################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeI MaeIII MfeI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 275 ACGCGT A^C[GA][CT]GT AvaI 309 CTCGGG C^[CT]CG[GA]G BanI 304 GGCGCC G^G[CT][GA]CC BanII 517 GGGCTC G[GA]GC[CT]^C BbeI 304 GGCGCC GGCGC^C BbrPI 118 CACGTG CAC^GTG BbsI 576 GAAGAC GAAGAC(2/6) BcnI 561 CCGGG CC^[GC]GG BpmI 595 CTGGAG CTGGAG(16/14) BpuAI 576 GAAGAC GAAGAC(2/6) BsaBI 57 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 444 GGTCTC GGTCTC(1/5) BsmAI 445 GTCTC GTCTC(1/5) BsoBI 309 CTCGGG C^[CT]CG[GA]G BspMI 105 GCAGGT ACCTGC(4/8) BssSI 507 CTCGTG CACGAG(-5/-1) BstAPI 6 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 56 AGATCC [GA]^GATC[CT] Cfr13I 191 GGCCC G^G[ACGT]CC DraIII 7 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 581 CCATGG C^C[GA][CT]GG FauI 160 GCGGG CCCGC(4/6) HapII 561 CCGG C^CGG HpaII 561 CCGG C^CGG KasI 304 GGCGCC G^GCGCC MamI 57 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 56 AGATCC [GA]^GATC[CT] MluI 275 ACGCGT A^CGCGT MspI 561 CCGG C^CGG NarI 304 GGCGCC GG^CGCC NciI 561 CCGGG CC^[GC]GG NcoI 581 CCATGG C^CATGG NlaIII 582 CATG CATG^ PmaCI 118 CACGTG CAC^GTG PmlI 118 CACGTG CAC^GTG Sau96I 191 GGCCC G^G[ACGT]CC SexAI 440 ACCAGGT A^CC[AT]GGT SfoI 304 GGCGCC GGC^GCC TspRI 7 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 441 CCAGGTCTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 56 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 304 GGCGCC G[GA]^CG[CT]C 384 GGCGTC AfaI 426 GTAC GT^AC 546 GTAC AlwI 27 GATCC GGATC(4/5) 57 GATCC AlwNI 37 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 142 CAGGAGCTG Aor51HI 236 AGCGCT AGC^GCT 356 AGCGCT BsaAI 118 CACGTG [CT]AC^GT[GA] 423 CACGTA BsaHI 304 GGCGCC G[GA]^CG[CT]C 384 GGCGTC BsgI 4 CTGCAC GTGCAG(16/14) 13 GTGCAG BsmFI 387 GTCCC GGGAC(10/14) 551 GTCCC BssHII 18 GCGCGC G^CGCGC 20 GCGCGC Eco47III 236 AGCGCT AGC^GCT 356 AGCGCT HgaI 277 GCGTC GACGC(5/10) 385 GCGTC Hin1I 304 GGCGCC G[GA]^CG[CT]C 384 GGCGTC MaeII 119 ACGT A^CGT 424 ACGT MspA1I 158 CAGCGG C[AC]G^C[GT]G 168 CAGCGG NlaIV 304 GGCGCC GG[ACGT]^[ACGT]CC 497 GGTTCC RsaI 426 GTAC GT^AC 546 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI DpnII EcoRII EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I ItaI MboI MboII MnlI MvaI MvnI MwoI NdeII PflMI Sau3AI ScrFI StyI TaqI TseI TthHB8I Van91I