##################################
# NO CUT ENZYMES (  226 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AccI          280  GTCTAC               GT^[AC][GT]AC
AciI           67  GCGG                 CCGC(-3/-1)
AseI           52  ATTAAT               AT^TAAT
AsnI           52  ATTAAT               AT^TAAT
BsaJI          17  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI          16  CCCAAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           16  CCCAAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         216  ACCTGC               ACCTGC(4/8)
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoT14I        17  CCAAGG               C^C[AT][AT]GG
FauI           67  GCGGG                CCCGC(4/6)
HaeIII          3  GGCC                 GG^CC
MboII         241  GAAGA                GAAGA(8/7)
MfeI          248  CAATTG               C^AATTG
MunI          248  CAATTG               C^AATTG
PleI           26  GAGTC                GAGTC(4/5)
PshBI          52  ATTAAT               AT^TAAT
StyI           17  CCAAGG               C^C[AT][AT]GG
TfiI          176  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsgI          218  CTGCAC               GTGCAG(16/14)
               88  GTGCAG              
BstF5I         75  GGATG                GGATG(2/0)
              237  GGATG               
DdeI           35  CTGAG                C^T[ACGT]AG
              118  CTTAG               
DpnII          46  GATC                 ^GATC
              171  GATC                
FokI           75  GGATG                GGATG(9/13)
              237  GGATG               
HinfI          26  GAGTC                G^A[ACGT]TC
              176  GATTC               
MboI           46  GATC                 ^GATC
              171  GATC                
NdeII          46  GATC                 ^GATC
              171  GATC                
Sau3AI         46  GATC                 ^GATC
              171  GATC                
Tsp509I        55  AATT                 ^AATT
              249  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MnlI        MseI        Tru9I