Restriction map for
pF1KB6504
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
Cfr10I ClaI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1005 GGTACC G^GTACC
Asp700I 513 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1005 GGTACC G^GTACC
BalI 455 TGGCCA TGG^CCA
BbrPI 391 CACGTG CAC^GTG
BbsI 973 GAAGAC GAAGAC(2/6)
BbvCI 873 GCTGAGG CCTCAGC(-5/-2)
BlpI 612 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 612 GCTGAGC GC^T[ACGT]AGC
BpuAI 973 GAAGAC GAAGAC(2/6)
BsaAI 391 CACGTG [CT]AC^GT[GA]
BsgI 895 CTGCAC GTGCAG(16/14)
BsmBI 274 CGTCTC CGTCTC(1/5)
BsmI 1109 GCATTC GAATGC(1/-1)
BsrBI 156 GAGCGG CCGCTC(-3/-3)
BsrDI 446 GCAATG GCAATG(2/0)
BstEII 642 GGTGACC G^GT[ACGT]ACC
BstPI 642 GGTGACC G^GT[ACGT]ACC
CelII 612 GCTGAGC GC^T[ACGT]AGC
CpoI 854 CGGTCCG CG^G[AT]CCG
DrdI 1063 GACACCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 642 GGTGACC G^GT[ACGT]ACC
KpnI 1005 GGTACC GGTAC^C
MluNI 455 TGGCCA TGG^CCA
MscI 455 TGGCCA TGG^CCA
PflMI 732 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 391 CACGTG CAC^GTG
PmlI 391 CACGTG CAC^GTG
PstI 913 CTGCAG CTGCA^G
RsrII 854 CGGTCCG CG^G[AT]CCG
SacI 787 GAGCTC GAGCT^C
TfiI 556 GATTC G^A[AT]TC
Van91I 732 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 268 CCACAGCGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 513 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 16 CGCG CG^CG
92 CGCG
BseRI 178 GAGGAG GAGGAG(10/8)
876 GAGGAG
BsmAI 275 GTCTC GTCTC(1/5)
918 GTCTC
BspMI 908 ACCTGC ACCTGC(4/8)
382 GCAGGT
BstUI 16 CGCG CG^CG
92 CGCG
DraII 206 GGGTCCC [GA]G^G[ACGT]CC[CT]
693 GGGACCC
EaeI 125 TGGCCG [CT]^GGCC[GA]
455 TGGCCA
EcoO109I 206 GGGTCCC [GA]G^G[ACGT]CC[CT]
693 GGGACCC
EcoT14I 469 CCAAGG C^C[AT][AT]GG
698 CCAAGG
HgaI 273 GCGTC GACGC(5/10)
891 GCGTC
KspI 15 CCGCGG CCGC^GG
91 CCGCGG
MaeII 45 ACGT A^CGT
392 ACGT
MaeIII 482 GTCAC ^GT[ACGT]AC
643 GTGAC
MvnI 16 CGCG CG^CG
92 CGCG
NlaIII 54 CATG CATG^
738 CATG
PpuMI 206 GGGTCCC [GA]G^G[AT]CC[CT]
693 GGGACCC
SacII 15 CCGCGG CCGC^GG
91 CCGCGG
SapI 516 GCTCTTC GCTCTTC(1/4)
657 GCTCTTC
SfcI 834 CTACAG C^T[GA][CT]AG
913 CTGCAG
StyI 469 CCAAGG C^C[AT][AT]GG
698 CCAAGG
TaqI 511 TCGA T^CGA
532 TCGA
Tsp45I 482 GTCAC ^GT[GC]AC
643 GTGAC
TspRI 434 CAGTG CA[GC]TG[ACGT][ACGT]^
461 CACTG
TthHB8I 511 TCGA T^CGA
532 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SmaI TseI
XhoII XmaCI XmaI