA T^TAA
NcoI 243 CCATGG C^CATGG
NspI 441 GCATGC [GA]CATG^[CT]
PaeR7I 1248 CTCGAG C^TCGAG
PflFI 346 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1122 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 350 CGGTCCG CG^G[AT]CCG
SapI 1617 GCTCTTC GCTCTTC(1/4)
SmaI 1425 CCCGGG CCC^GGG
SphI 441 GCATGC GCATG^C
SspBI 1334 TGTACA T^GTACA
Tru9I 206 TTAA T^TAA
Tth111I 346 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1122 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1160 CCAGGAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1248 CTCGAG C^TCGAG
XhoII 1529 AGATCT [GA]^GATC[CT]
XmaCI 1425 CCCGGG C^CCGGG
XmaI 1425 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 1248 CTCGAG C^[CT]CG[GA]G
1425 CCCGGG
AvaII 229 GGACC G^G[AT]CC
351 GGTCC
BalI 878 TGGCCA TGG^CCA
1130 TGGCCA
BanI 118 GGTGCC G^G[CT][GA]CC
143 GGCACC
BbsI 429 GAAGAC GAAGAC(2/6)
1005 GAAGAC
BbvCI 1222 CCTCAGC CCTCAGC(-5/-2)
171 GCTGAGG
BglI 1373 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1393 GCCACAGAGGC
BpmI 462 CTCCAG CTGGAG(16/14)
1171 CTGGAG
BpuAI 429 GAAGAC GAAGAC(2/6)
1005 GAAGAC
BseRI 1174 GAGGAG GAGGAG(10/8)
1234 GAGGAG
BsoBI 1248 CTCGAG C^[CT]CG[GA]G
1425 CCCGGG
BstXI 243 CCATGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1628 CCAACTATGTGG
DsaI 243 CCATGG C^C[GA][CT]GG
354 CCGTGG
EarI 1618 CTCTTC CTCTTC(1/4)
1153 GAAGAG
HgaI 46 GACGC GACGC(5/10)
961 GCGTC
HindIII 8 AAGCTT A^AGCTT
236 AAGCTT
Ksp632I 1618 CTCTTC CTCTTC(1/4)
1153 GAAGAG
MluNI 878 TGGCCA TGG^CCA
1130 TGGCCA
MscI 878 TGGCCA TGG^CCA
1130 TGGCCA
PvuII 293 CAGCTG CAG^CTG
769 CAGCTG
SfaNI 1267 GATGC GCATC(5/9)
195 GCATC
SfcI 407 CTGTAG C^T[GA][CT]AG
1296 CTACAG
SmlI 205 CTTAAG C^T[CT][GA]AG
1248 CTCGAG
TfiI 778 GAATC G^A[AT]TC
914 GATTC
TspRI 331 CACTG CA[GC]TG[ACGT][ACGT]^
465 CAGTG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI BanII BbvI
BcnI BmyI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI StyI
TaqI TseI Tsp45I Tsp509I TthHB8I