Restriction map for
pF1KB6484
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 1012 AAATTC [GA]^AATT[CT]
AfaI 120 GTAC GT^AC
AflIII 581 ACGTGT A^C[GA][CT]GT
AlwI 513 GATCC GGATC(4/5)
AlwNI 131 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1012 AAATTC [GA]^AATT[CT]
AvaII 124 GGACC G^G[AT]CC
BalI 1007 TGGCCA TGG^CCA
BbrPI 580 CACGTG CAC^GTG
BfaI 290 CTAG C^TAG
BlpI 430 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 430 GCTCAGC GC^T[ACGT]AGC
BsgI 88 GTGCAG GTGCAG(16/14)
BsmFI 39 GGGAC GGGAC(10/14)
BspMI 472 ACCTGC ACCTGC(4/8)
BsrDI 258 CATTGC GCAATG(2/0)
BsrI 630 ACTGG ACTGG(1/-1)
BssKI 84 CCTGG ^CC[ACGT]GG
BstAPI 435 GCATGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1044 GGATG GGATG(2/0)
BstNI 84 CCTGG CC^[AT]GG
BstXI 1010 CCAAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 430 GCTCAGC GC^T[ACGT]AGC
DraI 385 TTTAAA TTT^AAA
DraII 878 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 310 GACCGAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 452 CCATGG C^C[GA][CT]GG
EaeI 1007 TGGCCA [CT]^GGCC[GA]
EcoO109I 878 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 84 CCTGG ^CC[AT]GG
FokI 1044 GGATG GGATG(9/13)
HincII 409 GTTGAC GT[CT]^[GA]AC
HindII 409 GTTGAC GT[CT]^[GA]AC
HinfI 615 GAATC G^A[ACGT]TC
MaeI 290 CTAG C^TAG
MluNI 1007 TGGCCA TGG^CCA
MscI 1007 TGGCCA TGG^CCA
MslI 901 CATAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 84 CCTGG CC^[AT]GG
NcoI 452 CCATGG C^CATGG
NspI 435 GCATGC [GA]CATG^[CT]
PmaCI 580 CACGTG CAC^GTG
PmlI 580 CACGTG CAC^GTG
RsaI 120 GTAC GT^AC
ScrFI 84 CCTGG CC^[ACGT]GG
SexAI 83 ACCTGGT A^CC[AT]GGT
SphI 435 GCATGC GCATG^C
TfiI 615 GAATC G^A[AT]TC
XcmI 983 CCATTTATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 468 GTCTAC GT^[AC][GT]AC
962 GTCTAC
BsaAI 167 TACGTG [CT]AC^GT[GA]
580 CACGTG
BsaJI 35 CCTTGG C^C[ACGT][ACGT]GG
452 CCATGG
BsmAI 742 GAGAC GTCTC(1/5)
769 GAGAC
EcoT14I 35 CCTTGG C^C[AT][AT]GG
452 CCATGG
HphI 97 GGTGA GGTGA(8/7)
419 TCACC
MaeII 168 ACGT A^CGT
581 ACGT
MseI 284 TTAA T^TAA
386 TTAA
MspA1I 73 CAGCTG C[AC]G^C[GT]G
967 CAGCTG
NlaIV 80 GGAACC GG[ACGT]^[ACGT]CC
809 GGCTCC
PvuII 73 CAGCTG CAG^CTG
967 CAGCTG
StyI 35 CCTTGG C^C[AT][AT]GG
452 CCATGG
Tru9I 284 TTAA T^TAA
386 TTAA
Tsp45I 98 GTGAC ^GT[GC]AC
308 GTGAC
TspRI 91 CAGTG CA[GC]TG[ACGT][ACGT]^
341 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
Cac8I Cfr13I DdeI DpnII Fnu4HI
HaeIII ItaI MaeIII MboI MboII
MnlI MwoI NdeII NlaIII PstI
Sau3AI Sau96I SfaNI SfcI TseI
Tsp509I