Restriction map for
pF1KB3086
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TthHB8I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 21 CGCG CG^CG
AclI 1669 AACGTT AA^CGTT
AcyI 94 GACGCC G[GA]^CG[CT]C
Alw44I 1735 GTGCAC G^TGCAC
AocI 199 CCTAAGG CC^T[ACGT]AGG
ApaLI 1735 GTGCAC G^TGCAC
AspI 493 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 8 GGACC G^G[AT]CC
AviII 14 TGCGCA TGC^GCA
BalI 1703 TGGCCA TGG^CCA
BanI 153 GGTGCC G^G[CT][GA]CC
BfaI 677 CTAG C^TAG
BlpI 45 GCTCAGC GC^T[ACGT]AGC
BpmI 885 CTGGAG CTGGAG(16/14)
Bpu1102I 45 GCTCAGC GC^T[ACGT]AGC
BsaHI 94 GACGCC G[GA]^CG[CT]C
BsaI 964 GGTCTC GGTCTC(1/5)
BseRI 596 GAGGAG GAGGAG(10/8)
Bsp1407I 929 TGTACA T^GTACA
BspMI 859 GCAGGT ACCTGC(4/8)
BsrBI 114 CCGCTC CCGCTC(-3/-3)
BsrDI 833 GCAATG GCAATG(2/0)
BsrGI 929 TGTACA T^GTACA
BssSI 1282 CTCGTG CACGAG(-5/-1)
BstAPI 17 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 21 CGCG CG^CG
BstXI 1706 CCATCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 199 CCTAAGG CC^T[ACGT]AGG
CelII 45 GCTCAGC GC^T[ACGT]AGC
DraIII 18 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 199 CCTAAGG CC^T[ACGT]AGG
EcoNI 1461 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 572 CCTTGG C^C[AT][AT]GG
FspI 14 TGCGCA TGC^GCA
HgaI 94 GACGC GACGC(5/10)
Hin1I 94 GACGCC G[GA]^CG[CT]C
MaeI 677 CTAG C^TAG
MluNI 1703 TGGCCA TGG^CCA
MscI 1703 TGGCCA TGG^CCA
MvnI 21 CGCG CG^CG
PflFI 493 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 770 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1669 AACGTT AA^CGTT
PvuII 271 CAGCTG CAG^CTG
SacI 295 GAGCTC GAGCT^C
ScaI 881 AGTACT AGT^ACT
SmaI 60 CCCGGG CCC^GGG
SspBI 929 TGTACA T^GTACA
StyI 572 CCTTGG C^C[AT][AT]GG
Tth111I 493 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 770 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 60 CCCGGG C^CCGGG
XmaI 60 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 439 GAATTT [GA]^AATT[CT]
627 GAATTT
AlwI 175 GGATC GGATC(4/5)
680 GGATC
ApaI 204 GGGCCC GGGCC^C
1359 GGGCCC
ApoI 439 GAATTT [GA]^AATT[CT]
627 GAATTT
AspHI 295 GAGCTC G[AT]GC[AT]^C
1735 GTGCAC
AvaI 60 CCCGGG C^[CT]CG[GA]G
1663 CTCGGG
BclI 716 TGATCA T^GATCA
729 TGATCA
BglII 1072 AGATCT A^GATCT
1092 AGATCT
BsiHKAI 295 GAGCTC G[AT]GC[AT]^C
1735 GTGCAC
BsoBI 60 CCCGGG C^[CT]CG[GA]G
1663 CTCGGG
FauI 78 CCCGC CCCGC(4/6)
1680 CCCGC
FbaI 716 TGATCA T^GATCA
729 TGATCA
MspA1I 271 CAGCTG C[AC]G^C[GT]G
1681 CCGCTG
NspI 211 ACATGC [GA]CATG^[CT]
926 GCATGT
PleI 143 GACTC GAGTC(4/5)
411 GACTC
PstI 133 CTGCAG CTGCA^G
1227 CTGCAG
SfaNI 39 GATGC GCATC(5/9)
1600 GATGC
StuI 338 AGGCCT AGG^CCT
555 AGGCCT
TspRI 665 CACTG CA[GC]TG[ACGT][ACGT]^
1370 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I XhoII XmnI