Restriction map for
pF1KB6550
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspLU11I BspMI BsrFI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MroI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 372 ACGTGT A^C[GA][CT]GT
Asp700I 370 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1146 GAGCTC G[AT]GC[AT]^C
BalI 308 TGGCCA TGG^CCA
BanI 46 GGCACC G^G[CT][GA]CC
BanII 1146 GAGCTC G[GA]GC[CT]^C
BbrPI 854 CACGTG CAC^GTG
BbsI 1135 GAAGAC GAAGAC(2/6)
BbvCI 771 GCTGAGG CCTCAGC(-5/-2)
BcgI 552 GCAGAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 621 TGATCA T^GATCA
BpmI 918 CTCCAG CTGGAG(16/14)
BpuAI 1135 GAAGAC GAAGAC(2/6)
BsaAI 854 CACGTG [CT]AC^GT[GA]
BsaI 1022 GAGACC GGTCTC(1/5)
BsgI 640 CTGCAC GTGCAG(16/14)
BsiHKAI 1146 GAGCTC G[AT]GC[AT]^C
BspDI 836 ATCGAT AT^CGAT
BsrDI 279 CATTGC GCAATG(2/0)
BssHII 21 GCGCGC G^CGCGC
BssSI 1076 CTCGTG CACGAG(-5/-1)
ClaI 836 ATCGAT AT^CGAT
DraIII 1073 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 257 CCATGG C^C[GA][CT]GG
EaeI 308 TGGCCA [CT]^GGCC[GA]
EcoRI 522 GAATTC G^AATTC
FauI 914 CCCGC CCCGC(4/6)
FbaI 621 TGATCA T^GATCA
HapII 54 CCGG C^CGG
HincII 74 GTCAAC GT[CT]^[GA]AC
HindII 74 GTCAAC GT[CT]^[GA]AC
HindIII 670 AAGCTT A^AGCTT
HpaII 54 CCGG C^CGG
MluNI 308 TGGCCA TGG^CCA
MscI 308 TGGCCA TGG^CCA
MslI 598 CATGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 54 CCGG C^CGG
NcoI 257 CCATGG C^CATGG
PmaCI 854 CACGTG CAC^GTG
PmlI 854 CACGTG CAC^GTG
PstI 268 CTGCAG CTGCA^G
SacI 1146 GAGCTC GAGCT^C
SapI 1153 GAAGAGC GCTCTTC(1/4)
SfcI 268 CTGCAG C^T[GA][CT]AG
SmaI 53 CCCGGG CCC^GGG
SmlI 332 CTCAAG C^T[CT][GA]AG
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 967 TCTAGA T^CTAGA
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
XmnI 370 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 85 AAATTC [GA]^AATT[CT]
522 GAATTC
ApoI 85 AAATTC [GA]^AATT[CT]
522 GAATTC
AvaI 53 CCCGGG C^[CT]CG[GA]G
887 CTCGGG
BcnI 53 CCCGG CC^[GC]GG
54 CCGGG
BmyI 45 GGGCAC G[AGT]GC[ACT]^C
1146 GAGCTC
BsaBI 286 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
878 GATCCAAATC
BsoBI 53 CCCGGG C^[CT]CG[GA]G
887 CTCGGG
Bsp1286I 45 GGGCAC G[AGT]GC[ACT]^C
1146 GAGCTC
BsrBI 915 CCGCTC CCGCTC(-3/-3)
203 GAGCGG
BstF5I 221 CATCC GGATG(2/0)
468 GGATG
DraII 778 AGGTCCC [GA]G^G[ACGT]CC[CT]
910 AGGTCCC
EcoO109I 778 AGGTCCC [GA]G^G[ACGT]CC[CT]
910 AGGTCCC
EcoT14I 257 CCATGG C^C[AT][AT]GG
413 CCAAGG
FokI 221 CATCC GGATG(9/13)
468 GGATG
HaeIII 12 GGCC GG^CC
309 GGCC
HphI 114 TCACC GGTGA(8/7)
1072 TCACC
MaeII 372 ACGT A^CGT
855 ACGT
MamI 286 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
878 GATCCAAATC
NciI 53 CCCGG CC^[GC]GG
54 CCGGG
PflMI 36 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
186 CCATGACCTGG
PpuMI 778 AGGTCCC [GA]G^G[AT]CC[CT]
910 AGGTCCC
StyI 257 CCATGG C^C[AT][AT]GG
413 CCAAGG
Van91I 36 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
186 CCATGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BspHI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HhaI HinP1I HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RcaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I XhoII