Restriction map for
pF1KB5487
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiWI BsmAI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HgaI
HindIII HpaI KpnI MamI MfeI
MflI MluI MroI MslI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 568 GTCTAC GT^[AC][GT]AC
AflIII 17 ACATGT A^C[GA][CT]GT
Alw44I 21 GTGCAC G^TGCAC
ApaLI 21 GTGCAC G^TGCAC
AspHI 21 GTGCAC G[AT]GC[AT]^C
AvaI 631 CCCGAG C^[CT]CG[GA]G
BalI 449 TGGCCA TGG^CCA
BbrPI 403 CACGTG CAC^GTG
BbvCI 490 CCTCAGC CCTCAGC(-5/-2)
BpmI 263 CTCCAG CTGGAG(16/14)
BsaAI 403 CACGTG [CT]AC^GT[GA]
BsaWI 152 TCCGGT [AT]^CCGG[AT]
BseRI 693 GAGGAG GAGGAG(10/8)
BsgI 406 GTGCAG GTGCAG(16/14)
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsiHKAI 21 GTGCAC G[AT]GC[AT]^C
BsmI 320 GCATTC GAATGC(1/-1)
BsoBI 631 CCCGAG C^[CT]CG[GA]G
BspLU11I 17 ACATGT A^CATGT
BspMI 196 GCAGGT ACCTGC(4/8)
BsrFI 85 GCCGGC [GA]^CCGG[CT]
BsrI 144 ACTGG ACTGG(1/-1)
BstXI 122 CCAGCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 85 GCCGGC [GA]^CCGG[CT]
DraII 683 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 87 CGGCCG C^GGCCG
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
EcoO109I 683 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 40 CCCGC CCCGC(4/6)
HincII 484 GTTGAC GT[CT]^[GA]AC
HindII 484 GTTGAC GT[CT]^[GA]AC
HinfI 170 GATTC G^A[ACGT]TC
KspI 56 CCGCGG CCGC^GG
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
MseI 701 TTAA T^TAA
NaeI 85 GCCGGC GCC^GGC
NgoMIV 85 GCCGGC G^CCGGC
NheI 507 GCTAGC G^CTAGC
PmaCI 403 CACGTG CAC^GTG
PmlI 403 CACGTG CAC^GTG
PpuMI 683 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 307 CAGCTG CAG^CTG
SacII 56 CCGCGG CCGC^GG
SapI 244 GCTCTTC GCTCTTC(1/4)
SfaNI 334 GATGC GCATC(5/9)
SphI 515 GCATGC GCATG^C
TfiI 170 GATTC G^A[AT]TC
Tru9I 701 TTAA T^TAA
Tsp509I 556 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 42 CGCG CG^CG
57 CGCG
AcyI 79 GGCGCC G[GA]^CG[CT]C
118 GGCGCC
AfaI 36 GTAC GT^AC
626 GTAC
AlwNI 332 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGGACCTG
BbeI 79 GGCGCC GGCGC^C
118 GGCGCC
BcnI 46 CCGGG CC^[GC]GG
428 CCCGG
BmyI 21 GTGCAC G[AGT]GC[ACT]^C
423 GGGCAC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
118 GGCGCC
Bsp1286I 21 GTGCAC G[AGT]GC[ACT]^C
423 GGGCAC
BstUI 42 CGCG CG^CG
57 CGCG
DdeI 292 CTGAG C^T[ACGT]AG
491 CTCAG
DpnII 105 GATC ^GATC
345 GATC
DsaI 56 CCGCGG C^C[GA][CT]GG
110 CCGTGG
EaeI 87 CGGCCG [CT]^GGCC[GA]
449 TGGCCA
EarI 245 CTCTTC CTCTTC(1/4)
690 GAAGAG
Hin1I 79 GGCGCC G[GA]^CG[CT]C
118 GGCGCC
KasI 79 GGCGCC G^GCGCC
118 GGCGCC
Ksp632I 245 CTCTTC CTCTTC(1/4)
690 GAAGAG
MaeII 404 ACGT A^CGT
584 ACGT
MboI 105 GATC ^GATC
345 GATC
MvnI 42 CGCG CG^CG
57 CGCG
NarI 79 GGCGCC GG^CGCC
118 GGCGCC
NciI 46 CCGGG CC^[GC]GG
428 CCCGG
NdeII 105 GATC ^GATC
345 GATC
PstI 297 CTGCAG CTGCA^G
502 CTGCAG
RsaI 36 GTAC GT^AC
626 GTAC
Sau3AI 105 GATC ^GATC
345 GATC
SfoI 79 GGCGCC GGC^GCC
118 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BfaI BsaJI BsiYI BslI BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NlaIII NlaIV NspI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI