##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TspRI Tth111I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 93 AAATTT [GA]^AATT[CT]
ApoI 93 AAATTT [GA]^AATT[CT]
BbsI 79 GAAGAC GAAGAC(2/6)
BbvCI 669 GCTGAGG CCTCAGC(-5/-2)
BcnI 734 CCCGG CC^[GC]GG
BfaI 539 CTAG C^TAG
BpuAI 79 GAAGAC GAAGAC(2/6)
BsmAI 751 GAGAC GTCTC(1/5)
BsrBI 58 GAGCGG CCGCTC(-3/-3)
BsrDI 743 GCAATG GCAATG(2/0)
BstNI 434 CCAGG CC^[AT]GG
DrdI 233 GACTGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 434 CCAGG ^CC[AT]GG
HphI 215 TCACC GGTGA(8/7)
MaeI 539 CTAG C^TAG
MseI 148 TTAA T^TAA
MspA1I 340 CAGCTG C[AC]G^C[GT]G
MvaI 434 CCAGG CC^[AT]GG
NciI 734 CCCGG CC^[GC]GG
PflMI 336 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 753 GACTC GAGTC(4/5)
PvuII 340 CAGCTG CAG^CTG
SmlI 37 CTCAAG C^T[CT][GA]AG
TaqI 716 TCGA T^CGA
Tru9I 148 TTAA T^TAA
TthHB8I 716 TCGA T^CGA
Van91I 336 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 538 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 609 CCGC CCGC(-3/-1)
60 GCGG
AfaI 418 GTAC GT^AC
759 GTAC
AvaII 241 GGTCC G^G[AT]CC
528 GGACC
BsiYI 336 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
591 CCATGTGGAGG
BslI 336 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
591 CCATGTGGAGG
BsmFI 196 GTCCC GGGAC(10/14)
242 GTCCC
BsrI 234 ACTGG ACTGG(1/-1)
193 CCAGT
BssKI 434 CCAGG ^CC[ACGT]GG
734 CCCGG
BstYI 170 AGATCC [GA]^GATC[CT]
374 AGATCC
EcoT14I 288 CCAAGG C^C[AT][AT]GG
296 CCAAGG
HaeIII 3 GGCC GG^CC
731 GGCC
HapII 378 CCGG C^CGG
735 CCGG
HpaII 378 CCGG C^CGG
735 CCGG
MflI 170 AGATCC [GA]^GATC[CT]
374 AGATCC
MspI 378 CCGG C^CGG
735 CCGG
RsaI 418 GTAC GT^AC
759 GTAC
ScrFI 434 CCAGG CC^[ACGT]GG
734 CCCGG
StyI 288 CCAAGG C^C[AT][AT]GG
296 CCAAGG
Tsp45I 616 GTGAC ^GT[GC]AC
685 GTCAC
Tsp509I 94 AATT ^AATT
563 AATT
XhoII 170 AGATCC [GA]^GATC[CT]
374 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AlwI AlwNI BbvI BsaJI
BstF5I Cfr13I DdeI DpnII Fnu4HI
FokI HinfI ItaI MaeIII MboI
MboII MnlI NdeII NlaIII NlaIV
Sau3AI Sau96I SfaNI TfiI TseI