Restriction map for
pF1KB6418
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 319 GTAGAC GT^[AC][GT]AC
AccII 39 CGCG CG^CG
AcyI 703 GGCGCC G[GA]^CG[CT]C
AfaI 108 GTAC GT^AC
AlwNI 175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 466 CTCGAG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BamHI 932 GGATCC G^GATCC
BbeI 703 GGCGCC GGCGC^C
BbsI 628 GAAGAC GAAGAC(2/6)
BciVI 307 GTATCC GTATCC(6/5)
BcnI 298 CCGGG CC^[GC]GG
BglI 826 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 964 CTGGAG CTGGAG(16/14)
BpuAI 628 GAAGAC GAAGAC(2/6)
BsaHI 703 GGCGCC G[GA]^CG[CT]C
BsmFI 138 GTCCC GGGAC(10/14)
BsoBI 466 CTCGAG C^[CT]CG[GA]G
BsrBI 967 GAGCGG CCGCTC(-3/-3)
BsrFI 742 GCCGGC [GA]^CCGG[CT]
BstAPI 482 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 39 CGCG CG^CG
BstYI 932 GGATCC [GA]^GATC[CT]
Cfr10I 742 GCCGGC [GA]^CCGG[CT]
DdeI 822 CTCAG C^T[ACGT]AG
DsaI 68 CCGTGG C^C[GA][CT]GG
EarI 505 CTCTTC CTCTTC(1/4)
EcoT14I 827 CCAAGG C^C[AT][AT]GG
HaeII 703 GGCGCC [GA]GCGC^[CT]
Hin1I 703 GGCGCC G[GA]^CG[CT]C
KasI 703 GGCGCC G^GCGCC
Ksp632I 505 CTCTTC CTCTTC(1/4)
MaeII 499 ACGT A^CGT
MflI 932 GGATCC [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MseI 239 TTAA T^TAA
MspA1I 947 CCGCTG C[AC]G^C[GT]G
MvnI 39 CGCG CG^CG
NaeI 742 GCCGGC GCC^GGC
NarI 703 GGCGCC GG^CGCC
NciI 298 CCGGG CC^[GC]GG
NgoMIV 742 GCCGGC G^CCGGC
NspI 588 GCATGT [GA]CATG^[CT]
PaeR7I 466 CTCGAG C^TCGAG
PleI 34 GAGTC GAGTC(4/5)
PstI 790 CTGCAG CTGCA^G
RsaI 108 GTAC GT^AC
SfcI 790 CTGCAG C^T[GA][CT]AG
SfoI 703 GGCGCC GGC^GCC
StyI 827 CCAAGG C^C[AT][AT]GG
TfiI 294 GATTC G^A[AT]TC
Tru9I 239 TTAA T^TAA
XhoI 466 CTCGAG C^TCGAG
XhoII 932 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 413 GAATTC [GA]^AATT[CT]
446 GAATTC
ApoI 413 GAATTC [GA]^AATT[CT]
446 GAATTC
Asp700I 442 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
873 GAAGTCTTTC
BanI 703 GGCGCC G^G[CT][GA]CC
810 GGTGCC
BseRI 187 GAGGAG GAGGAG(10/8)
469 GAGGAG
BsrI 738 ACTGG ACTGG(1/-1)
842 CCAGT
Cac8I 165 GCCCGC GC[ACGT]^[ACGT]GC
742 GCCGGC
EcoRI 413 GAATTC G^AATTC
446 GAATTC
HgaI 322 GACGC GACGC(5/10)
21 GCGTC
HinfI 34 GAGTC G^A[ACGT]TC
294 GATTC
SmlI 52 CTCAAG C^T[CT][GA]AG
466 CTCGAG
Tsp509I 414 AATT ^AATT
447 AATT
XmnI 442 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
873 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstXI CfoI Cfr13I DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI SacI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I Van91I