Restriction map for
pF1KB6541
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MboII
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 389 GGTACC G^GTACC
AocI 557 CCTTAGG CC^T[ACGT]AGG
ApaI 858 GGGCCC GGGCC^C
Asp718I 389 GGTACC G^GTACC
AspHI 101 GAGCTC G[AT]GC[AT]^C
AvaI 53 CCCGGG C^[CT]CG[GA]G
BalI 661 TGGCCA TGG^CCA
BamHI 480 GGATCC G^GATCC
BbvCI 698 CCTCAGC CCTCAGC(-5/-2)
BciVI 419 GGATAC GTATCC(6/5)
BsaAI 73 TACGTA [CT]AC^GT[GA]
BsaI 588 GAGACC GGTCTC(1/5)
BsgI 218 GTGCAG GTGCAG(16/14)
BsiHKAI 101 GAGCTC G[AT]GC[AT]^C
BsmBI 231 GAGACG CGTCTC(1/5)
BsoBI 53 CCCGGG C^[CT]CG[GA]G
BsrDI 583 GCAATG GCAATG(2/0)
Bst1107I 804 GTATAC GTA^TAC
BstXI 470 CCAGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 804 GTATAC GTA^TAC
Bsu36I 557 CCTTAGG CC^T[ACGT]AGG
Cac8I 335 GCATGC GC[ACGT]^[ACGT]GC
CfoI 29 GCGC GCG^C
DsaI 878 CCATGG C^C[GA][CT]GG
EaeI 661 TGGCCA [CT]^GGCC[GA]
Eco81I 557 CCTTAGG CC^T[ACGT]AGG
EcoRI 957 GAATTC G^AATTC
HaeII 28 AGCGCC [GA]GCGC^[CT]
HhaI 29 GCGC GCG^C
HinP1I 29 GCGC G^CGC
KpnI 389 GGTACC GGTAC^C
MluNI 661 TGGCCA TGG^CCA
MscI 661 TGGCCA TGG^CCA
NcoI 878 CCATGG C^CATGG
PstI 287 CTGCAG CTGCA^G
SacI 101 GAGCTC GAGCT^C
SfaNI 703 GCATC GCATC(5/9)
SfcI 287 CTGCAG C^T[GA][CT]AG
SmaI 53 CCCGGG CCC^GGG
SmlI 252 CTCAAG C^T[CT][GA]AG
SnaBI 73 TACGTA TAC^GTA
SphI 335 GCATGC GCATG^C
TaqI 489 TCGA T^CGA
TthHB8I 489 TCGA T^CGA
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 653 GTAGAC GT^[AC][GT]AC
804 GTATAC
AcsI 957 GAATTC [GA]^AATT[CT]
1026 AAATTT
AflIII 241 ACATGT A^C[GA][CT]GT
800 ACATGT
ApoI 957 GAATTC [GA]^AATT[CT]
1026 AAATTT
BanI 389 GGTACC G^G[CT][GA]CC
552 GGTGCC
BanII 101 GAGCTC G[GA]GC[CT]^C
858 GGGCCC
BseRI 536 CTCCTC GAGGAG(10/8)
696 CTCCTC
BsmAI 231 GAGAC GTCTC(1/5)
588 GAGAC
BspLU11I 241 ACATGT A^CATGT
800 ACATGT
BstF5I 502 CATCC GGATG(2/0)
1005 GGATG
BstYI 207 GGATCT [GA]^GATC[CT]
480 GGATCC
DpnII 208 GATC ^GATC
481 GATC
EcoNI 538 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
727 CCTCCAGGAGG
FokI 502 CATCC GGATG(9/13)
1005 GGATG
HapII 54 CCGG C^CGG
991 CCGG
HpaII 54 CCGG C^CGG
991 CCGG
HphI 793 GGTGA GGTGA(8/7)
382 TCACC
MaeII 74 ACGT A^CGT
234 ACGT
MboI 208 GATC ^GATC
481 GATC
MflI 207 GGATCT [GA]^GATC[CT]
480 GGATCC
MseI 811 TTAA T^TAA
1033 TTAA
MspI 54 CCGG C^CGG
991 CCGG
MwoI 286 GCTGCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
920 GCTCTTTAGGC
NdeII 208 GATC ^GATC
481 GATC
PleI 534 GACTC GAGTC(4/5)
901 GAGTC
PpuMI 345 AGGTCCT [GA]G^G[AT]CC[CT]
723 AGGTCCT
Sau3AI 208 GATC ^GATC
481 GATC
TfiI 176 GATTC G^A[AT]TC
778 GATTC
Tru9I 811 TTAA T^TAA
1033 TTAA
Tsp45I 381 GTCAC ^GT[GC]AC
903 GTCAC
XhoII 207 GGATCT [GA]^GATC[CT]
480 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HinfI ItaI MaeI MaeIII
MnlI MslI MvaI NciI NlaIII
NlaIV NspI PflMI RsaI Sau96I
ScrFI StyI TseI Tsp509I TspRI
Van91I