Restriction map for
pF1KB4461
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BsrBI
BssHII Bst1107I BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1314 CGCG CG^CG
AflIII 371 ACATGT A^C[GA][CT]GT
AlwI 1064 GGATC GGATC(4/5)
Aor51HI 625 AGCGCT AGC^GCT
AvaII 507 GGACC G^G[AT]CC
BcnI 1334 CCGGG CC^[GC]GG
BsgI 1619 CTGCAC GTGCAG(16/14)
Bsp1407I 944 TGTACA T^GTACA
BspLU11I 371 ACATGT A^CATGT
BspMI 36 GCAGGT ACCTGC(4/8)
BsrFI 89 ACCGGC [GA]^CCGG[CT]
BsrGI 944 TGTACA T^GTACA
BssSI 351 CACGAG CACGAG(-5/-1)
BstAPI 1521 GCATCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 515 TTCGAA TT^CGAA
BstEII 1341 GGTCACC G^GT[ACGT]ACC
BstPI 1341 GGTCACC G^GT[ACGT]ACC
BstUI 1314 CGCG CG^CG
Cfr10I 89 ACCGGC [GA]^CCGG[CT]
EarI 523 CTCTTC CTCTTC(1/4)
Eco47III 625 AGCGCT AGC^GCT
EcoO65I 1341 GGTCACC G^GT[ACGT]ACC
HaeII 625 AGCGCT [GA]GCGC^[CT]
HgaI 1307 GCGTC GACGC(5/10)
HindIII 1058 AAGCTT A^AGCTT
Ksp632I 523 CTCTTC CTCTTC(1/4)
MspA1I 454 CAGCTG C[AC]G^C[GT]G
MvnI 1314 CGCG CG^CG
NciI 1334 CCGGG CC^[GC]GG
NcoI 359 CCATGG C^CATGG
NspI 371 ACATGT [GA]CATG^[CT]
NspV 515 TTCGAA TT^CGAA
PvuII 454 CAGCTG CAG^CTG
ScaI 396 AGTACT AGT^ACT
SfuI 515 TTCGAA TT^CGAA
SmlI 445 CTCAAG C^T[CT][GA]AG
SspBI 944 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BciVI 1503 GGATAC GTATCC(6/5)
1322 GTATCC
BglI 341 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1294 GCCACCCTGGC
BmyI 348 GGGCAC G[AGT]GC[ACT]^C
1077 GGGCAC
BpmI 294 CTCCAG CTGGAG(16/14)
700 CTGGAG
BsaBI 681 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
843 GATCATGATC
BsmI 50 GAATGC GAATGC(1/-1)
1645 GAATGC
Bsp1286I 348 GGGCAC G[AGT]GC[ACT]^C
1077 GGGCAC
BspHI 254 TCATGA T^CATGA
845 TCATGA
DraII 459 GGGGCCT [GA]G^G[ACGT]CC[CT]
695 AGGGCCT
EaeI 339 TGGCCG [CT]^GGCC[GA]
1316 CGGCCA
EcoO109I 459 GGGGCCT [GA]G^G[ACGT]CC[CT]
695 AGGGCCT
EcoT14I 359 CCATGG C^C[AT][AT]GG
1705 CCTTGG
MaeII 722 ACGT A^CGT
1713 ACGT
MamI 681 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
843 GATCATGATC
PstI 392 CTGCAG CTGCA^G
1686 CTGCAG
RcaI 254 TCATGA T^CATGA
845 TCATGA
SfcI 392 CTGCAG C^T[GA][CT]AG
1686 CTGCAG
StuI 318 AGGCCT AGG^CCT
966 AGGCCT
StyI 359 CCATGG C^C[AT][AT]GG
1705 CCTTGG
TaqI 516 TCGA T^CGA
1524 TCGA
TthHB8I 516 TCGA T^CGA
1524 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BclI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I TspRI
XcmI