Restriction map for
pF1KB6396
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BfrI
BglII BlnI BpmI BsaAI BsaHI
BsaI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MflI
MluI MluNI MscI MslI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TseI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 422 TCCGGA T^CCGGA
Alw44I 797 GTGCAC G^TGCAC
AlwI 84 GGATC GGATC(4/5)
ApaLI 797 GTGCAC G^TGCAC
BanI 305 GGTGCC G^G[CT][GA]CC
BanII 324 GAGCTC G[GA]GC[CT]^C
BbsI 844 GAAGAC GAAGAC(2/6)
BcnI 285 CCGGG CC^[GC]GG
BfaI 398 CTAG C^TAG
BlpI 321 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 321 GCTGAGC GC^T[ACGT]AGC
BpuAI 844 GAAGAC GAAGAC(2/6)
BsaBI 234 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 422 TCCGGA [AT]^CCGG[AT]
BseAI 422 TCCGGA T^CCGGA
BspEI 422 TCCGGA T^CCGGA
BspMI 740 ACCTGC ACCTGC(4/8)
BsrDI 431 CATTGC GCAATG(2/0)
CelII 321 GCTGAGC GC^T[ACGT]AGC
CfoI 357 GCGC GCG^C
DpnII 85 GATC ^GATC
DsaI 699 CCATGG C^C[GA][CT]GG
EcoT14I 699 CCATGG C^C[AT][AT]GG
EcoT22I 347 ATGCAT ATGCA^T
Fnu4HI 525 GCCGC GC^[ACGT]GC
HaeII 356 AGCGCC [GA]GCGC^[CT]
HhaI 357 GCGC GCG^C
HinP1I 357 GCGC G^CGC
ItaI 525 GCCGC GC^[ACGT]GC
MaeI 398 CTAG C^TAG
MaeII 466 ACGT A^CGT
MaeIII 580 GTGAC ^GT[ACGT]AC
MamI 234 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 85 GATC ^GATC
MroI 422 TCCGGA T^CCGGA
NciI 285 CCGGG CC^[GC]GG
NcoI 699 CCATGG C^CATGG
NdeII 85 GATC ^GATC
NsiI 347 ATGCAT ATGCA^T
PflMI 336 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 324 GAGCTC GAGCT^C
SapI 505 GCTCTTC GCTCTTC(1/4)
Sau3AI 85 GATC ^GATC
SfaNI 346 GATGC GCATC(5/9)
SmlI 556 CTTGAG C^T[CT][GA]AG
StuI 671 AGGCCT AGG^CCT
StyI 699 CCATGG C^C[AT][AT]GG
TaqI 73 TCGA T^CGA
Tsp45I 580 GTGAC ^GT[GC]AC
TthHB8I 73 TCGA T^CGA
Van91I 336 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 320 AGCT AG^CT
325 AGCT
AvaII 56 GGACC G^G[AT]CC
696 GGTCC
BglI 592 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
610 GCCTCCTGGGC
BsmAI 484 GAGAC GTCTC(1/5)
710 GTCTC
BsmFI 287 GGGAC GGGAC(10/14)
468 GTCCC
BstF5I 490 CATCC GGATG(2/0)
345 GGATG
FauI 834 CCCGC CCCGC(4/6)
297 GCGGG
FokI 490 CATCC GGATG(9/13)
345 GGATG
HapII 285 CCGG C^CGG
423 CCGG
HinfI 279 GATTC G^A[ACGT]TC
658 GAATC
HpaII 285 CCGG C^CGG
423 CCGG
MfeI 113 CAATTG C^AATTG
313 CAATTG
MspI 285 CCGG C^CGG
423 CCGG
MunI 113 CAATTG C^AATTG
313 CAATTG
NlaIII 700 CATG CATG^
751 CATG
PstI 716 CTGCAG CTGCA^G
742 CTGCAG
SfcI 716 CTGCAG C^T[GA][CT]AG
742 CTGCAG
TfiI 279 GATTC G^A[AT]TC
658 GAATC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI ApoI AspHI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
EarI EcoRII HaeIII HphI Ksp632I
MboII MnlI MseI MvaI MwoI
NlaIV Sau96I ScrFI Tru9I Tsp509I
TspRI