Restriction map for
pF1KB6442
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AccI AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AviII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MseI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 861 GACGTC GACGT^C
Acc65I 608 GGTACC G^GTACC
AlwI 536 GGATC GGATC(4/5)
Asp700I 69 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 608 GGTACC G^GTACC
AspHI 724 GAGCTC G[AT]GC[AT]^C
AspI 619 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 886 CCCGAG C^[CT]CG[GA]G
AvaII 258 GGACC G^G[AT]CC
AvrII 108 CCTAGG C^CTAGG
BanI 608 GGTACC G^G[CT][GA]CC
BcnI 423 CCGGG CC^[GC]GG
BglI 1024 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 239 AGATCT A^GATCT
BlnI 108 CCTAGG C^CTAGG
BlpI 762 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 762 GCTGAGC GC^T[ACGT]AGC
BsaI 940 GAGACC GGTCTC(1/5)
BsiHKAI 724 GAGCTC G[AT]GC[AT]^C
BsmAI 940 GAGAC GTCTC(1/5)
BsoBI 886 CCCGAG C^[CT]CG[GA]G
BspMI 443 GCAGGT ACCTGC(4/8)
BstEII 828 GGTGACC G^GT[ACGT]ACC
BstPI 828 GGTGACC G^GT[ACGT]ACC
BstYI 239 AGATCT [GA]^GATC[CT]
CelII 762 GCTGAGC GC^T[ACGT]AGC
DraII 1022 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1022 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 828 GGTGACC G^GT[ACGT]ACC
FauI 36 GCGGG CCCGC(4/6)
HaeII 3 GGCGCT [GA]GCGC^[CT]
KpnI 608 GGTACC GGTAC^C
MfeI 839 CAATTG C^AATTG
MflI 239 AGATCT [GA]^GATC[CT]
MslI 465 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 633 CAGCTG C[AC]G^C[GT]G
MunI 839 CAATTG C^AATTG
NciI 423 CCGGG CC^[GC]GG
NcoI 791 CCATGG C^CATGG
NspI 470 ACATGC [GA]CATG^[CT]
PflFI 619 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1027 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 633 CAGCTG CAG^CTG
SacI 724 GAGCTC GAGCT^C
ScaI 416 AGTACT AGT^ACT
SmlI 400 CTCAAG C^T[CT][GA]AG
StuI 950 AGGCCT AGG^CCT
TspRI 532 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 619 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1027 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 239 AGATCT [GA]^GATC[CT]
XmnI 69 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 633 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1028 CAGAGGCTG
BbsI 459 GAAGAC GAAGAC(2/6)
217 GTCTTC
BfaI 109 CTAG C^TAG
647 CTAG
BpuAI 459 GAAGAC GAAGAC(2/6)
217 GTCTTC
DsaI 655 CCACGG C^C[GA][CT]GG
791 CCATGG
HapII 423 CCGG C^CGG
937 CCGG
HgaI 250 GACGC GACGC(5/10)
623 GCGTC
HpaII 423 CCGG C^CGG
937 CCGG
HphI 828 GGTGA GGTGA(8/7)
680 TCACC
MaeI 109 CTAG C^TAG
647 CTAG
MaeII 596 ACGT A^CGT
862 ACGT
MspI 423 CCGG C^CGG
937 CCGG
PstI 475 CTGCAG CTGCA^G
747 CTGCAG
SapI 927 GAAGAGC GCTCTTC(1/4)
819 GCTCTTC
SexAI 611 ACCTGGT A^CC[AT]GGT
989 ACCAGGT
SfaNI 92 GCATC GCATC(5/9)
665 GCATC
Tsp45I 802 GTCAC ^GT[GC]AC
829 GTGAC
Tsp509I 160 AATT ^AATT
840 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AfaI AluI BanII
BbvI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI Hin1I HinP1I HinfI
ItaI Ksp632I MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV RcaI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TfiI
TseI TthHB8I