Restriction map for
pF1KB6427
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI SspI SwaI Tru9I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AcsI 649 AAATTC [GA]^AATT[CT]
Alw44I 242 GTGCAC G^TGCAC
ApaLI 242 GTGCAC G^TGCAC
ApoI 649 AAATTC [GA]^AATT[CT]
AspI 228 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 297 CTCGAG C^[CT]CG[GA]G
AvrII 366 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BbsI 811 GAAGAC GAAGAC(2/6)
BbvCI 580 GCTGAGG CCTCAGC(-5/-2)
BcnI 566 CCGGG CC^[GC]GG
BfaI 367 CTAG C^TAG
BlnI 366 CCTAGG C^CTAGG
BlpI 30 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
BpuAI 811 GAAGAC GAAGAC(2/6)
BsaI 691 GGTCTC GGTCTC(1/5)
BsmBI 154 CGTCTC CGTCTC(1/5)
BsmFI 234 GTCCC GGGAC(10/14)
BsmI 897 GAATGC GAATGC(1/-1)
BsoBI 297 CTCGAG C^[CT]CG[GA]G
Bsp1407I 657 TGTACA T^GTACA
BsrFI 904 ACCGGC [GA]^CCGG[CT]
BsrGI 657 TGTACA T^GTACA
BssSI 793 CACGAG CACGAG(-5/-1)
CelII 30 GCTGAGC GC^T[ACGT]AGC
CfoI 456 GCGC GCG^C
Cfr10I 904 ACCGGC [GA]^CCGG[CT]
DraIII 616 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 424 CTCTTC CTCTTC(1/4)
EcoNI 306 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 19 GATATC GAT^ATC
HgaI 153 GCGTC GACGC(5/10)
HhaI 456 GCGC GCG^C
HinP1I 456 GCGC G^CGC
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
HindIII 278 AAGCTT A^AGCTT
HinfI 93 GAATC G^A[ACGT]TC
Ksp632I 424 CTCTTC CTCTTC(1/4)
MaeI 367 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MwoI 252 GCAAGCCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 566 CCGGG CC^[GC]GG
NcoI 164 CCATGG C^CATGG
NlaIII 165 CATG CATG^
PaeR7I 297 CTCGAG C^TCGAG
PflFI 228 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 138 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 309 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 862 CTGCAG CTGCA^G
SapI 423 GCTCTTC GCTCTTC(1/4)
SbfI 861 CCTGCAGG CCTGCA^GG
ScaI 593 AGTACT AGT^ACT
SexAI 617 ACCTGGT A^CC[AT]GGT
SfaNI 415 GATGC GCATC(5/9)
SgrAI 903 CACCGGCG C[GA]^CCGG[CT]G
SmlI 297 CTCGAG C^T[CT][GA]AG
Sse8387I 861 CCTGCAGG CCTGCA^GG
SspBI 657 TGTACA T^GTACA
StuI 572 AGGCCT AGG^CCT
TaqI 298 TCGA T^CGA
TfiI 93 GAATC G^A[AT]TC
TspRI 702 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 228 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 298 TCGA T^CGA
Van91I 138 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 902 CCACCGGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 297 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 435 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAACCCCTTC
BanII 33 GAGCTC G[GA]GC[CT]^C
478 GAGCTC
BpmI 379 CTGGAG CTGGAG(16/14)
604 CTGGAG
BsmAI 155 GTCTC GTCTC(1/5)
692 GTCTC
BstXI 775 CCAGATAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGCCGGATGG
DpnII 357 GATC ^GATC
409 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
837 TGGCCG
HphI 67 GGTGA GGTGA(8/7)
349 GGTGA
MboI 357 GATC ^GATC
409 GATC
MboII 811 GAAGA GAAGA(8/7)
425 TCTTC
MslI 61 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
219 CATCTGTGTG
NdeII 357 GATC ^GATC
409 GATC
SacI 33 GAGCTC GAGCT^C
478 GAGCTC
Sau3AI 357 GATC ^GATC
409 GATC
SfcI 738 CTATAG C^T[GA][CT]AG
862 CTGCAG
Tsp509I 650 AATT ^AATT
781 AATT
XmnI 435 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAACCCCTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeIII MnlI
MspA1I MspI MvaI NlaIV PpuMI
PvuII RsaI Sau96I ScrFI StyI
TseI Tsp45I