Restriction map for
pF1KB6478
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tsp45I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AflIII 404 ACATGT A^C[GA][CT]GT
AspHI 391 GAGCTC G[AT]GC[AT]^C
BbsI 129 GTCTTC GAAGAC(2/6)
BbvCI 540 GCTGAGG CCTCAGC(-5/-2)
BcgI 738 CGACCGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 556 CTAG C^TAG
BglII 65 AGATCT A^GATCT
BpuAI 129 GTCTTC GAAGAC(2/6)
BsiHKAI 391 GAGCTC G[AT]GC[AT]^C
BsiWI 32 CGTACG C^GTACG
BsmAI 449 GAGAC GTCTC(1/5)
BspLU11I 404 ACATGT A^CATGT
BssHII 263 GCGCGC G^CGCGC
BstXI 629 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
HgaI 435 GACGC GACGC(5/10)
MaeI 556 CTAG C^TAG
NspI 404 ACATGT [GA]CATG^[CT]
PflMI 336 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 298 CAGCTG CAG^CTG
SacI 391 GAGCTC GAGCT^C
SbfI 527 CCTGCAGG CCTGCA^GG
Sse8387I 527 CCTGCAGG CCTGCA^GG
TfiI 904 GAATC G^A[AT]TC
Tsp509I 10 AATT ^AATT
TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 336 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AspI 451 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACGAGGTC
BanI 154 GGTGCC G^G[CT][GA]CC
463 GGCACC
BglI 598 GCCGATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
692 GCCGGGGAGGC
BsgI 495 CTGCAC GTGCAG(16/14)
295 GTGCAG
BsiEI 579 CGACCG CG[GA][CT]^CG
738 CGACCG
BspHI 782 TCATGA T^CATGA
794 TCATGA
BspMI 671 ACCTGC ACCTGC(4/8)
719 ACCTGC
BsrBI 91 GAGCGG CCGCTC(-3/-3)
1000 GAGCGG
DraII 195 GGGTCCC [GA]G^G[ACGT]CC[CT]
584 GGGACCT
EcoO109I 195 GGGTCCC [GA]G^G[ACGT]CC[CT]
584 GGGACCT
HincII 715 GTCAAC GT[CT]^[GA]AC
772 GTTGAC
HindII 715 GTCAAC GT[CT]^[GA]AC
772 GTTGAC
HphI 6 GGTGA GGTGA(8/7)
1041 GGTGA
PflFI 451 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACGAGGTC
PleI 568 GACTC GAGTC(4/5)
206 GAGTC
PpuMI 195 GGGTCCC [GA]G^G[AT]CC[CT]
584 GGGACCT
RcaI 782 TCATGA T^CATGA
794 TCATGA
TaqI 71 TCGA T^CGA
245 TCGA
Tth111I 451 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
775 GACGAGGTC
TthHB8I 71 TCGA T^CGA
245 TCGA
XcmI 374 CCAGGGACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
878 CCACCCCCAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI XhoII