Restriction map for
pF1KB5019
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeIII MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 1509 GGATC GGATC(4/5)
Aor51HI 18 AGCGCT AGC^GCT
AspHI 951 GAGCAC G[AT]GC[AT]^C
AvaI 27 CCCGGG C^[CT]CG[GA]G
AvrII 352 CCTAGG C^CTAGG
BbsI 745 GAAGAC GAAGAC(2/6)
BlnI 352 CCTAGG C^CTAGG
BlpI 1266 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1266 GCTCAGC GC^T[ACGT]AGC
BpuAI 745 GAAGAC GAAGAC(2/6)
BsaBI 1072 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1018 GAGGAG GAGGAG(10/8)
BsiHKAI 951 GAGCAC G[AT]GC[AT]^C
BsmFI 232 GTCCC GGGAC(10/14)
BsoBI 27 CCCGGG C^[CT]CG[GA]G
BspMI 334 GCAGGT ACCTGC(4/8)
BsrDI 1118 GCAATG GCAATG(2/0)
BstYI 1509 GGATCT [GA]^GATC[CT]
CelII 1266 GCTCAGC GC^T[ACGT]AGC
DpnII 1510 GATC ^GATC
DsaI 50 CCGCGG C^C[GA][CT]GG
EaeI 1407 TGGCCG [CT]^GGCC[GA]
EarI 1083 GAAGAG CTCTTC(1/4)
Eco47III 18 AGCGCT AGC^GCT
EcoRV 296 GATATC GAT^ATC
HapII 28 CCGG C^CGG
HpaII 28 CCGG C^CGG
Ksp632I 1083 GAAGAG CTCTTC(1/4)
KspI 50 CCGCGG CCGC^GG
MaeII 77 ACGT A^CGT
MamI 1072 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1510 GATC ^GATC
MflI 1509 GGATCT [GA]^GATC[CT]
MslI 556 CATACGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 28 CCGG C^CGG
NdeI 1331 CATATG CA^TATG
NdeII 1510 GATC ^GATC
NheI 904 GCTAGC G^CTAGC
PflMI 827 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 50 CCGCGG CCGC^GG
Sau3AI 1510 GATC ^GATC
SbfI 1056 CCTGCAGG CCTGCA^GG
SmaI 27 CCCGGG CCC^GGG
SmlI 531 CTTGAG C^T[CT][GA]AG
Sse8387I 1056 CCTGCAGG CCTGCA^GG
Van91I 827 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1509 GGATCT [GA]^GATC[CT]
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 51 CGCG CG^CG
1028 CGCG
AvaII 349 GGACC G^G[AT]CC
397 GGACC
BanII 126 GGGCTC G[GA]GC[CT]^C
1237 GAGCCC
BcnI 27 CCCGG CC^[GC]GG
28 CCGGG
BsmAI 183 GAGAC GTCTC(1/5)
570 GTCTC
BsrI 833 ACTGG ACTGG(1/-1)
683 CCAGT
BstF5I 873 GGATG GGATG(2/0)
1302 GGATG
BstUI 51 CGCG CG^CG
1028 CGCG
EcoT14I 352 CCTAGG C^C[AT][AT]GG
1210 CCTTGG
FauI 56 CCCGC CCCGC(4/6)
133 GCGGG
FokI 873 GGATG GGATG(9/13)
1302 GGATG
HaeII 18 AGCGCT [GA]GCGC^[CT]
36 GGCGCT
HphI 325 GGTGA GGTGA(8/7)
576 GGTGA
MspA1I 50 CCGCGG C[AC]G^C[GT]G
65 CCGCTG
MvnI 51 CGCG CG^CG
1028 CGCG
NciI 27 CCCGG CC^[GC]GG
28 CCGGG
PstI 206 CTGCAG CTGCA^G
1057 CTGCAG
StyI 352 CCTAGG C^C[AT][AT]GG
1210 CCTTGG
TfiI 484 GATTC G^A[AT]TC
966 GATTC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MaeI MboII MnlI MseI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I