Restriction map for
pF1KB6749
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstYI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MboII
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvaI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 137 GAATTT [GA]^AATT[CT]
AfaI 294 GTAC GT^AC
AlwI 198 GATCC GGATC(4/5)
ApoI 137 GAATTT [GA]^AATT[CT]
AspHI 223 GAGCAC G[AT]GC[AT]^C
BbeI 107 GGCGCC GGCGC^C
BbvI 66 GCTGC GCAGC(8/12)
BcnI 201 CCGGG CC^[GC]GG
BfaI 116 CTAG C^TAG
BmyI 223 GAGCAC G[AGT]GC[ACT]^C
BpmI 187 CTGGAG CTGGAG(16/14)
BsaWI 142 TCCGGT [AT]^CCGG[AT]
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
BsmAI 160 GAGAC GTCTC(1/5)
BsmFI 203 GGGAC GGGAC(10/14)
Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C
BsrFI 96 ACCGGC [GA]^CCGG[CT]
BssKI 201 CCGGG ^CC[ACGT]GG
BssSI 164 CTCGTG CACGAG(-5/-1)
BstXI 251 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 225 GCACGC GC[ACGT]^[ACGT]GC
Cfr10I 96 ACCGGC [GA]^CCGG[CT]
DpnII 198 GATC ^GATC
DsaI 251 CCATGG C^C[GA][CT]GG
EaeI 98 CGGCCA [CT]^GGCC[GA]
FauI 69 GCGGG CCCGC(4/6)
HaeII 107 GGCGCC [GA]GCGC^[CT]
HaeIII 99 GGCC GG^CC
HinfI 162 GACTC G^A[ACGT]TC
KasI 107 GGCGCC G^GCGCC
MaeI 116 CTAG C^TAG
MaeII 194 ACGT A^CGT
MboI 198 GATC ^GATC
MseI 131 TTAA T^TAA
NarI 107 GGCGCC GG^CGCC
NciI 201 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
NdeII 198 GATC ^GATC
NlaIII 252 CATG CATG^
PleI 162 GACTC GAGTC(4/5)
RsaI 294 GTAC GT^AC
Sau3AI 198 GATC ^GATC
ScrFI 201 CCGGG CC^[ACGT]GG
SfaNI 86 GCATC GCATC(5/9)
SfoI 107 GGCGCC GGC^GCC
Tru9I 131 TTAA T^TAA
TseI 66 GCTGC G^C[AT]GC
Tsp509I 138 AATT ^AATT
TspRI 246 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 10 CGCG CG^CG
267 CGCG
AcyI 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
BanI 43 GGTGCC G^G[CT][GA]CC
107 GGCGCC
BsaHI 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
BsaJI 81 CCAAGG C^C[ACGT][ACGT]GG
251 CCATGG
BstF5I 78 CATCC GGATG(2/0)
255 GGATG
BstUI 10 CGCG CG^CG
267 CGCG
EcoT14I 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
Fnu4HI 11 GCGGC GC^[ACGT]GC
66 GCTGC
FokI 78 CATCC GGATG(9/13)
255 GGATG
Hin1I 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
HphI 54 TCACC GGTGA(8/7)
89 TCACC
ItaI 11 GCGGC GC^[ACGT]GC
66 GCTGC
MnlI 41 GAGG CCTC(7/6)
157 GAGG
MslI 246 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATGGATGTG
MvnI 10 CGCG CG^CG
267 CGCG
MwoI 11 GCGGCAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
225 GCACGCCAAGC
NlaIV 43 GGTGCC GG[ACGT]^[ACGT]CC
107 GGCGCC
SmlI 175 CTCAAG C^T[CT][GA]AG
271 CTCAAG
StyI 81 CCAAGG C^C[AT][AT]GG
251 CCATGG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AciI CfoI HapII HgaI HhaI
HinP1I HpaII MaeIII MspI Tsp45I