##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      CpoI        DdeI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MflI        MluI        
MluNI       MroI        MscI        MspI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AluI          246  AGCT                 AG^CT
AlwI           45  GATCC                GGATC(4/5)
BbvI          247  GCTGC                GCAGC(8/12)
BmyI          384  GGGCAC               G[AGT]GC[ACT]^C
BsmAI         339  GAGAC                GTCTC(1/5)
Bsp1286I      384  GGGCAC               G[AGT]GC[ACT]^C
BspDI         133  ATCGAT               AT^CGAT
BsrI           61  CCAGT                ACTGG(1/-1)
BssKI         252  CCTGG                ^CC[ACGT]GG
BstNI         252  CCTGG                CC^[AT]GG
ClaI          133  ATCGAT               AT^CGAT
EcoRII        252  CCTGG                ^CC[AT]GG
Fnu4HI        247  GCTGC                GC^[ACGT]GC
HphI          256  GGTGA                GGTGA(8/7)
ItaI          247  GCTGC                GC^[ACGT]GC
MfeI          171  CAATTG               C^AATTG
MnlI           37  CCTC                 CCTC(7/6)
MslI          208  CATATCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        245  CAGCTG               C[AC]G^C[GT]G
MunI          171  CAATTG               C^AATTG
MvaI          252  CCTGG                CC^[AT]GG
PvuII         245  CAGCTG               CAG^CTG
ScrFI         252  CCTGG                CC^[ACGT]GG
TaqI          134  TCGA                 T^CGA
TseI          247  GCTGC                G^C[AT]GC
TthHB8I       134  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcsI          187  GAATTT               [GA]^AATT[CT]
              374  AAATTT              
ApoI          187  GAATTT               [GA]^AATT[CT]
              374  AAATTT              
BsaJI         251  CCCTGG               C^C[ACGT][ACGT]GG
              252  CCTGGG              
DpnII          45  GATC                 ^GATC
              136  GATC                
DraI          199  TTTAAA               TTT^AAA
              297  TTTAAA              
MboI           45  GATC                 ^GATC
              136  GATC                
MboII         236  GAAGA                GAAGA(8/7)
              283  GAAGA               
NdeII          45  GATC                 ^GATC
              136  GATC                
NlaIII          7  CATG                 CATG^
              153  CATG                
Sau3AI         45  GATC                 ^GATC
              136  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

MseI        SfaNI       Tru9I       Tsp509I