Restriction map for
pF1KB9466
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstEII BstPI BstUI
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FbaI FseI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI PvuII RsrII SacI SacII
SbfI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AclI 1569 AACGTT AA^CGTT
AflIII 1691 ACATGT A^C[GA][CT]GT
AocI 2026 CCTAAGG CC^T[ACGT]AGG
AseI 1885 ATTAAT AT^TAAT
AsnI 1885 ATTAAT AT^TAAT
AspHI 1269 GTGCTC G[AT]GC[AT]^C
AviII 216 TGCGCA TGC^GCA
BfaI 2089 CTAG C^TAG
BglI 515 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1194 GAGACC GGTCTC(1/5)
BsaWI 750 ACCGGA [AT]^CCGG[AT]
BsgI 858 CTGCAC GTGCAG(16/14)
BsiHKAI 1269 GTGCTC G[AT]GC[AT]^C
BsmBI 619 GAGACG CGTCTC(1/5)
BspDI 1393 ATCGAT AT^CGAT
BspHI 1676 TCATGA T^CATGA
BspLU11I 1691 ACATGT A^CATGT
BspMI 1856 ACCTGC ACCTGC(4/8)
Bst1107I 549 GTATAC GTA^TAC
BstAPI 427 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1403 TTCGAA TT^CGAA
BstXI 1101 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 549 GTATAC GTA^TAC
Bsu36I 2026 CCTAAGG CC^T[ACGT]AGG
CfoI 217 GCGC GCG^C
ClaI 1393 ATCGAT AT^CGAT
DraII 604 GGGTCCC [GA]G^G[ACGT]CC[CT]
Eco81I 2026 CCTAAGG CC^T[ACGT]AGG
EcoO109I 604 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 2173 ATGCAT ATGCA^T
FauI 685 GCGGG CCCGC(4/6)
FspI 216 TGCGCA TGC^GCA
HapII 751 CCGG C^CGG
HgaI 127 GCGTC GACGC(5/10)
HhaI 217 GCGC GCG^C
HinP1I 217 GCGC G^CGC
HincII 393 GTCGAC GT[CT]^[GA]AC
HindII 393 GTCGAC GT[CT]^[GA]AC
HpaII 751 CCGG C^CGG
MaeI 2089 CTAG C^TAG
MaeII 1570 ACGT A^CGT
MspA1I 2137 CAGCGG C[AC]G^C[GT]G
MspI 751 CCGG C^CGG
NsiI 2173 ATGCAT ATGCA^T
NspV 1403 TTCGAA TT^CGAA
PpuMI 604 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1885 ATTAAT AT^TAAT
Psp1406I 1569 AACGTT AA^CGTT
PstI 1786 CTGCAG CTGCA^G
RcaI 1676 TCATGA T^CATGA
SalI 393 GTCGAC G^TCGAC
SapI 2040 GAAGAGC GCTCTTC(1/4)
ScaI 1790 AGTACT AGT^ACT
SexAI 1743 ACCAGGT A^CC[AT]GGT
SfuI 1403 TTCGAA TT^CGAA
SphI 1423 GCATGC GCATG^C
SspI 2288 AATATT AAT^ATT
Tsp45I 1599 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccI 393 GTCGAC GT^[AC][GT]AC
549 GTATAC
AvaI 339 CTCGGG C^[CT]CG[GA]G
2248 CCCGAG
AvaII 605 GGTCC G^G[AT]CC
1243 GGACC
BanI 523 GGCACC G^G[CT][GA]CC
1097 GGTGCC
BglII 228 AGATCT A^GATCT
783 AGATCT
BmyI 891 GGGCAC G[AGT]GC[ACT]^C
1269 GTGCTC
BsoBI 339 CTCGGG C^[CT]CG[GA]G
2248 CCCGAG
Bsp1286I 891 GGGCAC G[AGT]GC[ACT]^C
1269 GTGCTC
EcoRI 1496 GAATTC G^AATTC
2242 GAATTC
EcoT14I 634 CCTTGG C^C[AT][AT]GG
1101 CCAAGG
SfcI 1511 CTACAG C^T[GA][CT]AG
1786 CTGCAG
StyI 634 CCTTGG C^C[AT][AT]GG
1101 CCAAGG
XcmI 1253 CCAAACTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1657 CCAGACTCAACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII