Restriction map for
pF1KB6483
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaWI BseAI BsiEI BsmBI
BsmI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 139 GACGCC G[GA]^CG[CT]C
Alw44I 100 GTGCAC G^TGCAC
AlwNI 829 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 50 GGGCCC GGGCC^C
ApaLI 100 GTGCAC G^TGCAC
Asp700I 448 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 100 GTGCAC G[AT]GC[AT]^C
AviII 413 TGCGCA TGC^GCA
BanII 50 GGGCCC G[GA]GC[CT]^C
BglI 80 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 888 GCTGAGC GC^T[ACGT]AGC
BpmI 625 CTGGAG CTGGAG(16/14)
Bpu1102I 888 GCTGAGC GC^T[ACGT]AGC
BsaAI 369 CACGTA [CT]AC^GT[GA]
BsaHI 139 GACGCC G[GA]^CG[CT]C
BsaI 475 GAGACC GGTCTC(1/5)
BsiHKAI 100 GTGCAC G[AT]GC[AT]^C
BsiWI 371 CGTACG C^GTACG
BsmAI 475 GAGAC GTCTC(1/5)
BspHI 456 TCATGA T^CATGA
BspLU11I 920 ACATGT A^CATGT
BspMI 851 ACCTGC ACCTGC(4/8)
BsrBI 505 GAGCGG CCGCTC(-3/-3)
BsrFI 714 GCCGGC [GA]^CCGG[CT]
BssSI 859 CACGAG CACGAG(-5/-1)
CelII 888 GCTGAGC GC^T[ACGT]AGC
Cfr10I 714 GCCGGC [GA]^CCGG[CT]
CpoI 380 CGGACCG CG^G[AT]CCG
DraIII 802 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 144 CCACGG C^C[GA][CT]GG
EcoRI 199 GAATTC G^AATTC
EcoT14I 194 CCAAGG C^C[AT][AT]GG
FspI 413 TGCGCA TGC^GCA
Hin1I 139 GACGCC G[GA]^CG[CT]C
MboII 448 GAAGA GAAGA(8/7)
MspA1I 331 CAGCGG C[AC]G^C[GT]G
NaeI 714 GCCGGC GCC^GGC
NgoMIV 714 GCCGGC G^CCGGC
PaeR7I 551 CTCGAG C^TCGAG
PflMI 828 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 456 TCATGA T^CATGA
RsrII 380 CGGACCG CG^G[AT]CCG
SfiI 79 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 551 CTCGAG C^T[CT][GA]AG
StyI 194 CCAAGG C^C[AT][AT]GG
TspRI 802 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 828 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 551 CTCGAG C^TCGAG
XmnI 448 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 199 GAATTC [GA]^AATT[CT]
451 GAATTT
AflIII 368 ACACGT A^C[GA][CT]GT
920 ACATGT
ApoI 199 GAATTC [GA]^AATT[CT]
451 GAATTT
BanI 776 GGCACC G^G[CT][GA]CC
968 GGCACC
BsrI 803 ACTGG ACTGG(1/-1)
156 CCAGT
BstYI 681 GGATCT [GA]^GATC[CT]
759 GGATCT
FauI 268 GCGGG CCCGC(4/6)
600 GCGGG
HinfI 346 GACTC G^A[ACGT]TC
538 GACTC
MaeII 370 ACGT A^CGT
461 ACGT
MflI 681 GGATCT [GA]^GATC[CT]
759 GGATCT
NspI 596 ACATGC [GA]CATG^[CT]
920 ACATGT
PleI 346 GACTC GAGTC(4/5)
538 GACTC
PpuMI 629 AGGACCT [GA]G^G[AT]CC[CT]
956 AGGACCT
SfaNI 469 GATGC GCATC(5/9)
489 GATGC
XhoII 681 GGATCT [GA]^GATC[CT]
759 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BbvI BcnI
BmyI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MboI MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
TseI Tsp509I TthHB8I