Restriction map for
pF1KB3125
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbvCI BclI BglI BlnI BlpI
Bpu1102I BsaI BsmBI BsmI BspDI
BspHI BsrFI BssHII BssSI BstBI
BstEII BstPI CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1265 GTATAC GT^[AC][GT]AC
AccII 42 CGCG CG^CG
AccIII 1697 TCCGGA T^CCGGA
AflII 688 CTTAAG C^TTAAG
AocI 1563 CCTCAGG CC^T[ACGT]AGG
AvaI 484 CCCGAG C^[CT]CG[GA]G
BanI 754 GGCGCC G^G[CT][GA]CC
BbeI 754 GGCGCC GGCGC^C
BbrPI 1372 CACGTG CAC^GTG
BbsI 6 GTCTTC GAAGAC(2/6)
BciVI 1297 GGATAC GTATCC(6/5)
BfrI 688 CTTAAG C^TTAAG
BpuAI 6 GTCTTC GAAGAC(2/6)
BsaAI 1372 CACGTG [CT]AC^GT[GA]
BsaBI 1701 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1697 TCCGGA [AT]^CCGG[AT]
BseAI 1697 TCCGGA T^CCGGA
BsiEI 246 CGACCG CG[GA][CT]^CG
BsiWI 83 CGTACG C^GTACG
BsoBI 484 CCCGAG C^[CT]CG[GA]G
Bsp1407I 770 TGTACA T^GTACA
BspEI 1697 TCCGGA T^CCGGA
BsrBI 47 GAGCGG CCGCTC(-3/-3)
BsrDI 938 GCAATG GCAATG(2/0)
BsrGI 770 TGTACA T^GTACA
Bst1107I 1265 GTATAC GTA^TAC
BstAPI 333 GCAAACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 42 CGCG CG^CG
BstZ17I 1265 GTATAC GTA^TAC
Bsu36I 1563 CCTCAGG CC^T[ACGT]AGG
DraIII 1606 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1563 CCTCAGG CC^T[ACGT]AGG
EcoNI 1746 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1378 GATATC GAT^ATC
HaeII 754 GGCGCC [GA]GCGC^[CT]
HgaI 4 GCGTC GACGC(5/10)
HincII 508 GTTGAC GT[CT]^[GA]AC
HindII 508 GTTGAC GT[CT]^[GA]AC
KasI 754 GGCGCC G^GCGCC
MamI 1701 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1697 TCCGGA T^CCGGA
MspA1I 962 CAGCTG C[AC]G^C[GT]G
MvnI 42 CGCG CG^CG
NarI 754 GGCGCC GG^CGCC
NcoI 1655 CCATGG C^CATGG
NdeI 644 CATATG CA^TATG
PflMI 435 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1372 CACGTG CAC^GTG
PmlI 1372 CACGTG CAC^GTG
PstI 513 CTGCAG CTGCA^G
PvuII 962 CAGCTG CAG^CTG
SacI 469 GAGCTC GAGCT^C
ScaI 797 AGTACT AGT^ACT
SexAI 91 ACCTGGT A^CC[AT]GGT
SfcI 513 CTGCAG C^T[GA][CT]AG
SfoI 754 GGCGCC GGC^GCC
SspBI 770 TGTACA T^GTACA
Van91I 435 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 980 AAATTC [GA]^AATT[CT]
1495 GAATTT
AcyI 3 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
AflIII 635 ACATGT A^C[GA][CT]GT
767 ACATGT
ApoI 980 AAATTC [GA]^AATT[CT]
1495 GAATTT
BanII 130 GGGCTC G[GA]GC[CT]^C
469 GAGCTC
BcgI 246 CGACCGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
28 GCAACCTCCTCG
BfaI 299 CTAG C^TAG
598 CTAG
BglII 137 AGATCT A^GATCT
303 AGATCT
BpmI 493 CTGGAG CTGGAG(16/14)
602 CTGGAG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
BsgI 896 CTGCAC GTGCAG(16/14)
1292 GTGCAG
BspLU11I 635 ACATGT A^CATGT
767 ACATGT
BspMI 68 ACCTGC ACCTGC(4/8)
224 ACCTGC
DsaI 108 CCACGG C^C[GA][CT]GG
1655 CCATGG
EaeI 623 CGGCCA [CT]^GGCC[GA]
1365 TGGCCG
FauI 257 CCCGC CCCGC(4/6)
43 GCGGG
Hin1I 3 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
MaeI 299 CTAG C^TAG
598 CTAG
MaeIII 869 GTCAC ^GT[ACGT]AC
1195 GTCAC
MslI 405 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1111 CATGTCGATG
SmlI 133 CTCAAG C^T[CT][GA]AG
688 CTTAAG
Tsp45I 869 GTCAC ^GT[GC]AC
1195 GTCAC
XcmI 404 CCACCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
567 CCAGCTCTCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII