Restriction map for
pF1KB4113
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiHKAI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrGI
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RsrII
SacI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 259 TCCGGA T^CCGGA
AgeI 1331 ACCGGT A^CCGGT
Aor51HI 35 AGCGCT AGC^GCT
AseI 207 ATTAAT AT^TAAT
AsnI 207 ATTAAT AT^TAAT
BalI 857 TGGCCA TGG^CCA
BciVI 256 GTATCC GTATCC(6/5)
BclI 1304 TGATCA T^GATCA
BglII 429 AGATCT A^GATCT
BmyI 1231 GTGCCC G[AGT]GC[ACT]^C
BseAI 259 TCCGGA T^CCGGA
BsgI 239 CTGCAC GTGCAG(16/14)
BsiWI 924 CGTACG C^GTACG
Bsp1286I 1231 GTGCCC G[AGT]GC[ACT]^C
BspEI 259 TCCGGA T^CCGGA
BssHII 276 GCGCGC G^CGCGC
BstEII 1248 GGTGACC G^GT[ACGT]ACC
BstPI 1248 GGTGACC G^GT[ACGT]ACC
BstXI 950 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 429 AGATCT [GA]^GATC[CT]
DrdI 537 GACAGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 35 AGCGCT AGC^GCT
EcoO65I 1248 GGTGACC G^GT[ACGT]ACC
FbaI 1304 TGATCA T^GATCA
HaeII 35 AGCGCT [GA]GCGC^[CT]
KspI 77 CCGCGG CCGC^GG
MflI 429 AGATCT [GA]^GATC[CT]
MluNI 857 TGGCCA TGG^CCA
MroI 259 TCCGGA T^CCGGA
MscI 857 TGGCCA TGG^CCA
MseI 208 TTAA T^TAA
MslI 951 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 611 GCATGT [GA]CATG^[CT]
PflMI 363 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1331 ACCGGT A^CCGGT
PshAI 1341 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 207 ATTAAT AT^TAAT
PvuII 554 CAGCTG CAG^CTG
SacII 77 CCGCGG CCGC^GG
SalI 1315 GTCGAC G^TCGAC
SexAI 1043 ACCAGGT A^CC[AT]GGT
SspI 831 AATATT AAT^ATT
StuI 467 AGGCCT AGG^CCT
Tru9I 208 TTAA T^TAA
Van91I 363 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 138 TCTAGA T^CTAGA
XhoII 429 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1315 GTCGAC GT^[AC][GT]AC
1528 GTAGAC
AlwI 520 GATCC GGATC(4/5)
1058 GGATC
BbsI 820 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BpmI 164 CTGGAG CTGGAG(16/14)
1408 CTGGAG
BpuAI 820 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BsaWI 259 TCCGGA [AT]^CCGG[AT]
1331 ACCGGT
BspHI 560 TCATGA T^CATGA
983 TCATGA
BsrDI 309 CATTGC GCAATG(2/0)
1308 CATTGC
BsrFI 9 ACCGGC [GA]^CCGG[CT]
1331 ACCGGT
Cfr10I 9 ACCGGC [GA]^CCGG[CT]
1331 ACCGGT
EarI 990 CTCTTC CTCTTC(1/4)
541 GAAGAG
FauI 903 CCCGC CCCGC(4/6)
1285 CCCGC
Ksp632I 990 CTCTTC CTCTTC(1/4)
541 GAAGAG
NlaIV 1105 GGGACC GG[ACGT]^[ACGT]CC
1322 GGCTCC
RcaI 560 TCATGA T^CATGA
983 TCATGA
XcmI 98 CCACAGGAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1397 CCAAGAAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I AvaII BbvI BfaI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI