Restriction map for
pF1KB6505
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI Hin1I HincII HindII
HindIII HpaI KasI MaeI MamI
MfeI MroI MseI MunI NarI
NciI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 385 GGTACC G^GTACC
AccI 279 GTATAC GT^[AC][GT]AC
AflIII 659 ACGCGT A^C[GA][CT]GT
AocI 469 CCTGAGG CC^T[ACGT]AGG
Asp700I 1057 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 385 GGTACC G^GTACC
BanI 385 GGTACC G^G[CT][GA]CC
BbsI 565 GTCTTC GAAGAC(2/6)
BciVI 458 GTATCC GTATCC(6/5)
BpuAI 565 GTCTTC GAAGAC(2/6)
BsaAI 673 TACGTG [CT]AC^GT[GA]
BsmAI 1049 GAGAC GTCTC(1/5)
BspMI 283 ACCTGC ACCTGC(4/8)
BsrFI 766 GCCGGC [GA]^CCGG[CT]
Bst1107I 279 GTATAC GTA^TAC
BstYI 815 AGATCC [GA]^GATC[CT]
BstZ17I 279 GTATAC GTA^TAC
Bsu36I 469 CCTGAGG CC^T[ACGT]AGG
Cfr10I 766 GCCGGC [GA]^CCGG[CT]
Eco81I 469 CCTGAGG CC^T[ACGT]AGG
EcoT14I 420 CCATGG C^C[AT][AT]GG
FseI 765 GGCCGGCC GGCCGG^CC
HaeII 747 GGCGCT [GA]GCGC^[CT]
HapII 767 CCGG C^CGG
HgaI 84 GCGTC GACGC(5/10)
HpaII 767 CCGG C^CGG
KpnI 385 GGTACC GGTAC^C
KspI 822 CCGCGG CCGC^GG
MaeII 674 ACGT A^CGT
MaeIII 518 GTCAC ^GT[ACGT]AC
MflI 815 AGATCC [GA]^GATC[CT]
MluI 659 ACGCGT A^CGCGT
MslI 349 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 767 CCGG C^CGG
NaeI 766 GCCGGC GCC^GGC
NcoI 420 CCATGG C^CATGG
NgoMIV 766 GCCGGC G^CCGGC
PstI 842 CTGCAG CTGCA^G
PvuII 899 CAGCTG CAG^CTG
SacI 101 GAGCTC GAGCT^C
SacII 822 CCGCGG CCGC^GG
SbfI 841 CCTGCAGG CCTGCA^GG
SfiI 243 GGCCTACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 547 CTCAAG C^T[CT][GA]AG
Sse8387I 841 CCTGCAGG CCTGCA^GG
StuI 846 AGGCCT AGG^CCT
StyI 420 CCATGG C^C[AT][AT]GG
TaqI 105 TCGA T^CGA
TfiI 640 GATTC G^A[AT]TC
Tsp45I 518 GTCAC ^GT[GC]AC
TthHB8I 105 TCGA T^CGA
XcmI 368 CCACTGAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 815 AGATCC [GA]^GATC[CT]
XmnI 1057 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 294 GTAC GT^AC
386 GTAC
AlwI 816 GATCC GGATC(4/5)
977 GATCC
AlwNI 250 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
269 CAGGCACTG
ApaI 112 GGGCCC GGGCC^C
205 GGGCCC
AspHI 101 GAGCTC G[AT]GC[AT]^C
481 GTGCTC
AvaI 226 CCCGAG C^[CT]CG[GA]G
711 CTCGGG
BalI 185 TGGCCA TGG^CCA
497 TGGCCA
BcgI 354 GCATGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
844 GCAGGCCTCTCG
BglI 244 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
263 GCCCTACAGGC
BsgI 256 CTGCAC GTGCAG(16/14)
931 CTGCAC
BsiHKAI 101 GAGCTC G[AT]GC[AT]^C
481 GTGCTC
BsoBI 226 CCCGAG C^[CT]CG[GA]G
711 CTCGGG
BsrI 628 ACTGG ACTGG(1/-1)
1017 ACTGG
BstF5I 924 CATCC GGATG(2/0)
63 GGATG
BstXI 8 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
500 CCACCGCCCTGG
DsaI 420 CCATGG C^C[GA][CT]GG
822 CCGCGG
FokI 924 CATCC GGATG(9/13)
63 GGATG
HphI 519 TCACC GGTGA(8/7)
755 TCACC
MluNI 185 TGGCCA TGG^CCA
497 TGGCCA
MscI 185 TGGCCA TGG^CCA
497 TGGCCA
NspI 354 GCATGT [GA]CATG^[CT]
428 ACATGC
PleI 613 GACTC GAGTC(4/5)
787 GACTC
RsaI 294 GTAC GT^AC
386 GTAC
SfaNI 957 GATGC GCATC(5/9)
923 GCATC
SfcI 266 CTACAG C^T[GA][CT]AG
842 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MboI MboII MnlI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
PpuMI SapI Sau3AI Sau96I ScrFI
TseI TspRI