################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MslI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 400 CGCG CG^CG AccIII 123 TCCGGA T^CCGGA AocI 111 CCTGAGG CC^T[ACGT]AGG AvaII 403 GGTCC G^G[AT]CC BamHI 116 GGATCC G^GATCC BsaWI 123 TCCGGA [AT]^CCGG[AT] BseAI 123 TCCGGA T^CCGGA BsiYI 231 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 231 CCAGTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspEI 123 TCCGGA T^CCGGA BstUI 400 CGCG CG^CG BstYI 116 GGATCC [GA]^GATC[CT] Bsu36I 111 CCTGAGG CC^T[ACGT]AGG Cac8I 348 GCATGC GC[ACGT]^[ACGT]GC DpnII 117 GATC ^GATC DsaI 242 CCATGG C^C[GA][CT]GG EarI 456 CTCTTC CTCTTC(1/4) Eco81I 111 CCTGAGG CC^T[ACGT]AGG EcoT14I 242 CCATGG C^C[AT][AT]GG HapII 124 CCGG C^CGG HinfI 411 GACTC G^A[ACGT]TC HpaII 124 CCGG C^CGG HphI 17 TCACC GGTGA(8/7) Ksp632I 456 CTCTTC CTCTTC(1/4) MboI 117 GATC ^GATC MflI 116 GGATCC [GA]^GATC[CT] MroI 123 TCCGGA T^CCGGA MseI 47 TTAA T^TAA MspA1I 172 CAGCTG C[AC]G^C[GT]G MspI 124 CCGG C^CGG MvnI 400 CGCG CG^CG NcoI 242 CCATGG C^CATGG NdeII 117 GATC ^GATC NspI 348 GCATGC [GA]CATG^[CT] PflMI 231 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 411 GACTC GAGTC(4/5) PstI 175 CTGCAG CTGCA^G PvuII 172 CAGCTG CAG^CTG Sau3AI 117 GATC ^GATC ScaI 233 AGTACT AGT^ACT SmlI 376 CTCAAG C^T[CT][GA]AG SphI 348 GCATGC GCATG^C StyI 242 CCATGG C^C[AT][AT]GG Tru9I 47 TTAA T^TAA Van91I 231 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 116 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 187 GCGG CCGC(-3/-1) 401 GCGG AfaI 218 GTAC GT^AC 234 GTAC AlwI 117 GATCC GGATC(4/5) 116 GGATC BpmI 56 CTCCAG CTGGAG(16/14) 310 CTGGAG BseRI 82 CTCCTC GAGGAG(10/8) 313 GAGGAG BsmAI 223 GAGAC GTCTC(1/5) 454 GTCTC BsmFI 142 GGGAC GGGAC(10/14) 149 GGGAC BssKI 58 CCAGG ^CC[ACGT]GG 300 CCTGG BstF5I 126 GGATG GGATG(2/0) 138 GGATG BstNI 58 CCAGG CC^[AT]GG 300 CCTGG Cfr13I 102 GGGCC G^G[ACGT]CC 403 GGTCC DdeI 112 CTGAG C^T[ACGT]AG 181 CTCAG EcoRII 58 CCAGG ^CC[AT]GG 300 CCTGG FokI 126 GGATG GGATG(9/13) 138 GGATG MvaI 58 CCAGG CC^[AT]GG 300 CCTGG NlaIII 243 CATG CATG^ 349 CATG NlaIV 101 GGGGCC GG[ACGT]^[ACGT]CC 116 GGATCC RsaI 218 GTAC GT^AC 234 GTAC Sau96I 102 GGGCC G^G[ACGT]CC 403 GGTCC ScrFI 58 CCAGG CC^[ACGT]GG 300 CCTGG SfcI 90 CTGTAG C^T[GA][CT]AG 175 CTGCAG Tsp509I 64 AATT ^AATT 361 AATT TspRI 134 CAGTG CA[GC]TG[ACGT][ACGT]^ 183 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BalI BbvI BsaJI BsrI EaeI Fnu4HI HaeIII ItaI MboII MluNI MnlI MscI MwoI TseI