Restriction map for
pF1KB5771
##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI Aor51HI ApaI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BsaHI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI BstBI BstUI CelII ClaI
CpoI DraI DraIII DsaI Eco47III
EcoRV EcoT22I FbaI FseI HaeII
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 937 GGTACC G^GTACC
AcsI 319 GAATTC [GA]^AATT[CT]
AflII 714 CTTAAG C^TTAAG
AhdI 1528 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 973 GTGCAC G^TGCAC
AlwI 1757 GGATC GGATC(4/5)
AocI 2099 CCTCAGG CC^T[ACGT]AGG
ApaLI 973 GTGCAC G^TGCAC
ApoI 319 GAATTC [GA]^AATT[CT]
Asp718I 937 GGTACC G^GTACC
AspEI 1528 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1001 TGCGCA TGC^GCA
BalI 983 TGGCCA TGG^CCA
BfrI 714 CTTAAG C^TTAAG
BsaBI 861 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1754 ACCGGA [AT]^CCGG[AT]
BsiEI 1710 CGGCCG CG[GA][CT]^CG
BspHI 2124 TCATGA T^CATGA
BsrBI 478 GAGCGG CCGCTC(-3/-3)
Bst1107I 747 GTATAC GTA^TAC
BstEII 1879 GGTCACC G^GT[ACGT]ACC
BstPI 1879 GGTCACC G^GT[ACGT]ACC
BstZ17I 747 GTATAC GTA^TAC
Bsu36I 2099 CCTCAGG CC^T[ACGT]AGG
DrdI 1159 GACAGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1710 CGGCCG C^GGCCG
Eam1105I 1528 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1710 CGGCCG C^GGCCG
Eco52I 1710 CGGCCG C^GGCCG
Eco81I 2099 CCTCAGG CC^T[ACGT]AGG
EcoNI 1182 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1879 GGTCACC G^GT[ACGT]ACC
EcoRI 319 GAATTC G^AATTC
FspI 1001 TGCGCA TGC^GCA
HincII 798 GTTGAC GT[CT]^[GA]AC
HindII 798 GTTGAC GT[CT]^[GA]AC
KpnI 937 GGTACC GGTAC^C
MamI 861 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 983 TGGCCA TGG^CCA
MscI 983 TGGCCA TGG^CCA
NheI 1101 GCTAGC G^CTAGC
PstI 1450 CTGCAG CTGCA^G
RcaI 2124 TCATGA T^CATGA
SacI 2085 GAGCTC GAGCT^C
SbfI 1449 CCTGCAGG CCTGCA^GG
ScaI 2057 AGTACT AGT^ACT
SfcI 1450 CTGCAG C^T[GA][CT]AG
SmlI 714 CTTAAG C^T[CT][GA]AG
Sse8387I 1449 CCTGCAGG CCTGCA^GG
TfiI 2110 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 747 GTATAC GT^[AC][GT]AC
919 GTCTAC
AlwNI 1016 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1899 CAGTAACTG
BanII 2085 GAGCTC G[GA]GC[CT]^C
2143 GAGCCC
BfaI 62 CTAG C^TAG
1102 CTAG
BglI 1068 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1464 GCCCCCAGGGC
BglII 659 AGATCT A^GATCT
1808 AGATCT
BsaAI 750 TACGTG [CT]AC^GT[GA]
2091 TACGTG
Bsp1407I 569 TGTACA T^GTACA
1919 TGTACA
BsrGI 569 TGTACA T^GTACA
1919 TGTACA
BstAPI 228 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1944 GCATGCAGTGC
EcoT14I 1427 CCAAGG C^C[AT][AT]GG
1523 CCAAGG
HgaI 736 GACGC GACGC(5/10)
1288 GACGC
MaeI 62 CTAG C^TAG
1102 CTAG
MseI 680 TTAA T^TAA
715 TTAA
NaeI 237 GCCGGC GCC^GGC
828 GCCGGC
NgoMIV 237 GCCGGC G^CCGGC
828 GCCGGC
NspI 228 GCATGC [GA]CATG^[CT]
1944 GCATGC
PpuMI 277 GGGACCC [GA]G^G[AT]CC[CT]
1526 AGGACCT
PvuII 463 CAGCTG CAG^CTG
1978 CAGCTG
SexAI 1529 ACCTGGT A^CC[AT]GGT
1547 ACCAGGT
SphI 228 GCATGC GCATG^C
1944 GCATGC
SspBI 569 TGTACA T^GTACA
1919 TGTACA
StyI 1427 CCAAGG C^C[AT][AT]GG
1523 CCAAGG
TaqI 611 TCGA T^CGA
1685 TCGA
Tru9I 680 TTAA T^TAA
715 TTAA
TthHB8I 611 TCGA T^CGA
1685 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI
XcmI XhoII