Restriction map for
pF1KB4203
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MseI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 661 GACGTC GACGT^C
AccI 265 GTCGAC GT^[AC][GT]AC
AcyI 661 GACGTC G[GA]^CG[CT]C
AflIII 317 ACGTGT A^C[GA][CT]GT
AocI 828 CCTGAGG CC^T[ACGT]AGG
ApaI 437 GGGCCC GGGCC^C
AvaI 799 CCCGAG C^[CT]CG[GA]G
AviII 257 TGCGCA TGC^GCA
BamHI 194 GGATCC G^GATCC
BanI 172 GGCACC G^G[CT][GA]CC
BsaBI 693 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 661 GACGTC G[GA]^CG[CT]C
BsaWI 365 ACCGGA [AT]^CCGG[AT]
BseRI 760 GAGGAG GAGGAG(10/8)
BsgI 451 CTGCAC GTGCAG(16/14)
BsmBI 314 GAGACG CGTCTC(1/5)
BsoBI 799 CCCGAG C^[CT]CG[GA]G
BspMI 750 GCAGGT ACCTGC(4/8)
BsrBI 168 GAGCGG CCGCTC(-3/-3)
BsrDI 915 CATTGC GCAATG(2/0)
BsrFI 586 GCCGGC [GA]^CCGG[CT]
BsrI 120 ACTGG ACTGG(1/-1)
BstEII 105 GGTGACC G^GT[ACGT]ACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
BstXI 572 CCAAGACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 828 CCTGAGG CC^T[ACGT]AGG
CfoI 258 GCGC GCG^C
Cfr10I 586 GCCGGC [GA]^CCGG[CT]
DsaI 126 CCGTGG C^C[GA][CT]GG
Eco81I 828 CCTGAGG CC^T[ACGT]AGG
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
FspI 257 TGCGCA TGC^GCA
HhaI 258 GCGC GCG^C
Hin1I 661 GACGTC G[GA]^CG[CT]C
HinP1I 258 GCGC G^CGC
HincII 265 GTCGAC GT[CT]^[GA]AC
HindII 265 GTCGAC GT[CT]^[GA]AC
MamI 693 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 742 CAGCTG C[AC]G^C[GT]G
NaeI 586 GCCGGC GCC^GGC
NgoMIV 586 GCCGGC G^CCGGC
NspI 254 ACATGC [GA]CATG^[CT]
PpuMI 727 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 742 CAGCTG CAG^CTG
SacI 334 GAGCTC GAGCT^C
SalI 265 GTCGAC G^TCGAC
TaqI 266 TCGA T^CGA
Tsp509I 393 AATT ^AATT
TthHB8I 266 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 739 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
823 CAGTGCCTG
AspHI 334 GAGCTC G[AT]GC[AT]^C
874 GAGCAC
BalI 569 TGGCCA TGG^CCA
695 TGGCCA
BanII 334 GAGCTC G[GA]GC[CT]^C
437 GGGCCC
BbsI 906 GAAGAC GAAGAC(2/6)
946 GAAGAC
BpuAI 906 GAAGAC GAAGAC(2/6)
946 GAAGAC
BsiEI 263 CGGTCG CG[GA][CT]^CG
786 CGACCG
BsiHKAI 334 GAGCTC G[AT]GC[AT]^C
874 GAGCAC
BsmAI 71 GAGAC GTCTC(1/5)
314 GAGAC
BsmFI 727 GGGAC GGGAC(10/14)
768 GTCCC
DraII 437 GGGCCCT [GA]G^G[ACGT]CC[CT]
727 GGGACCC
EcoO109I 437 GGGCCCT [GA]G^G[ACGT]CC[CT]
727 GGGACCC
EcoT14I 350 CCTTGG C^C[AT][AT]GG
408 CCTTGG
MaeII 317 ACGT A^CGT
662 ACGT
MluNI 569 TGGCCA TGG^CCA
695 TGGCCA
MscI 569 TGGCCA TGG^CCA
695 TGGCCA
StyI 350 CCTTGG C^C[AT][AT]GG
408 CCTTGG
XcmI 110 CCATCTCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
683 CCATGATTGAGATGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI XhoII