Restriction map for
pF1KB3466
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AocI 256 CCTGAGG CC^T[ACGT]AGG
AspI 796 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 512 GGATCC G^GATCC
BanII 1112 GGGCTC G[GA]GC[CT]^C
BcgI 11 CGATCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1469 CTAG C^TAG
BglI 1195 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 588 AGATCT A^GATCT
BsmFI 1223 GGGAC GGGAC(10/14)
BsmI 326 GCATTC GAATGC(1/-1)
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BstAPI 1417 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 256 CCTGAGG CC^T[ACGT]AGG
DraII 1568 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 1189 GAAGAG CTCTTC(1/4)
Eco81I 256 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1568 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 296 AGCGCC [GA]GCGC^[CT]
HgaI 1176 GCGTC GACGC(5/10)
Ksp632I 1189 GAAGAG CTCTTC(1/4)
MaeI 1469 CTAG C^TAG
MaeII 1521 ACGT A^CGT
MslI 897 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 32 CCATGG C^CATGG
PflFI 796 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 92 ACCAGGT A^CC[AT]GGT
SfiI 1194 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 542 GCATGC GCATG^C
Tth111I 796 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 390 CCAGTCTAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 760 AAATTT [GA]^AATT[CT]
1717 GAATTT
AlwNI 295 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1418 CAGAGCCTG
ApoI 760 AAATTT [GA]^AATT[CT]
1717 GAATTT
Asp700I 274 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
609 GAACTACTTC
AvaI 926 CCCGGG C^[CT]CG[GA]G
1220 CCCGGG
BlpI 1081 GCTCAGC GC^T[ACGT]AGC
1381 GCTGAGC
BmyI 1112 GGGCTC G[AGT]GC[ACT]^C
1142 GTGCCC
Bpu1102I 1081 GCTCAGC GC^T[ACGT]AGC
1381 GCTGAGC
BsaI 358 GAGACC GGTCTC(1/5)
670 GGTCTC
BsgI 904 GTGCAG GTGCAG(16/14)
1552 GTGCAG
BsoBI 926 CCCGGG C^[CT]CG[GA]G
1220 CCCGGG
Bsp1286I 1112 GGGCTC G[AGT]GC[ACT]^C
1142 GTGCCC
CelII 1081 GCTCAGC GC^T[ACGT]AGC
1381 GCTGAGC
DsaI 32 CCATGG C^C[GA][CT]GG
537 CCGTGG
FauI 351 CCCGC CCCGC(4/6)
459 CCCGC
HphI 99 GGTGA GGTGA(8/7)
192 GGTGA
MaeIII 25 GTGAC ^GT[ACGT]AC
193 GTGAC
MseI 233 TTAA T^TAA
827 TTAA
PstI 139 CTGCAG CTGCA^G
1210 CTGCAG
PvuII 1246 CAGCTG CAG^CTG
1288 CAGCTG
SfcI 139 CTGCAG C^T[GA][CT]AG
1210 CTGCAG
SmaI 926 CCCGGG CCC^GGG
1220 CCCGGG
StuI 1202 AGGCCT AGG^CCT
1214 AGGCCT
TaqI 1061 TCGA T^CGA
1278 TCGA
TfiI 279 GATTC G^A[AT]TC
1280 GAATC
Tru9I 233 TTAA T^TAA
827 TTAA
Tsp45I 25 GTGAC ^GT[GC]AC
193 GTGAC
TthHB8I 1061 TCGA T^CGA
1278 TCGA
XmaCI 926 CCCGGG C^CCGGG
1220 CCCGGG
XmaI 926 CCCGGG C^CCGGG
1220 CCCGGG
XmnI 274 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
609 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BbsI BbvI BcnI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TseI Tsp509I TspRI XhoII