##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BmyI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AseI          112  ATTAAT               AT^TAAT
AsnI          112  ATTAAT               AT^TAAT
AvaI          209  CCCGGG               C^[CT]CG[GA]G
BglII         248  AGATCT               A^GATCT
BlpI           97  GCTCAGC              GC^T[ACGT]AGC
BpmI          172  CTGGAG               CTGGAG(16/14)
Bpu1102I       97  GCTCAGC              GC^T[ACGT]AGC
BsmFI         206  GTCCC                GGGAC(10/14)
BsoBI         209  CCCGGG               C^[CT]CG[GA]G
BspMI         127  ACCTGC               ACCTGC(4/8)
BstYI         248  AGATCT               [GA]^GATC[CT]
Cac8I          93  GCTTGC               GC[ACGT]^[ACGT]GC
CelII          97  GCTCAGC              GC^T[ACGT]AGC
DpnII         249  GATC                 ^GATC
HapII         210  CCGG                 C^CGG
HindIII       322  AAGCTT               A^AGCTT
HpaII         210  CCGG                 C^CGG
HphI           19  TCACC                GGTGA(8/7)
MboI          249  GATC                 ^GATC
MboII         224  TCTTC                GAAGA(8/7)
MflI          248  AGATCT               [GA]^GATC[CT]
MseI          113  TTAA                 T^TAA
MspA1I         71  CAGCTG               C[AC]G^C[GT]G
MspI          210  CCGG                 C^CGG
NdeII         249  GATC                 ^GATC
NlaIV         275  GGGTCC               GG[ACGT]^[ACGT]CC
PshBI         112  ATTAAT               AT^TAAT
PstI           41  CTGCAG               CTGCA^G
PvuII          71  CAGCTG               CAG^CTG
Sau3AI        249  GATC                 ^GATC
SmaI          209  CCCGGG               CCC^GGG
Tru9I         113  TTAA                 T^TAA
TspRI         202  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         248  AGATCT               [GA]^GATC[CT]
XmaCI         209  CCCGGG               C^CCGGG
XmaI          209  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwNI          68  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              166  CAGAGGCTG           
AvaII         188  GGACC                G^G[AT]CC
              276  GGTCC               
BcnI          209  CCCGG                CC^[GC]GG
              210  CCGGG               
Cfr13I        188  GGACC                G^G[ACGT]CC
              276  GGTCC               
DdeI           98  CTCAG                C^T[ACGT]AG
              161  CTAAG               
MslI           47  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              266  CACAGAAGTG          
MwoI           37  GCCTCTGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               93  GCTTGCTCAGC         
NciI          209  CCCGG                CC^[GC]GG
              210  CCGGG               
NlaIII         12  CATG                 CATG^
               30  CATG                
Sau96I        188  GGACC                G^G[ACGT]CC
              276  GGTCC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsrI        BssKI       
BstNI       EcoRII      Fnu4HI      ItaI        MnlI        
MvaI        ScrFI       SfcI        TseI