Restriction map for
pF1KB6403
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BmyI BpuAI
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MamI MluI
MluNI MscI MspA1I MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 872 TCCGGA T^CCGGA
AcsI 502 AAATTT [GA]^AATT[CT]
AfaI 453 GTAC GT^AC
Aor51HI 895 AGCGCT AGC^GCT
ApoI 502 AAATTT [GA]^AATT[CT]
AvaII 751 GGACC G^G[AT]CC
BamHI 342 GGATCC G^GATCC
BciVI 607 GTATCC GTATCC(6/5)
BglII 540 AGATCT A^GATCT
BlpI 817 GCTCAGC GC^T[ACGT]AGC
BpmI 761 CTGGAG CTGGAG(16/14)
Bpu1102I 817 GCTCAGC GC^T[ACGT]AGC
BsaJI 74 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 872 TCCGGA [AT]^CCGG[AT]
BseAI 872 TCCGGA T^CCGGA
BsiYI 754 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 754 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 118 GAGAC GTCTC(1/5)
BsmI 906 GCATTC GAATGC(1/-1)
BspEI 872 TCCGGA T^CCGGA
BspMI 753 ACCTGC ACCTGC(4/8)
BssKI 18 CCTGG ^CC[ACGT]GG
BstNI 18 CCTGG CC^[AT]GG
CelII 817 GCTCAGC GC^T[ACGT]AGC
CfoI 896 GCGC GCG^C
EarI 901 GAAGAG CTCTTC(1/4)
Eco47III 895 AGCGCT AGC^GCT
EcoRII 18 CCTGG ^CC[AT]GG
FauI 802 GCGGG CCCGC(4/6)
HaeII 895 AGCGCT [GA]GCGC^[CT]
HapII 873 CCGG C^CGG
HhaI 896 GCGC GCG^C
HinP1I 896 GCGC G^CGC
HpaII 873 CCGG C^CGG
Ksp632I 901 GAAGAG CTCTTC(1/4)
MaeII 209 ACGT A^CGT
MfeI 440 CAATTG C^AATTG
MroI 872 TCCGGA T^CCGGA
MslI 672 CATTAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 873 CCGG C^CGG
MunI 440 CAATTG C^AATTG
MvaI 18 CCTGG CC^[AT]GG
NheI 646 GCTAGC G^CTAGC
PpuMI 750 GGGACCT [GA]G^G[AT]CC[CT]
PstI 308 CTGCAG CTGCA^G
RsaI 453 GTAC GT^AC
SapI 901 GAAGAGC GCTCTTC(1/4)
ScrFI 18 CCTGG CC^[ACGT]GG
SexAI 17 ACCTGGT A^CC[AT]GGT
SfaNI 409 GATGC GCATC(5/9)
SmlI 704 CTCAAG C^T[CT][GA]AG
StuI 461 AGGCCT AGG^CCT
Tsp45I 398 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 682 GCGG CCGC(-3/-1)
802 GCGG
AlwI 343 GATCC GGATC(4/5)
342 GGATC
AlwNI 230 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
620 CAGGAACTG
BfaI 154 CTAG C^TAG
647 CTAG
BsmFI 750 GGGAC GGGAC(10/14)
804 GGGAC
BstYI 342 GGATCC [GA]^GATC[CT]
540 AGATCT
Cac8I 480 GCTGGC GC[ACGT]^[ACGT]GC
646 GCTAGC
DraII 750 GGGACCT [GA]G^G[ACGT]CC[CT]
770 GGGGCCT
EcoO109I 750 GGGACCT [GA]G^G[ACGT]CC[CT]
770 GGGGCCT
HinfI 445 GATTC G^A[ACGT]TC
559 GATTC
MaeI 154 CTAG C^TAG
647 CTAG
MflI 342 GGATCC [GA]^GATC[CT]
540 AGATCT
MwoI 484 GCCCTCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
822 GCAGATACAGC
NlaIII 285 CATG CATG^
642 CATG
SfcI 308 CTGCAG C^T[GA][CT]AG
617 CTACAG
TfiI 445 GATTC G^A[AT]TC
559 GATTC
XhoII 342 GGATCC [GA]^GATC[CT]
540 AGATCT
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BbvI BsrI BstF5I Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
ItaI MaeIII MboI MboII MnlI
MseI NdeII NlaIV Sau3AI Sau96I
Tru9I TseI Tsp509I TspRI