Restriction map for
pF1KB4277
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FauI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 310 GAATTT [GA]^AATT[CT]
AfaI 1010 GTAC GT^AC
AflII 922 CTTAAG C^TTAAG
AhdI 1022 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 734 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 386 GGGCCC GGGCC^C
ApoI 310 GAATTT [GA]^AATT[CT]
Asp700I 174 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1022 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1271 GGTCC G^G[AT]CC
BamHI 1254 GGATCC G^GATCC
BanII 386 GGGCCC G[GA]GC[CT]^C
BciVI 765 GGATAC GTATCC(6/5)
BfrI 922 CTTAAG C^TTAAG
BsgI 250 GTGCAG GTGCAG(16/14)
BsmBI 514 CGTCTC CGTCTC(1/5)
BstEII 974 GGTCACC G^GT[ACGT]ACC
BstPI 974 GGTCACC G^GT[ACGT]ACC
DraII 385 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 519 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eam1105I 1022 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 385 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 974 GGTCACC G^GT[ACGT]ACC
EcoRV 1128 GATATC GAT^ATC
EcoT14I 1159 CCTTGG C^C[AT][AT]GG
MspA1I 878 CAGCTG C[AC]G^C[GT]G
PflMI 111 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 878 CAGCTG CAG^CTG
RsaI 1010 GTAC GT^AC
SmlI 922 CTTAAG C^T[CT][GA]AG
SspI 957 AATATT AAT^ATT
StyI 1159 CCTTGG C^C[AT][AT]GG
TfiI 1146 GAATC G^A[AT]TC
Van91I 111 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 174 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbvI 16 GCAGC GCAGC(8/12)
252 GCAGC
BclI 47 TGATCA T^GATCA
744 TGATCA
BmyI 386 GGGCCC G[AGT]GC[ACT]^C
571 GGGCAC
BpmI 118 CTGGAG CTGGAG(16/14)
491 CTGGAG
BsiYI 111 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
631 CCAAAGGAGGG
BslI 111 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
631 CCAAAGGAGGG
BsmFI 205 GGGAC GGGAC(10/14)
614 GGGAC
BsmI 185 GCATTC GAATGC(1/-1)
930 GCATTC
Bsp1286I 386 GGGCCC G[AGT]GC[ACT]^C
571 GGGCAC
FbaI 47 TGATCA T^GATCA
744 TGATCA
Fnu4HI 16 GCAGC GC^[ACGT]GC
252 GCAGC
ItaI 16 GCAGC GC^[ACGT]GC
252 GCAGC
MseI 923 TTAA T^TAA
1039 TTAA
MslI 946 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1194 CATGCAAGTG
MwoI 811 GCTCACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1197 GCAAGTGTGGC
PshAI 508 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1265 GACAAGGGTC
TaqI 23 TCGA T^CGA
192 TCGA
Tru9I 923 TTAA T^TAA
1039 TTAA
TseI 16 GCAGC G^C[AT]GC
252 GCAGC
TthHB8I 23 TCGA T^CGA
192 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI BbsI BpuAI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
FokI HaeIII HgaI HinfI HphI
MaeIII MboI MboII MflI MnlI
MvaI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI Tsp45I
Tsp509I TspRI XhoII