Restriction map for
pF1KB4155
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRV EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I HindIII KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Alw44I 91 GTGCAC G^TGCAC
AlwNI 608 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 91 GTGCAC G^TGCAC
AspI 879 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 156 CTCGGG C^[CT]CG[GA]G
AvaII 252 GGACC G^G[AT]CC
AviII 680 TGCGCA TGC^GCA
BbsI 304 GAAGAC GAAGAC(2/6)
BbvI 721 GCAGC GCAGC(8/12)
BfaI 848 CTAG C^TAG
BpmI 620 CTGGAG CTGGAG(16/14)
BpuAI 304 GAAGAC GAAGAC(2/6)
BsaI 330 GGTCTC GGTCTC(1/5)
BseRI 420 CTCCTC GAGGAG(10/8)
BsoBI 156 CTCGGG C^[CT]CG[GA]G
BsrBI 697 GAGCGG CCGCTC(-3/-3)
BstEII 258 GGTGACC G^GT[ACGT]ACC
BstPI 258 GGTGACC G^GT[ACGT]ACC
Cac8I 842 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 252 GGACC G^G[ACGT]CC
EcoO65I 258 GGTGACC G^GT[ACGT]ACC
EcoT14I 195 CCAAGG C^C[AT][AT]GG
FspI 680 TGCGCA TGC^GCA
HaeIII 790 GGCC GG^CC
HincII 409 GTTAAC GT[CT]^[GA]AC
HindII 409 GTTAAC GT[CT]^[GA]AC
HpaI 409 GTTAAC GTT^AAC
MaeI 848 CTAG C^TAG
MseI 410 TTAA T^TAA
MslI 321 CACTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 630 CAGCTG C[AC]G^C[GT]G
NspI 454 ACATGC [GA]CATG^[CT]
PflFI 879 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 212 GACTC GAGTC(4/5)
PvuII 630 CAGCTG CAG^CTG
Sau96I 252 GGACC G^G[ACGT]CC
SexAI 493 ACCTGGT A^CC[AT]GGT
StyI 195 CCAAGG C^C[AT][AT]GG
Tru9I 410 TTAA T^TAA
TseI 721 GCAGC G^C[AT]GC
Tth111I 879 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 344 GCGG CCGC(-3/-1)
699 GCGG
AcsI 116 GAATTC [GA]^AATT[CT]
868 GAATTC
AlwI 54 GATCC GGATC(4/5)
598 GATCC
ApoI 116 GAATTC [GA]^AATT[CT]
868 GAATTC
AspHI 63 GTGCTC G[AT]GC[AT]^C
91 GTGCAC
BcnI 255 CCGGG CC^[GC]GG
441 CCGGG
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
91 GTGCAC
BsmAI 200 GAGAC GTCTC(1/5)
331 GTCTC
BstF5I 395 CATCC GGATG(2/0)
414 CATCC
CfoI 13 GCGC GCG^C
681 GCGC
EcoRI 116 GAATTC G^AATTC
868 GAATTC
Fnu4HI 344 GCGGC GC^[ACGT]GC
721 GCAGC
FokI 395 CATCC GGATG(9/13)
414 CATCC
HapII 255 CCGG C^CGG
441 CCGG
HhaI 13 GCGC GCG^C
681 GCGC
HinP1I 13 GCGC G^CGC
681 GCGC
HpaII 255 CCGG C^CGG
441 CCGG
ItaI 344 GCGGC GC^[ACGT]GC
721 GCAGC
MspI 255 CCGG C^CGG
441 CCGG
MwoI 344 GCGGCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
375 GCTCATCGTGC
NciI 255 CCGGG CC^[GC]GG
441 CCGGG
NlaIV 437 GGAACC GG[ACGT]^[ACGT]CC
472 GGTTCC
SfaNI 516 GATGC GCATC(5/9)
767 GCATC
SfcI 404 CTACAG C^T[GA][CT]AG
780 CTATAG
TaqI 20 TCGA T^CGA
820 TCGA
Tsp45I 259 GTGAC ^GT[GC]AC
295 GTGAC
Tsp509I 117 AATT ^AATT
869 AATT
TthHB8I 20 TCGA T^CGA
820 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
DdeI DpnII EarI EcoRII HinfI
HphI Ksp632I MaeIII MboI MboII
MnlI MvaI NdeII NlaIII Sau3AI
ScrFI TfiI TspRI