Restriction map for
pF1KB5573
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AvrII BamHI
BbrPI BfrI BlnI BlpI Bpu1102I
BsaAI BsiEI BsiWI BsmBI BspDI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI HgaI HpaI
KpnI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 167 GTAGAC GT^[AC][GT]AC
AccIII 1904 TCCGGA T^CCGGA
AhdI 2498 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3082 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 130 ATTAAT AT^TAAT
AsnI 130 ATTAAT AT^TAAT
AspEI 2498 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2669 TGGCCA TGG^CCA
BbsI 1483 GTCTTC GAAGAC(2/6)
BbvCI 3069 CCTCAGC CCTCAGC(-5/-2)
BcgI 1871 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3296 GTATCC GTATCC(6/5)
BpuAI 1483 GTCTTC GAAGAC(2/6)
BsaBI 3024 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1360 GAGACC GGTCTC(1/5)
BseAI 1904 TCCGGA T^CCGGA
BspEI 1904 TCCGGA T^CCGGA
BspHI 3389 TCATGA T^CATGA
BsrDI 2115 CATTGC GCAATG(2/0)
DraIII 244 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1504 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2498 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2768 ATGCAT ATGCA^T
FauI 2809 GCGGG CCCGC(4/6)
MamI 3024 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2669 TGGCCA TGG^CCA
MroI 1904 TCCGGA T^CCGGA
MscI 2669 TGGCCA TGG^CCA
MspA1I 815 CAGCTG C[AC]G^C[GT]G
NdeI 3524 CATATG CA^TATG
NsiI 2768 ATGCAT ATGCA^T
PflMI 780 CCATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 777 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 130 ATTAAT AT^TAAT
PvuII 815 CAGCTG CAG^CTG
RcaI 3389 TCATGA T^CATGA
SacI 708 GAGCTC GAGCT^C
ScaI 1211 AGTACT AGT^ACT
SmaI 2108 CCCGGG CCC^GGG
StuI 1575 AGGCCT AGG^CCT
Van91I 780 CCATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 338 CCATCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2108 CCCGGG C^CCGGG
XmaI 2108 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1995 CGCG CG^CG
2261 CGCG
AcyI 1774 GGCGCC G[GA]^CG[CT]C
2705 GGCGCC
AspI 16 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCATGTC
AvaI 1672 CCCGAG C^[CT]CG[GA]G
2108 CCCGGG
AviII 1712 TGCGCA TGC^GCA
1793 TGCGCA
BbeI 1774 GGCGCC GGCGC^C
2705 GGCGCC
BclI 476 TGATCA T^GATCA
2639 TGATCA
BglI 1263 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2708 GCCCCTATGGC
BsaHI 1774 GGCGCC G[GA]^CG[CT]C
2705 GGCGCC
BsaWI 1817 ACCGGA [AT]^CCGG[AT]
1904 TCCGGA
BsoBI 1672 CCCGAG C^[CT]CG[GA]G
2108 CCCGGG
BstUI 1995 CGCG CG^CG
2261 CGCG
EcoRI 2176 GAATTC G^AATTC
3403 GAATTC
FbaI 476 TGATCA T^GATCA
2639 TGATCA
FspI 1712 TGCGCA TGC^GCA
1793 TGCGCA
HaeII 1774 GGCGCC [GA]GCGC^[CT]
2705 GGCGCC
Hin1I 1774 GGCGCC G[GA]^CG[CT]C
2705 GGCGCC
HindIII 1436 AAGCTT A^AGCTT
2245 AAGCTT
KasI 1774 GGCGCC G^GCGCC
2705 GGCGCC
MvnI 1995 CGCG CG^CG
2261 CGCG
NarI 1774 GGCGCC GG^CGCC
2705 GGCGCC
PflFI 16 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCATGTC
SapI 298 GCTCTTC GCTCTTC(1/4)
2913 GCTCTTC
SfoI 1774 GGCGCC GGC^GCC
2705 GGCGCC
Tth111I 16 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
730 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI