Restriction map for
pF1KE0971
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanII BbrPI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MroI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 79 GGCGCC G[GA]^CG[CT]C
AflII 189 CTTAAG C^TTAAG
AseI 748 ATTAAT AT^TAAT
AsnI 748 ATTAAT AT^TAAT
AspHI 785 GAGCAC G[AT]GC[AT]^C
BalI 352 TGGCCA TGG^CCA
BanI 79 GGCGCC G^G[CT][GA]CC
BbeI 79 GGCGCC GGCGC^C
BcnI 1560 CCCGG CC^[GC]GG
BfrI 189 CTTAAG C^TTAAG
BsaAI 788 CACGTA [CT]AC^GT[GA]
BsaBI 1583 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsiHKAI 785 GAGCAC G[AT]GC[AT]^C
BsmI 1213 GCATTC GAATGC(1/-1)
BsrDI 254 GCAATG GCAATG(2/0)
DraI 1593 TTTAAA TTT^AAA
DrdI 115 GACTGGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 75 CCGCGG C^C[GA][CT]GG
FauI 104 CCCGC CCCGC(4/6)
HapII 1561 CCGG C^CGG
HgaI 122 GCGTC GACGC(5/10)
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HpaII 1561 CCGG C^CGG
KasI 79 GGCGCC G^GCGCC
KspI 75 CCGCGG CCGC^GG
MamI 1583 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 352 TGGCCA TGG^CCA
MscI 352 TGGCCA TGG^CCA
MslI 964 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1561 CCGG C^CGG
NarI 79 GGCGCC GG^CGCC
NciI 1560 CCCGG CC^[GC]GG
NdeI 767 CATATG CA^TATG
NspI 290 ACATGC [GA]CATG^[CT]
PshBI 748 ATTAAT AT^TAAT
SacII 75 CCGCGG CCGC^GG
SapI 1241 GCTCTTC GCTCTTC(1/4)
SfoI 79 GGCGCC GGC^GCC
TfiI 1030 GAATC G^A[AT]TC
XcmI 1053 CCATGAAAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 76 CGCG CG^CG
121 CGCG
AfaI 1433 GTAC GT^AC
1516 GTAC
BamHI 1039 GGATCC G^GATCC
1316 GGATCC
BclI 1221 TGATCA T^GATCA
1403 TGATCA
BmyI 785 GAGCAC G[AGT]GC[ACT]^C
818 GGGCAC
BseRI 416 CTCCTC GAGGAG(10/8)
1097 CTCCTC
Bsp1286I 785 GAGCAC G[AGT]GC[ACT]^C
818 GGGCAC
BspHI 432 TCATGA T^CATGA
1406 TCATGA
BstUI 76 CGCG CG^CG
121 CGCG
DraII 1084 AGGTCCC [GA]G^G[ACGT]CC[CT]
1447 AGGACCT
EaeI 131 TGGCCG [CT]^GGCC[GA]
352 TGGCCA
EarI 1242 CTCTTC CTCTTC(1/4)
892 GAAGAG
EcoO109I 1084 AGGTCCC [GA]G^G[ACGT]CC[CT]
1447 AGGACCT
EcoT14I 387 CCTTGG C^C[AT][AT]GG
1488 CCAAGG
FbaI 1221 TGATCA T^GATCA
1403 TGATCA
HaeII 22 AGCGCC [GA]GCGC^[CT]
79 GGCGCC
Ksp632I 1242 CTCTTC CTCTTC(1/4)
892 GAAGAG
MaeII 789 ACGT A^CGT
1287 ACGT
MspA1I 75 CCGCGG C[AC]G^C[GT]G
160 CCGCTG
MvnI 76 CGCG CG^CG
121 CGCG
PleI 414 GACTC GAGTC(4/5)
444 GACTC
PpuMI 1084 AGGTCCC [GA]G^G[AT]CC[CT]
1447 AGGACCT
RcaI 432 TCATGA T^CATGA
1406 TCATGA
RsaI 1433 GTAC GT^AC
1516 GTAC
StyI 387 CCTTGG C^C[AT][AT]GG
1488 CCAAGG
TaqI 167 TCGA T^CGA
738 TCGA
TthHB8I 167 TCGA T^CGA
738 TCGA
XbaI 1120 TCTAGA T^CTAGA
1579 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbsI BbvI BfaI BpuAI
BsaJI BsgI BsiYI BslI BsmFI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII