Restriction map for
pF1KB3434
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AlwI Aor51HI
ApaI ApoI AseI AsnI Asp700I
AspEI AviII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BpmI BpuAI BsaAI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KspI MfeI MflI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI PacI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tsp509I
TspRI XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 674 GGTACC G^GTACC
AfaI 675 GTAC GT^AC
AflIII 389 ACGCGT A^C[GA][CT]GT
AlwNI 421 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 310 CCTGAGG CC^T[ACGT]AGG
AscI 734 GGCGCGCC GG^CGCGCC
Asp718I 674 GGTACC G^GTACC
AspI 316 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 162 CCTAGG C^CTAGG
BalI 54 TGGCCA TGG^CCA
BbeI 817 GGCGCC GGCGC^C
BcnI 852 CCCGG CC^[GC]GG
BfaI 163 CTAG C^TAG
BlnI 162 CCTAGG C^CTAGG
BlpI 846 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 846 GCTCAGC GC^T[ACGT]AGC
BsaBI 187 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 941 GTCTC GTCTC(1/5)
BsmBI 940 CGTCTC CGTCTC(1/5)
BspMI 677 ACCTGC ACCTGC(4/8)
Bsu36I 310 CCTGAGG CC^T[ACGT]AGG
CelII 846 GCTCAGC GC^T[ACGT]AGC
DpnII 730 GATC ^GATC
DsaI 57 CCATGG C^C[GA][CT]GG
EagI 513 CGGCCG C^GGCCG
EclXI 513 CGGCCG C^GGCCG
Eco52I 513 CGGCCG C^GGCCG
Eco81I 310 CCTGAGG CC^T[ACGT]AGG
EcoNI 477 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 345 GGCCGGCC GGCCGG^CC
HinfI 192 GAATC G^A[ACGT]TC
HphI 799 TCACC GGTGA(8/7)
KasI 817 GGCGCC G^GCGCC
KpnI 674 GGTACC GGTAC^C
MaeI 163 CTAG C^TAG
MaeIII 322 GTCAC ^GT[ACGT]AC
MamI 187 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 730 GATC ^GATC
MluI 389 ACGCGT A^CGCGT
MluNI 54 TGGCCA TGG^CCA
MscI 54 TGGCCA TGG^CCA
MslI 399 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 817 GGCGCC GG^CGCC
NciI 852 CCCGG CC^[GC]GG
NcoI 57 CCATGG C^CATGG
NdeII 730 GATC ^GATC
NlaIII 58 CATG CATG^
PaeR7I 877 CTCGAG C^TCGAG
PflFI 316 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 729 CGATCG CGAT^CG
RsaI 675 GTAC GT^AC
SacI 811 GAGCTC GAGCT^C
Sau3AI 730 GATC ^GATC
SbfI 477 CCTGCAGG CCTGCA^GG
SfoI 817 GGCGCC GGC^GCC
SmlI 877 CTCGAG C^T[CT][GA]AG
Sse8387I 477 CCTGCAGG CCTGCA^GG
TfiI 192 GAATC G^A[AT]TC
Tsp45I 322 GTCAC ^GT[GC]AC
Tth111I 316 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 858 CCAGCCCAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 877 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 817 GGCGCC G[GA]^CG[CT]C
938 GGCGTC
Alw44I 609 GTGCAC G^TGCAC
724 GTGCAC
ApaLI 609 GTGCAC G^TGCAC
724 GTGCAC
BanII 62 GGGCTC G[GA]GC[CT]^C
811 GAGCTC
BglI 341 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
708 GCCCGTGTGGC
BsaHI 817 GGCGCC G[GA]^CG[CT]C
938 GGCGTC
BsiEI 513 CGGCCG CG[GA][CT]^CG
729 CGATCG
BsmFI 920 GGGAC GGGAC(10/14)
34 GTCCC
BsrBI 844 CCGCTC CCGCTC(-3/-3)
118 GAGCGG
BsrFI 346 GCCGGC [GA]^CCGG[CT]
639 GCCGGC
BsrI 224 ACTGG ACTGG(1/-1)
704 ACTGG
BssHII 681 GCGCGC G^CGCGC
735 GCGCGC
BstAPI 366 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
471 GCAAGCCCTGC
BstBI 580 TTCGAA TT^CGAA
757 TTCGAA
Cfr10I 346 GCCGGC [GA]^CCGG[CT]
639 GCCGGC
DraII 131 AGGCCCT [GA]G^G[ACGT]CC[CT]
743 AGGGCCT
EarI 754 CTCTTC CTCTTC(1/4)
253 GAAGAG
EcoO109I 131 AGGCCCT [GA]G^G[ACGT]CC[CT]
743 AGGGCCT
EcoT14I 57 CCATGG C^C[AT][AT]GG
162 CCTAGG
HgaI 355 GACGC GACGC(5/10)
939 GCGTC
Hin1I 817 GGCGCC G[GA]^CG[CT]C
938 GGCGTC
Ksp632I 754 CTCTTC CTCTTC(1/4)
253 GAAGAG
MaeII 377 ACGT A^CGT
950 ACGT
NaeI 346 GCCGGC GCC^GGC
639 GCCGGC
NgoMIV 346 GCCGGC G^CCGGC
639 GCCGGC
NspV 580 TTCGAA TT^CGAA
757 TTCGAA
PflMI 39 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCAGCAGATGG
PstI 151 CTGCAG CTGCA^G
478 CTGCAG
PvuII 370 CAGCTG CAG^CTG
433 CAGCTG
SfcI 151 CTGCAG C^T[GA][CT]AG
478 CTGCAG
SfuI 580 TTCGAA TT^CGAA
757 TTCGAA
StuI 360 AGGCCT AGG^CCT
896 AGGCCT
StyI 57 CCATGG C^C[AT][AT]GG
162 CCTAGG
Van91I 39 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCAGCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BbvI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIV
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I