Restriction map for
pF1KB3486
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspHI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DraII
DraIII EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 991 GACGTC GACGT^C
AccII 191 CGCG CG^CG
AccIII 887 TCCGGA T^CCGGA
AcyI 991 GACGTC G[GA]^CG[CT]C
AhdI 1045 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 21 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1045 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 152 TGGCCA TGG^CCA
BcgI 850 GCAGCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 896 CTAG C^TAG
BsaAI 1075 TACGTG [CT]AC^GT[GA]
BsaHI 991 GACGTC G[GA]^CG[CT]C
BsaWI 887 TCCGGA [AT]^CCGG[AT]
BseAI 887 TCCGGA T^CCGGA
BsmAI 616 GAGAC GTCTC(1/5)
BsmFI 76 GGGAC GGGAC(10/14)
BspDI 1015 ATCGAT AT^CGAT
BspEI 887 TCCGGA T^CCGGA
BsrBI 1066 CCGCTC CCGCTC(-3/-3)
BsrDI 338 CATTGC GCAATG(2/0)
BstAPI 681 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 191 CGCG CG^CG
BstXI 578 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1015 ATCGAT AT^CGAT
DrdI 394 GACATCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 297 CCACGG C^C[GA][CT]GG
Eam1105I 1045 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 369 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 334 GACGC GACGC(5/10)
Hin1I 991 GACGTC G[GA]^CG[CT]C
HindIII 737 AAGCTT A^AGCTT
MaeI 896 CTAG C^TAG
MluNI 152 TGGCCA TGG^CCA
MroI 887 TCCGGA T^CCGGA
MscI 152 TGGCCA TGG^CCA
MvnI 191 CGCG CG^CG
NaeI 349 GCCGGC GCC^GGC
NgoMIV 349 GCCGGC G^CCGGC
PflMI 552 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 172 CTGCAG CTGCA^G
SfcI 172 CTGCAG C^T[GA][CT]AG
SmaI 776 CCCGGG CCC^GGG
SmlI 718 CTCAAG C^T[CT][GA]AG
TspRI 51 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 552 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 359 CCATGAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 776 CCCGGG C^CCGGG
XmaI 776 CCCGGG C^CCGGG
XmnI 21 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 776 CCCGGG C^[CT]CG[GA]G
955 CCCGAG
BanII 781 GAGCCC G[GA]GC[CT]^C
952 GAGCCC
BglI 187 GCCTCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1049 GCCAGTCGGGC
BlpI 196 GCTAAGC GC^T[ACGT]AGC
1068 GCTCAGC
Bpu1102I 196 GCTAAGC GC^T[ACGT]AGC
1068 GCTCAGC
BseRI 541 GAGGAG GAGGAG(10/8)
571 GAGGAG
BsgI 828 CTGCAC GTGCAG(16/14)
688 GTGCAG
BsoBI 776 CCCGGG C^[CT]CG[GA]G
955 CCCGAG
BspMI 170 ACCTGC ACCTGC(4/8)
630 GCAGGT
BsrFI 349 GCCGGC [GA]^CCGG[CT]
677 ACCGGC
CelII 196 GCTAAGC GC^T[ACGT]AGC
1068 GCTCAGC
Cfr10I 349 GCCGGC [GA]^CCGG[CT]
677 ACCGGC
EaeI 152 TGGCCA [CT]^GGCC[GA]
1047 CGGCCA
EcoT14I 578 CCAAGG C^C[AT][AT]GG
792 CCTTGG
MaeII 992 ACGT A^CGT
1076 ACGT
MspA1I 43 CAGCTG C[AC]G^C[GT]G
664 CAGCTG
PvuII 43 CAGCTG CAG^CTG
664 CAGCTG
StyI 578 CCAAGG C^C[AT][AT]GG
792 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SphI TaqI TseI Tsp45I
TthHB8I XhoII