Restriction map for
pF1KB6448
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MflI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 128 CGCG CG^CG
AccIII 660 TCCGGA T^CCGGA
AcsI 370 AAATTT [GA]^AATT[CT]
AlwI 663 GGATC GGATC(4/5)
ApaI 1009 GGGCCC GGGCC^C
ApoI 370 AAATTT [GA]^AATT[CT]
AseI 508 ATTAAT AT^TAAT
AsnI 508 ATTAAT AT^TAAT
AviII 95 TGCGCA TGC^GCA
BciVI 210 GTATCC GTATCC(6/5)
BclI 1028 TGATCA T^GATCA
BfaI 116 CTAG C^TAG
BsaBI 864 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 660 TCCGGA [AT]^CCGG[AT]
BseAI 660 TCCGGA T^CCGGA
BsmAI 263 GAGAC GTCTC(1/5)
BsmFI 227 GGGAC GGGAC(10/14)
Bsp1407I 275 TGTACA T^GTACA
BspEI 660 TCCGGA T^CCGGA
BspHI 375 TCATGA T^CATGA
BsrDI 257 GCAATG GCAATG(2/0)
BsrGI 275 TGTACA T^GTACA
BstUI 128 CGCG CG^CG
BstXI 820 CCAATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 283 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 96 GCGC GCG^C
EarI 421 GAAGAG CTCTTC(1/4)
EcoT14I 986 CCTTGG C^C[AT][AT]GG
EcoT22I 645 ATGCAT ATGCA^T
FauI 73 CCCGC CCCGC(4/6)
FbaI 1028 TGATCA T^GATCA
FspI 95 TGCGCA TGC^GCA
HgaI 129 GCGTC GACGC(5/10)
HhaI 96 GCGC GCG^C
HinP1I 96 GCGC G^CGC
Ksp632I 421 GAAGAG CTCTTC(1/4)
MaeI 116 CTAG C^TAG
MamI 864 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 660 TCCGGA T^CCGGA
MspA1I 11 CAGCGG C[AC]G^C[GT]G
MvnI 128 CGCG CG^CG
NsiI 645 ATGCAT ATGCA^T
PflMI 765 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 227 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 508 ATTAAT AT^TAAT
RcaI 375 TCATGA T^CATGA
SspBI 275 TGTACA T^GTACA
SspI 250 AATATT AAT^ATT
StuI 983 AGGCCT AGG^CCT
StyI 986 CCTTGG C^C[AT][AT]GG
TaqI 671 TCGA T^CGA
TfiI 85 GAATC G^A[AT]TC
TthHB8I 671 TCGA T^CGA
Van91I 765 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 47 CCCGGG C^[CT]CG[GA]G
149 CCCGGG
AvaII 228 GGACC G^G[AT]CC
350 GGTCC
BalI 323 TGGCCA TGG^CCA
947 TGGCCA
BpmI 38 CTGGAG CTGGAG(16/14)
59 CTGGAG
BsoBI 47 CCCGGG C^[CT]CG[GA]G
149 CCCGGG
BstF5I 816 CATCC GGATG(2/0)
81 GGATG
DpnII 664 GATC ^GATC
1029 GATC
EaeI 323 TGGCCA [CT]^GGCC[GA]
947 TGGCCA
FokI 816 CATCC GGATG(9/13)
81 GGATG
HphI 802 GGTGA GGTGA(8/7)
699 TCACC
MboI 664 GATC ^GATC
1029 GATC
MluNI 323 TGGCCA TGG^CCA
947 TGGCCA
MscI 323 TGGCCA TGG^CCA
947 TGGCCA
NdeII 664 GATC ^GATC
1029 GATC
PleI 135 GAGTC GAGTC(4/5)
844 GAGTC
PstI 394 CTGCAG CTGCA^G
436 CTGCAG
Sau3AI 664 GATC ^GATC
1029 GATC
SfcI 394 CTGCAG C^T[GA][CT]AG
436 CTGCAG
SmaI 47 CCCGGG CCC^GGG
149 CCCGGG
Tsp45I 583 GTGAC ^GT[GC]AC
707 GTGAC
XcmI 765 CCAAAATGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
820 CCAATGTACTGGTGG
XmaCI 47 CCCGGG C^CCGGG
149 CCCGGG
XmaI 47 CCCGGG C^CCGGG
149 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstNI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeIII MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI Tru9I TseI Tsp509I
TspRI