Restriction map for
pF1KF0040
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccIII AclI AflIII AgeI
Alw44I AocI ApaLI AscI AseI
AsnI AspHI AvaI AviII AvrII
BamHI BbrPI BbvCI BcgI BclI
BlnI BseAI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
ClaI CpoI DraIII Eco81I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MfeI MluI
MroI MslI MunI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 2685 GGTACC G^GTACC
AccII 1563 CGCG CG^CG
AflII 1147 CTTAAG C^TTAAG
AhdI 1654 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3093 GGGCCC GGGCC^C
Asp718I 2685 GGTACC G^GTACC
AspEI 1654 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 3093 GGGCCC G[GA]GC[CT]^C
BbeI 1456 GGCGCC GGCGC^C
BciVI 1045 GGATAC GTATCC(6/5)
BfaI 2044 CTAG C^TAG
BfrI 1147 CTTAAG C^TTAAG
BlpI 814 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 814 GCTCAGC GC^T[ACGT]AGC
BsaAI 679 TACGTG [CT]AC^GT[GA]
BsaBI 2035 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 651 TCATGA T^CATGA
BsrFI 1966 GCCGGC [GA]^CCGG[CT]
BstEII 1222 GGTAACC G^GT[ACGT]ACC
BstPI 1222 GGTAACC G^GT[ACGT]ACC
BstUI 1563 CGCG CG^CG
CelII 814 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1966 GCCGGC [GA]^CCGG[CT]
DraI 673 TTTAAA TTT^AAA
DrdI 2326 GACCAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2262 CGGCCG C^GGCCG
Eam1105I 1654 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2262 CGGCCG C^GGCCG
Eco52I 2262 CGGCCG C^GGCCG
EcoNI 1859 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1222 GGTAACC G^GT[ACGT]ACC
HindIII 847 AAGCTT A^AGCTT
KasI 1456 GGCGCC G^GCGCC
KpnI 2685 GGTACC GGTAC^C
MaeI 2044 CTAG C^TAG
MamI 2035 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1563 CGCG CG^CG
NaeI 1966 GCCGGC GCC^GGC
NarI 1456 GGCGCC GG^CGCC
NgoMIV 1966 GCCGGC G^CCGGC
NspI 2244 GCATGT [GA]CATG^[CT]
PflMI 342 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1753 CTGCAG CTGCA^G
RcaI 651 TCATGA T^CATGA
SfiI 3005 GGCCAGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1456 GGCGCC GGC^GCC
StuI 3013 AGGCCT AGG^CCT
Van91I 342 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2043 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 144 GTCTAC GT^[AC][GT]AC
2638 GTAGAC
AfaI 109 GTAC GT^AC
2686 GTAC
Aor51HI 1907 AGCGCT AGC^GCT
2535 AGCGCT
AspI 1179 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACACTGTC
BalI 2460 TGGCCA TGG^CCA
3004 TGGCCA
BglII 741 AGATCT A^GATCT
2040 AGATCT
BsaI 913 GAGACC GGTCTC(1/5)
1984 GAGACC
BsaWI 771 ACCGGA [AT]^CCGG[AT]
2455 TCCGGT
BsiEI 2262 CGGCCG CG[GA][CT]^CG
3080 CGGTCG
BspMI 2954 ACCTGC ACCTGC(4/8)
2308 GCAGGT
BsrBI 1495 CCGCTC CCGCTC(-3/-3)
2338 CCGCTC
BstBI 1063 TTCGAA TT^CGAA
2394 TTCGAA
BstXI 87 CCAAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2826 CCACTCAGCTGG
Eco47III 1907 AGCGCT AGC^GCT
2535 AGCGCT
EcoRI 2125 GAATTC G^AATTC
2655 GAATTC
MluNI 2460 TGGCCA TGG^CCA
3004 TGGCCA
MscI 2460 TGGCCA TGG^CCA
3004 TGGCCA
NspV 1063 TTCGAA TT^CGAA
2394 TTCGAA
PflFI 1179 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACACTGTC
PpuMI 2081 AGGACCT [GA]G^G[AT]CC[CT]
2417 AGGACCT
PvuII 817 CAGCTG CAG^CTG
2831 CAGCTG
RsaI 109 GTAC GT^AC
2686 GTAC
SapI 184 GAAGAGC GCTCTTC(1/4)
370 GAAGAGC
SfuI 1063 TTCGAA TT^CGAA
2394 TTCGAA
TfiI 1827 GATTC G^A[AT]TC
2392 GATTC
Tth111I 1179 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2101 GACACTGTC
XcmI 800 CCAAAAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1451 CCAAGGGCGCCATGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
AlwNI ApoI Asp700I AvaII BanI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI