Restriction map for
pF1KB6518
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SalI
SbfI SexAI SfiI SfoI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 448 GTCTAC GT^[AC][GT]AC
AcyI 613 GACGCC G[GA]^CG[CT]C
ApaI 89 GGGCCC GGGCC^C
AspHI 642 GAGCTC G[AT]GC[AT]^C
AspI 949 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 21 CCCGGG C^[CT]CG[GA]G
BanI 939 GGTGCC G^G[CT][GA]CC
BfaI 325 CTAG C^TAG
BsaAI 547 TACGTG [CT]AC^GT[GA]
BsaHI 613 GACGCC G[GA]^CG[CT]C
BsaWI 152 ACCGGA [AT]^CCGG[AT]
BsiHKAI 642 GAGCTC G[AT]GC[AT]^C
BsmI 383 GCATTC GAATGC(1/-1)
BsoBI 21 CCCGGG C^[CT]CG[GA]G
BsrFI 1045 GCCGGC [GA]^CCGG[CT]
BstAPI 1113 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 287 CATCC GGATG(2/0)
BstXI 101 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1153 GCGC GCG^C
Cfr10I 1045 GCCGGC [GA]^CCGG[CT]
CpoI 1130 CGGACCG CG^G[AT]CCG
DdeI 114 CTCAG C^T[ACGT]AG
EcoNI 846 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 287 CATCC GGATG(9/13)
HhaI 1153 GCGC GCG^C
Hin1I 613 GACGCC G[GA]^CG[CT]C
HinP1I 1153 GCGC G^CGC
HinfI 52 GACTC G^A[ACGT]TC
HphI 767 TCACC GGTGA(8/7)
MaeI 325 CTAG C^TAG
NaeI 1045 GCCGGC GCC^GGC
NgoMIV 1045 GCCGGC G^CCGGC
NotI 1039 GCGGCCGC GC^GGCCGC
NspI 1076 ACATGC [GA]CATG^[CT]
PflFI 949 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 369 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 52 GACTC GAGTC(4/5)
PshAI 52 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1130 CGGACCG CG^G[AT]CCG
SacI 642 GAGCTC GAGCT^C
ScaI 338 AGTACT AGT^ACT
SgrAI 1044 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 21 CCCGGG CCC^GGG
Tsp45I 947 GTGAC ^GT[GC]AC
Tth111I 949 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 369 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 698 CGCG CG^CG
1091 CGCG
AlwI 826 GATCC GGATC(4/5)
414 GGATC
BglI 1030 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1045 GCCGGCGGGGC
BstUI 698 CGCG CG^CG
1091 CGCG
BstYI 414 GGATCT [GA]^GATC[CT]
825 AGATCC
DpnII 415 GATC ^GATC
826 GATC
EagI 755 CGGCCG C^GGCCG
1040 CGGCCG
EarI 156 GAAGAG CTCTTC(1/4)
183 GAAGAG
EclXI 755 CGGCCG C^GGCCG
1040 CGGCCG
Eco52I 755 CGGCCG C^GGCCG
1040 CGGCCG
EcoT14I 410 CCTTGG C^C[AT][AT]GG
485 CCAAGG
HgaI 501 GACGC GACGC(5/10)
613 GACGC
HindIII 715 AAGCTT A^AGCTT
923 AAGCTT
Ksp632I 156 GAAGAG CTCTTC(1/4)
183 GAAGAG
KspI 697 CCGCGG CCGC^GG
1090 CCGCGG
MaeIII 239 GTTAC ^GT[ACGT]AC
947 GTGAC
MboI 415 GATC ^GATC
826 GATC
MflI 414 GGATCT [GA]^GATC[CT]
825 AGATCC
MslI 99 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
576 CATGGCGGTG
MvnI 698 CGCG CG^CG
1091 CGCG
NdeII 415 GATC ^GATC
826 GATC
PpuMI 539 AGGTCCC [GA]G^G[AT]CC[CT]
632 GGGTCCC
PvuII 132 CAGCTG CAG^CTG
370 CAGCTG
SacII 697 CCGCGG CCGC^GG
1090 CCGCGG
SapI 156 GAAGAGC GCTCTTC(1/4)
183 GAAGAGC
Sau3AI 415 GATC ^GATC
826 GATC
SfaNI 62 GCATC GCATC(5/9)
492 GCATC
StuI 117 AGGCCT AGG^CCT
671 AGGCCT
StyI 410 CCTTGG C^C[AT][AT]GG
485 CCAAGG
TaqI 593 TCGA T^CGA
935 TCGA
TthHB8I 593 TCGA T^CGA
935 TCGA
XhoII 414 GGATCT [GA]^GATC[CT]
825 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstNI Cac8I Cfr13I DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HpaII ItaI MaeII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PstI RsaI Sau96I ScrFI
SfcI TseI TspRI XcmI