Restriction map for pF1KB6359
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TspRI       Tth111I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AcsI           93  AAATTT               [GA]^AATT[CT]
ApoI           93  AAATTT               [GA]^AATT[CT]
BbsI           79  GAAGAC               GAAGAC(2/6)
BbvCI         669  GCTGAGG              CCTCAGC(-5/-2)
BcnI          734  CCCGG                CC^[GC]GG
BfaI          539  CTAG                 C^TAG
BpuAI          79  GAAGAC               GAAGAC(2/6)
BsmAI         751  GAGAC                GTCTC(1/5)
BsrBI          58  GAGCGG               CCGCTC(-3/-3)
BsrDI         743  GCAATG               GCAATG(2/0)
BstNI         434  CCAGG                CC^[AT]GG
DrdI          233  GACTGGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        434  CCAGG                ^CC[AT]GG
HphI          215  TCACC                GGTGA(8/7)
MaeI          539  CTAG                 C^TAG
MseI          148  TTAA                 T^TAA
MspA1I        340  CAGCTG               C[AC]G^C[GT]G
MvaI          434  CCAGG                CC^[AT]GG
NciI          734  CCCGG                CC^[GC]GG
PflMI         336  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          753  GACTC                GAGTC(4/5)
PvuII         340  CAGCTG               CAG^CTG
SmlI           37  CTCAAG               C^T[CT][GA]AG
TaqI          716  TCGA                 T^CGA
Tru9I         148  TTAA                 T^TAA
TthHB8I       716  TCGA                 T^CGA
Van91I        336  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          538  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          609  CCGC                 CCGC(-3/-1)
               60  GCGG                
AfaI          418  GTAC                 GT^AC
              759  GTAC                
AvaII         241  GGTCC                G^G[AT]CC
              528  GGACC               
BsiYI         336  CCAGCAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              591  CCATGTGGAGG         
BslI          336  CCAGCAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              591  CCATGTGGAGG         
BsmFI         196  GTCCC                GGGAC(10/14)
              242  GTCCC               
BsrI          234  ACTGG                ACTGG(1/-1)
              193  CCAGT               
BssKI         434  CCAGG                ^CC[ACGT]GG
              734  CCCGG               
BstYI         170  AGATCC               [GA]^GATC[CT]
              374  AGATCC              
EcoT14I       288  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
HaeIII          3  GGCC                 GG^CC
              731  GGCC                
HapII         378  CCGG                 C^CGG
              735  CCGG                
HpaII         378  CCGG                 C^CGG
              735  CCGG                
MflI          170  AGATCC               [GA]^GATC[CT]
              374  AGATCC              
MspI          378  CCGG                 C^CGG
              735  CCGG                
RsaI          418  GTAC                 GT^AC
              759  GTAC                
ScrFI         434  CCAGG                CC^[ACGT]GG
              734  CCCGG               
StyI          288  CCAAGG               C^C[AT][AT]GG
              296  CCAAGG              
Tsp45I        616  GTGAC                ^GT[GC]AC
              685  GTCAC               
Tsp509I        94  AATT                 ^AATT
              563  AATT                
XhoII         170  AGATCC               [GA]^GATC[CT]
              374  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        AlwI        AlwNI       BbvI        BsaJI       
BstF5I      Cfr13I      DdeI        DpnII       Fnu4HI      
FokI        HinfI       ItaI        MaeIII      MboI        
MboII       MnlI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      SfaNI       TfiI        TseI        
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