Restriction map for
pF1KB6883
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HindIII HpaI HphI KasI
KpnI KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NarI NciI NcoI
NdeI NlaIV NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 10 CGCG CG^CG
AfaI 417 GTAC GT^AC
AlwI 351 GATCC GGATC(4/5)
AspHI 99 GAGCAC G[AT]GC[AT]^C
AvaII 393 GGACC G^G[AT]CC
BbsI 139 GAAGAC GAAGAC(2/6)
BfaI 214 CTAG C^TAG
BglI 106 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 24 GCTGAGC GC^T[ACGT]AGC
BmyI 99 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
BpuAI 139 GAAGAC GAAGAC(2/6)
BsiEI 37 CGGCCG CG[GA][CT]^CG
BsiHKAI 99 GAGCAC G[AT]GC[AT]^C
BsiYI 14 CCGTGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 14 CCGTGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 333 GTCTC GTCTC(1/5)
Bsp1286I 99 GAGCAC G[AGT]GC[ACT]^C
BspHI 410 TCATGA T^CATGA
BsrFI 20 GCCGGC [GA]^CCGG[CT]
BstUI 10 CGCG CG^CG
CelII 24 GCTGAGC GC^T[ACGT]AGC
CfoI 11 GCGC GCG^C
Cfr10I 20 GCCGGC [GA]^CCGG[CT]
Cfr13I 393 GGACC G^G[ACGT]CC
DdeI 25 CTGAG C^T[ACGT]AG
DrdI 285 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 37 CGGCCG [CT]^GGCC[GA]
EagI 37 CGGCCG C^GGCCG
EarI 478 GAAGAG CTCTTC(1/4)
EclXI 37 CGGCCG C^GGCCG
Eco52I 37 CGGCCG C^GGCCG
EcoRI 184 GAATTC G^AATTC
HapII 21 CCGG C^CGG
HgaI 249 GACGC GACGC(5/10)
HhaI 11 GCGC GCG^C
HinP1I 11 GCGC G^CGC
HincII 316 GTCAAC GT[CT]^[GA]AC
HindII 316 GTCAAC GT[CT]^[GA]AC
HinfI 129 GAATC G^A[ACGT]TC
HpaII 21 CCGG C^CGG
Ksp632I 478 GAAGAG CTCTTC(1/4)
MaeI 214 CTAG C^TAG
MaeII 320 ACGT A^CGT
MspI 21 CCGG C^CGG
MvnI 10 CGCG CG^CG
NaeI 20 GCCGGC GCC^GGC
NgoMIV 20 GCCGGC G^CCGGC
NheI 213 GCTAGC G^CTAGC
NotI 36 GCGGCCGC GC^GGCCGC
NspI 217 GCATGT [GA]CATG^[CT]
PstI 509 CTGCAG CTGCA^G
RcaI 410 TCATGA T^CATGA
RsaI 417 GTAC GT^AC
Sau96I 393 GGACC G^G[ACGT]CC
SmlI 94 CTCAAG C^T[CT][GA]AG
StuI 302 AGGCCT AGG^CCT
TfiI 129 GAATC G^A[AT]TC
TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 54 CCACGACAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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BglII 275 AGATCT A^GATCT
398 AGATCT
BpmI 388 CTCCAG CTGGAG(16/14)
124 CTGGAG
BsaJI 122 CCCTGG C^C[ACGT][ACGT]GG
473 CCGAGG
BstF5I 51 CATCC GGATG(2/0)
119 CATCC
Cac8I 20 GCCGGC GC[ACGT]^[ACGT]GC
213 GCTAGC
DraI 174 TTTAAA TTT^AAA
456 TTTAAA
FokI 51 CATCC GGATG(9/13)
119 CATCC
HaeIII 38 GGCC GG^CC
303 GGCC
SfcI 426 CTACAG C^T[GA][CT]AG
509 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BssKI BstNI BstYI DpnII EcoRII
Fnu4HI ItaI MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI Tru9I
TseI Tsp509I XhoII