################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AcsI 93 AAATTT [GA]^AATT[CT] ApoI 93 AAATTT [GA]^AATT[CT] BbsI 79 GAAGAC GAAGAC(2/6) BbvCI 669 GCTGAGG CCTCAGC(-5/-2) BcnI 734 CCCGG CC^[GC]GG BfaI 539 CTAG C^TAG BpuAI 79 GAAGAC GAAGAC(2/6) BsmAI 751 GAGAC GTCTC(1/5) BsrBI 58 GAGCGG CCGCTC(-3/-3) BsrDI 743 GCAATG GCAATG(2/0) BstNI 434 CCAGG CC^[AT]GG DrdI 233 GACTGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 434 CCAGG ^CC[AT]GG HphI 215 TCACC GGTGA(8/7) MaeI 539 CTAG C^TAG MseI 148 TTAA T^TAA MspA1I 340 CAGCTG C[AC]G^C[GT]G MvaI 434 CCAGG CC^[AT]GG NciI 734 CCCGG CC^[GC]GG PflMI 336 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 753 GACTC GAGTC(4/5) PvuII 340 CAGCTG CAG^CTG SmlI 37 CTCAAG C^T[CT][GA]AG TaqI 716 TCGA T^CGA Tru9I 148 TTAA T^TAA TthHB8I 716 TCGA T^CGA Van91I 336 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 538 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 609 CCGC CCGC(-3/-1) 60 GCGG AfaI 418 GTAC GT^AC 759 GTAC AvaII 241 GGTCC G^G[AT]CC 528 GGACC BsiYI 336 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 591 CCATGTGGAGG BslI 336 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 591 CCATGTGGAGG BsmFI 196 GTCCC GGGAC(10/14) 242 GTCCC BsrI 234 ACTGG ACTGG(1/-1) 193 CCAGT BssKI 434 CCAGG ^CC[ACGT]GG 734 CCCGG BstYI 170 AGATCC [GA]^GATC[CT] 374 AGATCC EcoT14I 288 CCAAGG C^C[AT][AT]GG 296 CCAAGG HaeIII 3 GGCC GG^CC 731 GGCC HapII 378 CCGG C^CGG 735 CCGG HpaII 378 CCGG C^CGG 735 CCGG MflI 170 AGATCC [GA]^GATC[CT] 374 AGATCC MspI 378 CCGG C^CGG 735 CCGG RsaI 418 GTAC GT^AC 759 GTAC ScrFI 434 CCAGG CC^[ACGT]GG 734 CCCGG StyI 288 CCAAGG C^C[AT][AT]GG 296 CCAAGG Tsp45I 616 GTGAC ^GT[GC]AC 685 GTCAC Tsp509I 94 AATT ^AATT 563 AATT XhoII 170 AGATCC [GA]^GATC[CT] 374 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI AlwI AlwNI BbvI BsaJI BstF5I Cfr13I DdeI DpnII Fnu4HI FokI HinfI ItaI MaeIII MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI Sau96I SfaNI TfiI TseI