Restriction map for
pF1KB6567
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BmyI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CfoI Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MamI MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 289 ACGTGT A^C[GA][CT]GT
AlwI 460 GATCC GGATC(4/5)
BalI 116 TGGCCA TGG^CCA
BanI 1073 GGCACC G^G[CT][GA]CC
BcnI 1013 CCCGG CC^[GC]GG
BfaI 475 CTAG C^TAG
BlpI 672 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 672 GCTGAGC GC^T[ACGT]AGC
BspDI 865 ATCGAT AT^CGAT
BspMI 1106 GCAGGT ACCTGC(4/8)
BstAPI 988 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 848 TTCGAA TT^CGAA
BstXI 992 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 672 GCTGAGC GC^T[ACGT]AGC
ClaI 865 ATCGAT AT^CGAT
DraII 641 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 116 TGGCCA [CT]^GGCC[GA]
EcoO109I 641 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1008 GAATTC G^AATTC
HaeIII 117 GGCC GG^CC
HapII 1014 CCGG C^CGG
HpaI 229 GTTAAC GTT^AAC
HpaII 1014 CCGG C^CGG
HphI 198 TCACC GGTGA(8/7)
MaeI 475 CTAG C^TAG
MaeIII 213 GTCAC ^GT[ACGT]AC
MluNI 116 TGGCCA TGG^CCA
MscI 116 TGGCCA TGG^CCA
MspI 1014 CCGG C^CGG
NciI 1013 CCCGG CC^[GC]GG
NcoI 119 CCATGG C^CATGG
NdeI 737 CATATG CA^TATG
NspV 848 TTCGAA TT^CGAA
PflMI 639 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 272 GACTC GAGTC(4/5)
PpuMI 641 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 149 CTGCAG CTGCA^G
ScaI 248 AGTACT AGT^ACT
SfcI 149 CTGCAG C^T[GA][CT]AG
SfuI 848 TTCGAA TT^CGAA
SmlI 72 CTCAAG C^T[CT][GA]AG
SspI 51 AATATT AAT^ATT
TfiI 1037 GATTC G^A[AT]TC
Tsp45I 213 GTCAC ^GT[GC]AC
Van91I 639 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 986 CCGC CCGC(-3/-1)
622 GCGG
AvaII 642 GGTCC G^G[AT]CC
708 GGACC
BsmFI 451 GGGAC GGGAC(10/14)
162 GTCCC
BsmI 486 GAATGC GAATGC(1/-1)
545 GAATGC
Cfr13I 642 GGTCC G^G[ACGT]CC
708 GGACC
DpnII 460 GATC ^GATC
864 GATC
DsaI 119 CCATGG C^C[GA][CT]GG
716 CCGTGG
HinfI 272 GACTC G^A[ACGT]TC
1037 GATTC
MaeII 80 ACGT A^CGT
289 ACGT
MboI 460 GATC ^GATC
864 GATC
MslI 391 CATATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
993 CACTGCTGTG
MwoI 982 GCTGCCGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
988 GCAGCCACTGC
NdeII 460 GATC ^GATC
864 GATC
Sau3AI 460 GATC ^GATC
864 GATC
Sau96I 642 GGTCC G^G[ACGT]CC
708 GGACC
XcmI 119 CCATGGTCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
806 CCAGATTAAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
BbsI BbvI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsrI
BssKI BstF5I BstNI DdeI DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HincII HindII ItaI Ksp632I MboII
MnlI MseI MvaI NlaIII NlaIV
RsaI ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I