Restriction map for
pF1KB6347
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 482 TCCGGA T^CCGGA
AfaI 225 GTAC GT^AC
AflII 118 CTTAAG C^TTAAG
AlwI 528 GATCC GGATC(4/5)
AocI 376 CCTCAGG CC^T[ACGT]AGG
Asp700I 571 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 489 TGGCCA TGG^CCA
BanII 66 GAGCTC G[GA]GC[CT]^C
BbsI 501 GAAGAC GAAGAC(2/6)
BciVI 390 GTATCC GTATCC(6/5)
BfaI 568 CTAG C^TAG
BfrI 118 CTTAAG C^TTAAG
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BpuAI 501 GAAGAC GAAGAC(2/6)
BsaWI 482 TCCGGA [AT]^CCGG[AT]
BseAI 482 TCCGGA T^CCGGA
BseRI 190 GAGGAG GAGGAG(10/8)
BsmAI 539 GAGAC GTCTC(1/5)
BspEI 482 TCCGGA T^CCGGA
BsrBI 151 GAGCGG CCGCTC(-3/-3)
BstF5I 373 CATCC GGATG(2/0)
Bsu36I 376 CCTCAGG CC^T[ACGT]AGG
Cac8I 713 GCCTGC GC[ACGT]^[ACGT]GC
CelII 63 GCTGAGC GC^T[ACGT]AGC
CfoI 201 GCGC GCG^C
DpnII 528 GATC ^GATC
DraII 281 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 489 TGGCCA [CT]^GGCC[GA]
Eco81I 376 CCTCAGG CC^T[ACGT]AGG
EcoO109I 281 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 723 CCTTGG C^C[AT][AT]GG
FokI 373 CATCC GGATG(9/13)
HhaI 201 GCGC GCG^C
HinP1I 201 GCGC G^CGC
HincII 42 GTTGAC GT[CT]^[GA]AC
HindII 42 GTTGAC GT[CT]^[GA]AC
MaeI 568 CTAG C^TAG
MboI 528 GATC ^GATC
MluNI 489 TGGCCA TGG^CCA
MroI 482 TCCGGA T^CCGGA
MscI 489 TGGCCA TGG^CCA
MseI 119 TTAA T^TAA
MspA1I 361 CAGCGG C[AC]G^C[GT]G
NdeII 528 GATC ^GATC
PflMI 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 281 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 225 GTAC GT^AC
SacI 66 GAGCTC GAGCT^C
SapI 717 GCTCTTC GCTCTTC(1/4)
Sau3AI 528 GATC ^GATC
SmlI 118 CTTAAG C^T[CT][GA]AG
StuI 522 AGGCCT AGG^CCT
StyI 723 CCTTGG C^C[AT][AT]GG
TaqI 206 TCGA T^CGA
Tru9I 119 TTAA T^TAA
Tsp509I 316 AATT ^AATT
TthHB8I 206 TCGA T^CGA
Van91I 552 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 571 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 406 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
553 CAGACCCTG
AvaII 282 GGACC G^G[AT]CC
545 GGACC
BcnI 228 CCGGG CC^[GC]GG
731 CCGGG
BsgI 668 CTGCAC GTGCAG(16/14)
358 GTGCAG
BsmFI 89 GGGAC GGGAC(10/14)
145 GGGAC
EarI 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
FauI 153 GCGGG CCCGC(4/6)
300 GCGGG
Ksp632I 718 CTCTTC CTCTTC(1/4)
496 GAAGAG
MaeII 102 ACGT A^CGT
215 ACGT
NciI 228 CCGGG CC^[GC]GG
731 CCGGG
PstI 321 CTGCAG CTGCA^G
412 CTGCAG
SfaNI 564 GATGC GCATC(5/9)
681 GATGC
SfcI 321 CTGCAG C^T[GA][CT]AG
412 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NlaIV PleI Sau96I ScrFI TfiI
TseI Tsp45I TspRI