Restriction map for
pF1KB6766
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HgaI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 386 GTAGAC GT^[AC][GT]AC
AcsI 246 GAATTT [GA]^AATT[CT]
AcyI 51 GGCGCC G[GA]^CG[CT]C
AlwNI 205 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 246 GAATTT [GA]^AATT[CT]
AvaI 56 CCCGGG C^[CT]CG[GA]G
BanI 51 GGCGCC G^G[CT][GA]CC
BanII 13 GAGCTC G[GA]GC[CT]^C
BbeI 51 GGCGCC GGCGC^C
BbsI 171 GTCTTC GAAGAC(2/6)
BglI 54 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 398 CTCCAG CTGGAG(16/14)
BpuAI 171 GTCTTC GAAGAC(2/6)
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsaI 256 GGTCTC GGTCTC(1/5)
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsmAI 257 GTCTC GTCTC(1/5)
BsoBI 56 CCCGGG C^[CT]CG[GA]G
BsrBI 65 GAGCGG CCGCTC(-3/-3)
BsrI 128 ACTGG ACTGG(1/-1)
BstXI 302 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 6 CGGCCG [CT]^GGCC[GA]
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
EcoO109I 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 302 CCAAGG C^C[AT][AT]GG
HapII 57 CCGG C^CGG
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HpaII 57 CCGG C^CGG
KasI 51 GGCGCC G^GCGCC
MaeII 411 ACGT A^CGT
MseI 145 TTAA T^TAA
MspI 57 CCGG C^CGG
NarI 51 GGCGCC GG^CGCC
SacI 13 GAGCTC GAGCT^C
SfoI 51 GGCGCC GGC^GCC
SmaI 56 CCCGGG CCC^GGG
StyI 302 CCAAGG C^C[AT][AT]GG
TfiI 334 GATTC G^A[AT]TC
Tru9I 145 TTAA T^TAA
Tsp509I 247 AATT ^AATT
TspRI 127 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 56 CCCGGG C^CCGGG
XmaI 56 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 111 GTAC GT^AC
219 GTAC
AspHI 13 GAGCTC G[AT]GC[AT]^C
159 GAGCAC
BcnI 56 CCCGG CC^[GC]GG
57 CCGGG
BsiHKAI 13 GAGCTC G[AT]GC[AT]^C
159 GAGCAC
BsmFI 91 GGGAC GGGAC(10/14)
70 GTCCC
DdeI 185 CTGAG C^T[ACGT]AG
211 CTGAG
HaeII 33 GGCGCT [GA]GCGC^[CT]
51 GGCGCC
HaeIII 7 GGCC GG^CC
306 GGCC
NciI 56 CCCGG CC^[GC]GG
57 CCGGG
PleI 389 GACTC GAGTC(4/5)
169 GAGTC
PvuII 208 CAGCTG CAG^CTG
430 CAGCTG
RsaI 111 GTAC GT^AC
219 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EcoRII FauI Fnu4HI HhaI
HinP1I HinfI ItaI MboII MnlI
MspA1I MvaI MvnI MwoI NlaIV
Sau96I ScrFI TseI