Restriction map for
pF1KB6475
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoT14I
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MluI MluNI MroI MscI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 64 GGCGCC G[GA]^CG[CT]C
AflIII 297 ACATGT A^C[GA][CT]GT
Alw44I 246 GTGCAC G^TGCAC
AlwI 600 GGATC GGATC(4/5)
ApaLI 246 GTGCAC G^TGCAC
AvaII 903 GGACC G^G[AT]CC
BbeI 64 GGCGCC GGCGC^C
BbsI 721 GAAGAC GAAGAC(2/6)
BbvCI 192 GCTGAGG CCTCAGC(-5/-2)
BclI 675 TGATCA T^GATCA
BpuAI 721 GAAGAC GAAGAC(2/6)
BsaBI 670 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 64 GGCGCC G[GA]^CG[CT]C
BsaI 205 GAGACC GGTCTC(1/5)
BseRI 151 GAGGAG GAGGAG(10/8)
BsgI 139 GTGCAG GTGCAG(16/14)
BsmI 40 GAATGC GAATGC(1/-1)
BspDI 805 ATCGAT AT^CGAT
BspLU11I 297 ACATGT A^CATGT
BspMI 938 ACCTGC ACCTGC(4/8)
BsrBI 163 CCGCTC CCGCTC(-3/-3)
BsrFI 145 ACCGGC [GA]^CCGG[CT]
BstEII 456 GGTGACC G^GT[ACGT]ACC
BstF5I 174 CATCC GGATG(2/0)
BstPI 456 GGTGACC G^GT[ACGT]ACC
BstYI 600 GGATCT [GA]^GATC[CT]
Cfr10I 145 ACCGGC [GA]^CCGG[CT]
ClaI 805 ATCGAT AT^CGAT
DraII 902 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 543 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 596 CCGTGG C^C[GA][CT]GG
EcoO109I 902 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 456 GGTGACC G^GT[ACGT]ACC
EcoRV 802 GATATC GAT^ATC
FbaI 675 TGATCA T^GATCA
FokI 174 CATCC GGATG(9/13)
HaeII 64 GGCGCC [GA]GCGC^[CT]
HapII 146 CCGG C^CGG
Hin1I 64 GGCGCC G[GA]^CG[CT]C
HpaII 146 CCGG C^CGG
KasI 64 GGCGCC G^GCGCC
MamI 670 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 782 CAATTG C^AATTG
MflI 600 GGATCT [GA]^GATC[CT]
MseI 848 TTAA T^TAA
MspA1I 465 CAGCGG C[AC]G^C[GT]G
MspI 146 CCGG C^CGG
MunI 782 CAATTG C^AATTG
NarI 64 GGCGCC GG^CGCC
NruI 429 TCGCGA TCG^CGA
PleI 707 GACTC GAGTC(4/5)
PpuMI 902 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 64 GGCGCC GGC^GCC
SphI 158 GCATGC GCATG^C
TfiI 931 GATTC G^A[AT]TC
Tru9I 848 TTAA T^TAA
Tsp509I 783 AATT ^AATT
XcmI 173 CCATCCGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 600 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 36 GTAC GT^AC
552 GTAC
AlwNI 338 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
901 CAGGACCTG
Asp700I 75 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
631 GAAATGTTTC
BanI 64 GGCGCC G^G[CT][GA]CC
197 GGCACC
BbvI 464 GCAGC GCAGC(8/12)
617 GCTGC
BsmAI 205 GAGAC GTCTC(1/5)
885 GTCTC
BsrI 725 ACTGG ACTGG(1/-1)
576 CCAGT
BstXI 317 CCACTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
590 CCACCCCCGTGG
Cfr13I 87 GGCCC G^G[ACGT]CC
903 GGACC
DdeI 193 CTGAG C^T[ACGT]AG
531 CTCAG
HinfI 707 GACTC G^A[ACGT]TC
931 GATTC
HphI 456 GGTGA GGTGA(8/7)
888 TCACC
MaeII 224 ACGT A^CGT
698 ACGT
MslI 450 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
591 CACCCCCGTG
NlaIII 159 CATG CATG^
298 CATG
NlaIV 64 GGCGCC GG[ACGT]^[ACGT]CC
197 GGCACC
NspI 158 GCATGC [GA]CATG^[CT]
297 ACATGT
PflMI 337 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGGACCTGG
RsaI 36 GTAC GT^AC
552 GTAC
Sau96I 87 GGCCC G^G[ACGT]CC
903 GGACC
SfaNI 426 GCATC GCATC(5/9)
503 GCATC
SfcI 507 CTATAG C^T[GA][CT]AG
873 CTACAG
TseI 464 GCAGC G^C[AT]GC
617 GCTGC
Van91I 337 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGGACCTGG
XmnI 75 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
631 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AspHI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI DpnII EcoRII FauI
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeI MaeIII MboI MboII MnlI
MvaI MvnI MwoI NdeII Sau3AI
ScrFI TaqI Tsp45I TspRI TthHB8I