Restriction map for
pF1KB8290
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AvrII BamHI BanII
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CpoI
DraII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoRI EcoT22I FauI FbaI FseI
HaeII HindIII HpaI KasI Ksp632I
KspI MamI MluI MroI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 1561 GGTACC G^GTACC
AciI 447 GCGG CCGC(-3/-1)
AcsI 34 GAATTT [GA]^AATT[CT]
AcyI 383 GGCGTC G[GA]^CG[CT]C
AflIII 66 ACATGT A^C[GA][CT]GT
AlwNI 887 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 34 GAATTT [GA]^AATT[CT]
Asp718I 1561 GGTACC G^GTACC
AspI 373 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 762 CTCGGG C^[CT]CG[GA]G
AvaII 1484 GGACC G^G[AT]CC
AviII 239 TGCGCA TGC^GCA
BalI 1281 TGGCCA TGG^CCA
BbvCI 1467 GCTGAGG CCTCAGC(-5/-2)
BcnI 1033 CCCGG CC^[GC]GG
BsaAI 547 TACGTG [CT]AC^GT[GA]
BsaHI 383 GGCGTC G[GA]^CG[CT]C
BsmFI 1425 GGGAC GGGAC(10/14)
BsoBI 762 CTCGGG C^[CT]CG[GA]G
BspDI 1415 ATCGAT AT^CGAT
BspLU11I 66 ACATGT A^CATGT
BspMI 98 ACCTGC ACCTGC(4/8)
BsrBI 445 GAGCGG CCGCTC(-3/-3)
BsrDI 466 CATTGC GCAATG(2/0)
BsrFI 1614 ACCGGC [GA]^CCGG[CT]
BstEII 616 GGTTACC G^GT[ACGT]ACC
BstPI 616 GGTTACC G^GT[ACGT]ACC
BstXI 696 CCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 390 GGATCT [GA]^GATC[CT]
CfoI 240 GCGC GCG^C
Cfr10I 1614 ACCGGC [GA]^CCGG[CT]
Cfr13I 1484 GGACC G^G[ACGT]CC
ClaI 1415 ATCGAT AT^CGAT
DraI 124 TTTAAA TTT^AAA
EcoNI 360 CCTGCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 616 GGTTACC G^GT[ACGT]ACC
EcoRV 1163 GATATC GAT^ATC
EcoT14I 425 CCTTGG C^C[AT][AT]GG
FspI 239 TGCGCA TGC^GCA
HgaI 384 GCGTC GACGC(5/10)
HhaI 240 GCGC GCG^C
Hin1I 383 GGCGTC G[GA]^CG[CT]C
HinP1I 240 GCGC G^CGC
HincII 709 GTCAAC GT[CT]^[GA]AC
HindII 709 GTCAAC GT[CT]^[GA]AC
KpnI 1561 GGTACC GGTAC^C
MaeII 548 ACGT A^CGT
MflI 390 GGATCT [GA]^GATC[CT]
MluNI 1281 TGGCCA TGG^CCA
MscI 1281 TGGCCA TGG^CCA
NciI 1033 CCCGG CC^[GC]GG
NheI 1570 GCTAGC G^CTAGC
NspI 66 ACATGT [GA]CATG^[CT]
PflFI 373 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 886 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 100 CTGCAG CTGCA^G
Sau96I 1484 GGACC G^G[ACGT]CC
SbfI 99 CCTGCAGG CCTGCA^GG
ScaI 119 AGTACT AGT^ACT
Sse8387I 99 CCTGCAGG CCTGCA^GG
StyI 425 CCTTGG C^C[AT][AT]GG
Tth111I 373 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 886 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1412 CCAATCGATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 390 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1183 CCTCAGG CC^T[ACGT]AGG
1394 CCTCAGG
AspHI 277 GTGCTC G[AT]GC[AT]^C
1008 GAGCAC
BanI 1302 GGCACC G^G[CT][GA]CC
1561 GGTACC
BbvI 803 GCAGC GCAGC(8/12)
889 GCTGC
BfaI 394 CTAG C^TAG
1571 CTAG
BmyI 277 GTGCTC G[AGT]GC[ACT]^C
1008 GAGCAC
BsaI 1439 GAGACC GGTCTC(1/5)
782 GGTCTC
BsgI 550 GTGCAG GTGCAG(16/14)
1322 GTGCAG
BsiHKAI 277 GTGCTC G[AT]GC[AT]^C
1008 GAGCAC
Bsp1286I 277 GTGCTC G[AGT]GC[ACT]^C
1008 GAGCAC
BspHI 452 TCATGA T^CATGA
750 TCATGA
Bsu36I 1183 CCTCAGG CC^T[ACGT]AGG
1394 CCTCAGG
DraIII 482 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1052 CACACAGTG
EaeI 1281 TGGCCA [CT]^GGCC[GA]
1616 CGGCCA
Eco81I 1183 CCTCAGG CC^T[ACGT]AGG
1394 CCTCAGG
HaeIII 1282 GGCC GG^CC
1617 GGCC
HapII 1034 CCGG C^CGG
1615 CCGG
HpaII 1034 CCGG C^CGG
1615 CCGG
MaeI 394 CTAG C^TAG
1571 CTAG
MfeI 77 CAATTG C^AATTG
719 CAATTG
MseI 125 TTAA T^TAA
518 TTAA
MslI 67 CATGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1315 CATCCCAGTG
MspA1I 887 CAGCTG C[AC]G^C[GT]G
1465 CAGCTG
MspI 1034 CCGG C^CGG
1615 CCGG
MunI 77 CAATTG C^AATTG
719 CAATTG
PleI 647 GAGTC GAGTC(4/5)
707 GAGTC
PvuII 887 CAGCTG CAG^CTG
1465 CAGCTG
RcaI 452 TCATGA T^CATGA
750 TCATGA
SfcI 100 CTGCAG C^T[GA][CT]AG
854 CTACAG
SmlI 622 CTTGAG C^T[CT][GA]AG
1406 CTCAAG
Tru9I 125 TTAA T^TAA
518 TTAA
TseI 803 GCAGC G^C[AT]GC
889 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AlwI BbsI BciVI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI TaqI TfiI Tsp45I
Tsp509I TspRI TthHB8I