################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI Ksp632I KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StyI TfiI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 725 GGTACC G^GTACC AccI 349 GTCGAC GT^[AC][GT]AC AccIII 68 TCCGGA T^CCGGA AcsI 838 AAATTT [GA]^AATT[CT] AlwNI 656 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 838 AAATTT [GA]^AATT[CT] Asp718I 725 GGTACC G^GTACC AspHI 217 GTGCTC G[AT]GC[AT]^C BanII 64 GGGCTC G[GA]GC[CT]^C BcnI 402 CCCGG CC^[GC]GG BglII 171 AGATCT A^GATCT BsaWI 68 TCCGGA [AT]^CCGG[AT] BseAI 68 TCCGGA T^CCGGA BseRI 121 CTCCTC GAGGAG(10/8) BsgI 406 GTGCAG GTGCAG(16/14) BsiHKAI 217 GTGCTC G[AT]GC[AT]^C BsmFI 55 GTCCC GGGAC(10/14) BspEI 68 TCCGGA T^CCGGA BstBI 806 TTCGAA TT^CGAA BstYI 171 AGATCT [GA]^GATC[CT] DpnII 172 GATC ^GATC DraI 841 TTTAAA TTT^AAA DraIII 279 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 72 GATATC GAT^ATC HgaI 337 GACGC GACGC(5/10) HinfI 237 GACTC G^A[ACGT]TC KpnI 725 GGTACC GGTAC^C MboI 172 GATC ^GATC MflI 171 AGATCT [GA]^GATC[CT] MroI 68 TCCGGA T^CCGGA MseI 842 TTAA T^TAA MslI 92 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 402 CCCGG CC^[GC]GG NdeII 172 GATC ^GATC NspI 158 GCATGC [GA]CATG^[CT] NspV 806 TTCGAA TT^CGAA PflMI 278 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 237 GACTC GAGTC(4/5) PpuMI 854 AGGACCC [GA]G^G[AT]CC[CT] PstI 621 CTGCAG CTGCA^G SalI 349 GTCGAC G^TCGAC Sau3AI 172 GATC ^GATC SfaNI 104 GCATC GCATC(5/9) SfcI 621 CTGCAG C^T[GA][CT]AG SfuI 806 TTCGAA TT^CGAA SphI 158 GCATGC GCATG^C StuI 317 AGGCCT AGG^CCT SwaI 840 ATTTAAAT ATTT^AAAT Tru9I 842 TTAA T^TAA Van91I 278 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 171 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 162 GCGG CCGC(-3/-1) 608 GCGG AocI 265 CCTGAGG CC^T[ACGT]AGG 483 CCTGAGG AvaI 220 CTCGAG C^[CT]CG[GA]G 321 CTCGAG BanI 371 GGTGCC G^G[CT][GA]CC 725 GGTACC BmyI 64 GGGCTC G[AGT]GC[ACT]^C 217 GTGCTC BsoBI 220 CTCGAG C^[CT]CG[GA]G 321 CTCGAG Bsp1286I 64 GGGCTC G[AGT]GC[ACT]^C 217 GTGCTC BsrI 824 ACTGG ACTGG(1/-1) 96 CCAGT Bsu36I 265 CCTGAGG CC^T[ACGT]AGG 483 CCTGAGG DraII 443 AGGCCCC [GA]G^G[ACGT]CC[CT] 854 AGGACCC Eco81I 265 CCTGAGG CC^T[ACGT]AGG 483 CCTGAGG EcoO109I 443 AGGCCCC [GA]G^G[ACGT]CC[CT] 854 AGGACCC HaeIII 318 GGCC GG^CC 444 GGCC HapII 69 CCGG C^CGG 403 CCGG HincII 349 GTCGAC GT[CT]^[GA]AC 696 GTCAAC HindII 349 GTCGAC GT[CT]^[GA]AC 696 GTCAAC HpaII 69 CCGG C^CGG 403 CCGG MspI 69 CCGG C^CGG 403 CCGG NlaIII 108 CATG CATG^ 159 CATG PaeR7I 220 CTCGAG C^TCGAG 321 CTCGAG XhoI 220 CTCGAG C^TCGAG 321 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HphI ItaI MaeI MaeIII MboII MnlI MspA1I MvaI NlaIV PvuII RsaI Sau96I ScrFI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I