Restriction map for
pF1KB6368
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BstAPI BstBI
BstEII BstPI Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 478 GTATAC GT^[AC][GT]AC
AcsI 658 AAATTC [GA]^AATT[CT]
AlwI 262 GATCC GGATC(4/5)
ApoI 658 AAATTC [GA]^AATT[CT]
BalI 287 TGGCCA TGG^CCA
BfaI 790 CTAG C^TAG
BsgI 37 CTGCAC GTGCAG(16/14)
BsiEI 778 CGGCCG CG[GA][CT]^CG
BspMI 180 ACCTGC ACCTGC(4/8)
BsrBI 861 CCGCTC CCGCTC(-3/-3)
BssHII 798 GCGCGC G^CGCGC
BssSI 574 CTCGTG CACGAG(-5/-1)
Bst1107I 478 GTATAC GTA^TAC
BstYI 261 AGATCC [GA]^GATC[CT]
BstZ17I 478 GTATAC GTA^TAC
DpnII 262 GATC ^GATC
DraIII 688 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 73 CCACGG C^C[GA][CT]GG
EagI 778 CGGCCG C^GGCCG
EclXI 778 CGGCCG C^GGCCG
Eco52I 778 CGGCCG C^GGCCG
EcoT14I 755 CCAAGG C^C[AT][AT]GG
FseI 779 GGCCGGCC GGCCGG^CC
HphI 178 TCACC GGTGA(8/7)
MaeI 790 CTAG C^TAG
MboI 262 GATC ^GATC
MflI 261 AGATCC [GA]^GATC[CT]
MluNI 287 TGGCCA TGG^CCA
MscI 287 TGGCCA TGG^CCA
NaeI 780 GCCGGC GCC^GGC
NdeII 262 GATC ^GATC
NgoMIV 780 GCCGGC G^CCGGC
PflMI 876 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 702 GCTCTTC GCTCTTC(1/4)
Sau3AI 262 GATC ^GATC
SbfI 450 CCTGCAGG CCTGCA^GG
SgrAI 40 CACCGGCG C[GA]^CCGG[CT]G
SmlI 853 CTCAAG C^T[CT][GA]AG
Sse8387I 450 CCTGCAGG CCTGCA^GG
StyI 755 CCAAGG C^C[AT][AT]GG
TfiI 205 GAATC G^A[AT]TC
Tsp509I 659 AATT ^AATT
Van91I 876 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 566 CCATCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 261 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 631 CGCG CG^CG
799 CGCG
AvaII 318 GGACC G^G[AT]CC
356 GGACC
BanII 520 GAGCTC G[GA]GC[CT]^C
700 GAGCTC
BpmI 266 CTGGAG CTGGAG(16/14)
432 CTGGAG
BsmAI 667 GAGAC GTCTC(1/5)
613 GTCTC
BsrFI 41 ACCGGC [GA]^CCGG[CT]
780 GCCGGC
BsrI 387 ACTGG ACTGG(1/-1)
420 ACTGG
BstUI 631 CGCG CG^CG
799 CGCG
Cfr10I 41 ACCGGC [GA]^CCGG[CT]
780 GCCGGC
DdeI 97 CTGAG C^T[ACGT]AG
518 CTGAG
DraII 317 GGGACCT [GA]G^G[ACGT]CC[CT]
355 AGGACCC
EarI 703 CTCTTC CTCTTC(1/4)
622 GAAGAG
EcoO109I 317 GGGACCT [GA]G^G[ACGT]CC[CT]
355 AGGACCC
Ksp632I 703 CTCTTC CTCTTC(1/4)
622 GAAGAG
MslI 384 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
570 CATCCTCGTG
MvnI 631 CGCG CG^CG
799 CGCG
PleI 214 GACTC GAGTC(4/5)
442 GAGTC
PpuMI 317 GGGACCT [GA]G^G[AT]CC[CT]
355 AGGACCC
PstI 451 CTGCAG CTGCA^G
682 CTGCAG
PvuII 331 CAGCTG CAG^CTG
532 CAGCTG
SacI 520 GAGCTC GAGCT^C
700 GAGCTC
SfaNI 126 GCATC GCATC(5/9)
527 GCATC
SfcI 451 CTGCAG C^T[GA][CT]AG
682 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TaqI
TseI TspRI TthHB8I